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6Z[[RRRttt(((222==<GGGRQQ[ZZdddmmmvv{~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6cedffe7d4de4e88b014612a14b8cf34Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_03-26_07443130Seq1248212482_Costion_K1322_matK_Rjmadera96-Well K1322_C3_RKB_3730_POP7_BDTv3.mobSeqPop7z #'+/1469<?ABDGKQX_eikmosz +AYp}unf\QC3! 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D>"FT 5/&J351?+MP/%IH\5/1533=c0/D3 X5S1-A321PDV1 543/.R6d3221%I3221!EL;_2Lew)_@_yYN^58s).xMe(MpbOC*(k9mlkn~y?o<0}Jo5:1v]kK3:?C~?Hl lT;iXCo 4y~$-L{wPjX <U.@8:VQ_|66%XSz[XADkrZ<{g+^f7_s W'ZuJAB  MTqdX ffx|U= M7xG'[NG  \"K,9d$3$JN$ CFY 5rb=WCV}:/T3o}CQ!p/(D +F&<r.~X3] p2BZ8B&r8pncf b1S v[i#d&jPz:r A2Zq=[q%R%t;6o;C~+f?gkN CSLNb@]5vDdBq1}bP%p @$QzgDR%.D.kEJI^,jGRzzPfzkrhyX[s-|N!w:d>z+n@| ;W3=\ymJKk?>UdwE@lyy{<WgHh Kl6  }fCXDW6(6B5DIC2:-78,3@=F+-2%+5#"!* 5                                          ,'"!#='$  &%!,+&%#!#!#!$--1(7220=007=;.70"@ED7@E<=+I:7<>S6K;=TDG96@FUYGD:GHAGNsdc]YDGQJVE>G5EdFMQ?SSESX;kkROKa^nRxyjgck^nd}orgXXXXltnuzQKo|I{ gype8QH2222222222 222L +ddd3d+++++d7Sddddddd1dd^ddddXXXTTTXXTBTNbXXh<KWbbTTTb4k! qL LLL LLLL/LLLLLLRR<$R>>iATDDRDDR>6jDsF &7 6ppR$pQ,Z%8/DN5,5pK d188JR,dbG&t'N+wI!p<F`K80pn,83Q#pMiB.AU+&,'ELppf"p8,,pRCa8ppCYpmppNMpppsdppppppppppppppYcpppje!Vpppppp a]\y4;vGe ju_{eM\zwr4#L8c]+1;:! YX[A#'fD0F:).7&4+J=-.1X>Y-0!1..C4 7YTE$O:#-!-*)FC *DE$'@!8%A6/! '!,2"0!(- */ !*&$"' 4"6(&- $1& # 3-+<&-&12$*(1 & 1>  (< (  $&@%5 0:) .-# ,D"-2 GqCb+- HZ5!8:&"C{3!K[&! U \}*Q 9L/$;-"E:6J. ER*=4(1:@AE\'J`LnD:]+B7wa~>F?&;VFRU%XS""u_;v*kQOn<by|fbVkZ* ynI+g}_dX23nemMWX he!|dT]m'A=)Nu61}Lg}Y/^7W~vzR\ xR# 'Ds1hR1 >-"_c pF' dA ;" R'r:Ja>)y/k 3$-1WV[ P-;ah+hFCx=v s  =['s p\DW)1y>To1'- Zhu6qz7!@[KrpG caT5\@1}@X4++ S4?&>+Q9 ATTAGTTTTACAGGGTGGAAGGGAGAATATACAATGTTATTACGAGTTATACCTCCACCTCTTCCAGCCTAGCGGACGTTTAGAATAGTATTCTATGTAGCCGGACAGAGCAGGATACTTAGAAGGACGGGCGGCGGTGCACCTTAGTGCTCCATCGACGGTGGGACTATTAGTGTTATTTCTAAGAAGGAAGTATCCTCCCTCCGAGGGGATGTATCTAATAGGGGAGGACTCTACCACAGAAGACTTCCCAGCGTCGATTTAGTGTGGACGCCTCCATGTATACCTTACCGTTTATGGGAAGTAGAGGGAGATTGGATGAATATAAATATTCCACACAGGTGCCAACCCACGAGTTTATTATACCTACAGTTAGAGGGGGTAATATACCTACACCTCCACATCAGGATAAGACTTTTCAGTGGAACGAGTAGCTCCCCTTCGTTATATCGCACATTACGCGAGGGAGTATCCGCACAGCAACAGAATGCTATGTGGGGAGAGACACCACACAACAATAGGGTGTGCTACACGTGGATATGCTATACCCCCTCATAGGTGTGTACTCTCACGAGGGATGCTACAGGATATAATTCTTATTAGAGGATAGTGAACAAGTATGCGCAAGTATGTTAACTTATACTCTTACATATGAGAGTATAGAAGCATGAGCGAGTCTCAATAAACTCAAGAGGGGCGTATTATATACCTCCTGAGTATGTGAGACACAATTCTAATAATATACGCGTGAGGAGTGCACATGTGTGTGGGGGAAGATCTCATCTTCCACAGTGAGACGACAACATACCTACATAGTATATATATGTGTAATAATATACTCTGACATATACATTTGTGTGATAGGTCTTCTCTCCGACAGGGGGTAGAGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 04:57:24 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTSBufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA ,YVDATA ,YV=DATA ,YVQDATA ,YVDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISJP1AM{mP1RL{fP1WD{sP2AM{P2BA{{P2RL{zPBAS{{MPBAS{{J8PCON{{bPCON{{_PDMFPDMFJPLOC{X$PLOC{Q.PSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%ISCAN3SMED SMLtSMPL zSPACAVySPACJSPACAVySVER4.0SVER SVER,,vScal@Scan3TUBEC3Tmpr<UserphARphCHJ3phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTSBufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA ,YVDATA ,YV=DATA ,YVQDATA ,YVDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab=FVoc,FWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISJP1AM{mP1RL{fP1WD{sP2AM{P2BA{{P2RL{zPBAS{{MPBAS{{J8PCON{{bPCON{{_PDMFPDMFJPLOC{X$PLOC{Q.PSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%ISCAN3SMED SMLtSMPL zSPACAVySPACJSPACAVySVER4.0SVER SVER,,vScal@Scan3TUBEC3Tmpr<UserphARphCHJ3phDYbigphQLcphTRphTRqk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|T4}@^B@68 toH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{Z(cOH?3+]g:+PZ+h$d/k>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtAUDTddPB1PtB1PtBCTSBufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA ,YVDATA ,YV=DATA ,YVQDATA ,YVDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab=FVoc,FWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISJP1AM{mP1RL{fP1WD{sP2AM{P2BA{{P2RL{zPBAS{{MPBAS{{J8PCON{{bPCON{{_PDMFPDMFJPLOC{X$PLOC{Q.PSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%ISCAN3SMED SMLtSMPL zSPACAVySPACJSPACAVySVER4.0SVER SVER,,vScal@Scan3TUBEC3Tmpr<UserphARphCHJ3phDYbigphQLcphTRphTR