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  9[[RRRttt'((222==<GGGQQQ[ZZdddmmmvuv~}}<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 16b26268ead446d8b5ee8979098cebccSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_01-55_07433130Seq1248112481_Costion_K1322_matK_Fjmadera96-WellK1322_E2KB_3730_POP7_BDTv3.mobSeqPop7zt    Z*Z^oHFf&@*} r  j)\mir[s7(Gf* ` { l"&*#-_0x369j;=g>6>7=,EOG/;QS>vVPm/v|n%%%%lJ1Q401I7"Vyh<f}~lL) r8[uYh9|wQay6~wq !JP1\.Vl [:[\4vu+&z%>VRJ '+!,I`gY<6gX2qz"9Yg^A,*[+t[?5JQG0HL=m w5Z=AbrQ!\ H3=-;l<1q  [y  o6 -Pj{ 3[k*/G< {PYn gi fPD{qD<JtaU  1[|zV-+3)ER +_~|@G4 >UXBc:?`g 6e8gs{?r@,-CE 8gY*2VljS2 $(% 6OYN/ 7a31TU'`xBCdvzuqt{ziSGPq;sGpU6 2df7   +V]6  V 58_{d?  &MvT# F:m_=) g%4wrXQ]w?|iaw3 ,PhncM6" 5h=]OwB{#em'*9|`$FHx*ZRc1)=RR86At6=-mG'&C[Z4=iZE+2! 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2NbkeUB1&+Lem`E% HylPC@B>2 ,:>5#$*$'?ON>%   ,53'  '-*" 5WqwgG" $%$&2Id{pV;%  &?``2  'FihJ4)).20'  /@HE<2/7EQTL?68BLJ7&ff,&Mt}R#=cwrV2  3K^jpt{iG%   9LUPA-/Nt}J'=F@.+@QWTKBBOd{gA $;FB0 1AIG=*)eX60>QXN4 'E\d\I2Hx`. &' =eiG/*:VoynR/ "-1+ $E`lhXG==BC9# Bh{sQ$ ;mk9D{Z'5O]\M3+WwL"    ,VvoQ;@bQRw<0Oiwt_@-E\oyvgM-JF +-&  #>RVF'+eWAh{sS((X~~]:%';R_YD*&7CC6" AiZ. 6OZUA& */()Re% 9ms@?{_* 8]x|jJ'  '25- %HftkN*$35(#0@F>, .<DC;/$   $8FMPSZdkiZ@"  '//'  1I[aZH1 ,?JH;)    +ANJ7 7@b/@\)A sABq AKB.bcpKB_3730_POP7_BDTv3.mobtermGACATTACCCCCAAGTAACTAACGTAATTACTCTAGATCGGAAAAATCGTATACTACCTCCTGCTCTGGGGTAAAAAGGGGGCTGTTCTGCGGGGGAAAGATACCCTCGTGCGGCATTAGATGGTTTTACATGATGATGAAAAGAAGACATTACCCCCAAGTAACTAACGTAATTACTCTAGATCGGAAAAATCGTATACTACCTCCTGCTCTGGGGTAAAAAGGGGGCTGTTCTGCGGGGGAAAGATACCCTCGTGCGGCATTAGATGGTTTTACATGATGATGAAAAGAA)0>HQ]gp,;DNXhv*3<JWbp|.>ITgs|"2>EP]iu!1COeq|!.9I]iy$0>FR`o)0>HQ]gp,;DNXhv*3<JWbp|.>ITgs|"2>EP]iu!1COeq|!.9I]iy$0>FR`o                                                              -X,. b%#<c0E"4_<@7MI .0<)5:UGN1=:;T]I>=/3ZIWTY UT  R+ 2* C;NYOaW=5Ob8b^<?(6 *5"8*#'LH,?'H0N'X^JC]3XMDE(6=S L )u  T A@ p  o PNBpz:p|b0?"F2#QxG.;=ro"t %U4L';:{!/UDGtJSjUy.E 9,_>J>|4T AQua$&0 *Wk:>40@ $$B ,h)Z a"NM1+k#=O3Za kd0M-uS5WsM\dJk_"vEA'+iYTjU1 %/FWC(?)+w .N2G']R+v7QHveykB's/y s2eT 9?LoKuFqo@!'WG>95=Nbz:H"=CGATAATTTAAGGTTATGAGGGACGCTACTAATAGATGGTTGGTGTAATACCCGCCTACTACCACACTTACCCTTTTATTTAGCTCCGATTTTTTAGGGAGAGATTCTTGCGTTGGAACTGCTTCCACCACGCGGTTGTGGGGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 03:26:25 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS BufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #RFDATA #RF+yDATA #RFrDATA #RFDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ vLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISmP1AM$TP1RL$0P1WD$xP2AM$P2BA P2RL$PBAS2PBASPCONPCON PDMFPDMFPLOC$PLOC$PSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%eSCAN3SMED SMLtSMPL SPACMSPAC}SPACMSVER4.0SVER  SVER,,Scal@Scan3TUBEE2Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS BufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #RFDATA #RF+yDATA #RFrDATA #RFDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabYFVocHFWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ vLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISmP1AM$TP1RL$0P1WD$xP2AM$P2BA P2RL$PBAS2PBASPCONPCON PDMFPDMFPLOC$PLOC$PSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%eSCAN3SMED SMLtSMPL SPACMSPAC}SPACMSVER4.0SVER  SVER,,Scal@Scan3TUBEE2Tmpr<UserphARphCHphDYbigphQLcphTRphTR&BQ{6Q2\gDT]U'Q>lW+UfyKnY18ds]`?Ne4sX$9WK]}DV&[>:)II;?1EFiRCwh1nk-~4Kb}wLD7X{u./uX8?;pPJwiO*$]_`#?QI}yn%/k%S>p=6"4#OvR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;8)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lsXW}0{vMa`HUX;FEX[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9uoW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'t*?_:xslBEBuECcO58QiyZ$wpor9Sqwg5j*"%c:22QX43:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NudrVLgJRv)1mfc4hApvaUG