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f x O H ^ M = @ < L @ B 4 E C F B C E D O B G C B i l [ i V Y P ` V R A C L W C M H W h w t n N V T M ; = ( 3 + 7 4 0 @ % 9 2 ; 2 = 0 , / , 2 1 * . # 4 - " + % 5 5 $ # . 6 2 2 > L H ? C M : : 8 G B O J V K ; ? < F > F ? ; G Q V { r i X P D C 9 P J H N G / : ; G J J ; 3 4 C ) 2 5 7 4 , - 5 ) < 5 : 7 # 5 2 D F P R j m o u q V J K D R I J F H B ^ j h e s l p e _ _ D _ C N > J 3 1 6 = % 6 9 7 7 C 9 2 . 8 # - - - ( 0 # @ 2 ) = ' / 1 * < > E . G J 9 7 < 5 . ) ( 3 E D 7 K X ` g q c P F E G < : , 9 D 3 6 0 D M @ 7 I 4 C 2 - 4 - 9 ? . - 3 + 4 ! ) ( / - ' & * 5 @ . @ > 2 B > 2 Q K > 7 L [ l h U B H I > - 6 8 ' : 1 6 ! / ' , ( + . , # # 2 = - ' # + $ 4 - $ # + # # $ + + ) & % + * # * + 0 - % ' ( & # 3 ! $ $ ) - = 6 @ 7 / , 2 3 % ( / ' ) & " - $ " ' 0 ! + & ) # % % % 5 ( $ ) 0 - ; 7 . ' % 3 7 ; ) - - $ - 7 ' ' ) ) * ' " * ' - % 2 : = Q 8 0 5 / F C 6 / : 8 & ) = ? = H J x g [ U p k I Q 3 D 9 C C F : ; 2 L 0 8 % / 2 > B & 1 . ; 2 F 7 . . 8 ) % 6 9 , 2 " - ( ) ( - , ( , - - + ( 1 A 6 9 5 6 H 8 7 > . 5 9 A 8 3 7 4 G J B 5 D A @ M 4 7 B E - ' , 2 1 % ; 4 , 1 ) ( + 6 + < 2 / , / . & * " / ( ( % ( & . - - . * 3 ' 1 ! ! ) 9 , / 4 8 ( $ - * ' > > 5 K Y _ l s P X F R A 3 3 , 9 ? , : : * $ ( / ) * / 9 . # , $ F 5 6 + ) * , + $ . 7 / 1 1 D ) * . . # + 6 - * # " 7 * 8 . = , : . , 0 6 ) = 8 < 1 0 * " 0 ( , 6 3 ( $ 0 3 B 7 F 5 B T W b x v d ; G 1 L 2 " - ; % + * ' 4 * $ . 0 * ' 0 , + $ ! - % 1 2 ' / + ' % % * ( ! . ' , 2 + ! 9 " - & 6 * ? 8 < < 5 6 : > A 6 " ' 2 7 + A 6 5 8 2 ) 3 A B Q a Z y z s { n k b S @ U < : , ! 2 , 5 ) % ) ' " 0 7 5 5 & ( ) / 9 + ' : / 0 4 & - ! + 5 ! ( * + 6 6 + > 4 ' ) > F 0 1 8 & 1 & 0 9 6 ? 3 V B O G F J > C V S M i n | t s p p e P F > = O ? : G 9 E 7 % ' 4 9 A 9 , 0 # C ; ) 4 & $ - ) 5 $ ) # C ( 5 % - 4 3 + % 9 $ 1 ) 6 * % 1 ) / " 7 ) + 6 ( 4 4 - 4 - D ( * 3 : - = 9 7 > 1 K D : T ; K I < C < = K H = = ; ? B @ / 7 0 5 . * & & , + ) # 4 ) % < 5 C 5 : - = + ? + I 6 5 ( ( - * , 1 2 8 5 E A h Y p } v Y O E [ 3 E 2 ? ( E # 3 5 + 2 > ( 0 $ & : 2 4 5 8 . D , 2 & 1 % + * ( 3 6 / $ " 0 . 5 4 ( 0 1 # 9 F ; * E 3 9 < L A J g t y p m [ [ T J M V = J ^ S K M M ^ e j S l V Q : < K F H B 3 = ' = 9 1 " & 4 % 0 ) % - 3 # C # 0 9 + / 4 - 1 8 8 - 1 -
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 970c649db19447deabdbb8bc26ccd8c3Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-19_01-55_0743 3130Seq 12481 12481_Costion_K1322_matK_F jmadera 96-Well K1322_E12KB_3730_POP7_BDTv3.mobSeqPop7z L L K M R \ k } &(%&3Ov
