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6 Z [ [ R R R t t t (((222==<GGGRQQ[ZZdddmmmvv{~} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ac524820348a4469bb837020d19b9e0bSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-19_03-26_0744 3130Seq 12482 12482_Costion_K1322_matK_R jmadera 96-Well
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&KB.bcpKB_3730_POP7_BDTv3.mobtermAAAGGGTTTTAGGTGACCCGCCCCCCCACCTTAGGCCCTTTATAATAAAGAGAATTTCTCTGCTCCCAAGGCGTGCTTTAAAAATTTATGAGAGCCCCCCGGGCGTTTACCAATAGGGGGCCCCTCGTCCTAGTACTTTTTTTTTTCCAAGAAAGGGTTTTAGGTGACCCGCCCCCCCACCTTAGGCCCTTTATAATAAAGAGAATTTCTCTGCTCCCAAGGCGTGCTTTAAAAATTTATGAGAGCCCCCCGGGCGTTTACCAATAGGGGGCCCCTCGTCCTAGTACTTTTTTTTTTCCAAG # . ; I U e p &.=FOcmx &1>T^nw
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9. r vi 5 2 / H )i2 4 &' , T w I p {qxn k'' d )U< s pHp\pyw 4,<{] OvP 8 mT.[ ag . ^ ` M)mk4YHM Q d 2 ZAl[| u# ej2 B1 XC_wZ (*|x $?Q?ljP l&?+M j $8]_r1f=-k[Le(D@NCCGACTGGGGGATGAGTGGTAAAGTTGTTTAGTTCAGTCGGTATTGGTGAGATTCACTCTCATCGGAGTACGTACTCCATTTTGGACCGATAGTGGTTGGACCGCGCACAATCGGAAAACGGGTCACCGGGCAATAAAACAGCCAATGTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-19 04:57:07 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt < ASPt < B1Pt ; B1Pt ; BCTS f BufT # F 4 CTID CTNM CTOw $ CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA # Gr DATA
# Gr , DATA # Gr s DATA # Gr DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~
LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS u P1AM . P1RL . ` P1WD . P2AM . P2BA
F P2RL .
PBAS ? PBAS PCON PCON 2 PDMF PDMF PLOC . PLOC . PSZE ` PTYP , PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT 83 RUNT 0" RUNT 7
Rate RunN " " S/N% m SCAN 3 SMED
SMLt SMPL z SPAC M SPAC SPAC M SVER 4.0 SVER [ SVER , , v Scal @ Scan 3 TUBE D2 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt < ASPt < B1Pt ; B1Pt ; BCTS f BufT # F 4 CTID CTNM CTOw $ CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA # Gr DATA
# Gr , DATA # Gr s DATA # Gr DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~
LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS u P1AM . P1RL . ` P1WD . P2AM . P2BA
F P2RL .
PBAS ? PBAS PCON PCON 2 PDMF PDMF PLOC . PLOC . PSZE ` PTYP , PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT 83 RUNT 0" RUNT 7
Rate RunN " " S/N% m SCAN 3 SMED
SMLt SMPL z SPAC M SPAC SPAC M SVER 4.0 SVER [ SVER , , v Scal @ Scan 3 TUBE D2 Tmpr < User phAR phCH phDY big phQL c phTR phTR "3Z2VW)$>ZHm? V<(?:134]3[16Qu5r`xC>2aZ!ly^A9Jz?gMBI+\$jn18A7y!H#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;{v3Ah-8^#~C=O\rHU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nX>zt %v1?{6W8:7F}wxSB#_{=pQ$+Sn0&'*#))MR]N=]TAO$Cs-1G@cJ-omMOxq6agI)Y_bO?C!.tk,8#V>Ax7cXF$hHz))O1"K0n;DQZ`|rI35tF>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }OuRBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziPtJdQex-U[dPz;+hj3T'7_6t;:F8/FTO_s *i;}^;NF~7l"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"Bm`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQL AEPt 3 AEPt 3 APFN APXV 2 APrN _ APrV 1 APrX } } h ARTN ASPF ASPt < ASPt < AUDT d d + B1Pt ; B1Pt ; BCTS f BufT # F 4 CTID CTNM CTOw $ CTTL , CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA # F ^ DATA # F DATA # F DATA # F 0 DATA # Gr DATA
# Gr , DATA # Gr s DATA # Gr DCHT DSam DySN
Dye# DyeN DyeN DyeN " DyeN ' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~
LIMS ! ! 5 LNTD 2 LsrP a MCHN MODF V MODL 3730 NAVG NLNE ` NOIS u P1AM . P1RL . ` P1WD . P2AM . P2BA
F P2RL .
PBAS ? PBAS PCON PCON 2 PDMF PDMF PLOC . PLOC . PSZE ` PTYP , PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT 83 RUNT 0" RUNT 7
Rate RunN " " S/N% m SCAN 3 SMED
SMLt SMPL z SPAC M SPAC SPAC M SVER 4.0 SVER [ SVER , , v Scal @ Scan 3 TUBE D2 Tmpr < User phAR phCH phDY big phQL c phTR phTR