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CTTTTTTCTTATTTAGGGACGAGCGTACCTTATGTTAATGACAGTAGAACATAGGTACTCACAGTAGGGTACCTTCCTCTACCCATAAAAGCTTTTATTGAACAGTAGAAGCTGGGAGATTCTTCCTTGGTATACATGGCGAGAGTCTCTCTCCTAGAATAGCAGGAGTGGCATACTCTCAACACCACGGGGCAGTATCCATTTCCCACCTTCTGAGAGTAACGGACAAGAATCGACCTGCTAAATGACTATTGAGGGGGAGGACAATGAGTCCTCCTTGAAAATATGCGTCCGCCATTCAATTAAAGAATAAGGTTCTCCGGTTCAAGACCTGGAGCACCCAGGCGCGTCTAATCGGGTAGATTACATAAAGTTATAAATCGAAGTTAAGGACCTTAGGGCTTTCGGTTCCTCCTTGGACGTTCCAAGAATTACCATCAGTTATATTTTATGGCTAGTTACTATTTCCACGAAACTCTCTTCTACTCCTATGGAGGGAAAGGTCAAACATGGAATCTACGGGTATTTGACTTCCCGTTACTGGTTCTAACCTTCAGGGTCCATACATATGGAACTAAGATATCAACATAGCCCCTCCACGGTAAGGGTCGCCAACGAGCTCCGGGACACTCACATGCGTCGAATACACGAGTGCGATAGATTAAGCTAACTATAATCTACAGCTACGTCTGTTCTAACGGACAGCTGACGGTGACTCAATTCCTCCCACTCAGCTGTGTAACAGGATATCGAAATATATTGTATTACAGAATAGCGGTATACAATGAGCCATATCTTGTCTGAGAAGTGAGGGTTGGAGACCTACGAGTATCTTACGAATGTAGTGTAAATTGTCAGTTCTTGCCCTTCGTCACATGTAGGATCACGTGAGGTCCATCGAGGCTCTTCAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-19 03:27:45 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS }2 BufT ( P \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA *] T DATA
*] T 9 DATA *] T J DATA *] T DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE X LAST ~ ~ { LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS 7 P1AM [ P1RL Ty P1WD b P2AM p P2BA x P2RL i PBAS ; PBAS 7 PCON P PCON MY PDMF PDMF 7 PLOC F9 PLOC ? PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 70 RUNT
RUNT 5 RUNT Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL
SPAC A_E SPAC 7 SPAC A_E SVER 4.0 SVER }' SVER , , Scal @ Scan 3 TUBE E12 Tmpr < User phAR phCH 7 phDY big phQL c phTR phTR 6^ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS }2 BufT ( P \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA *] T DATA
*] T 9 DATA *] T J DATA *] T DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc | FWO_ GATC Feat / / M GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE X LAST ~ ~ { LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS 7 P1AM [ P1RL Ty P1WD b P2AM p P2BA x P2RL i PBAS ; PBAS 7 PCON P PCON MY PDMF PDMF 7 PLOC F9 PLOC ? PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 70 RUNT
RUNT 5 RUNT Rate RunN " " S/N% 7 SCAN 3 SMED
SMLt SMPL
SPAC A_E SPAC 7 SPAC A_E SVER 4.0 SVER }' SVER , , Scal @ Scan 3 TUBE E12 Tmpr < User phAR phCH 7 phDY big phQL c phTR phTR TFo@L15FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(<.1A!h$jU4MOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhHO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX>V