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y h j l q v e r w e ` X V X L > > C B 5 3 7 T E ] ` ] v h \ ] O V M J ^ 9 9 7 5 , . $ & 9 3 8 0 - ; 4 9 " . % $ - 0 1 6 1 6 % 3 % 1 1 5 0 2 & J 5 B 4 6 = + ; + 0 : 8 % ; B = C L G A @ B I G D V A ? ? G G ^ W @ R K _ ] R V S f ` j W q u r ~ f t { u n Y U X I M F 7 3 7 2 9 7 D F ; M N P 6 I 5 3 ; C : L 4 > 1 = 9 L R : U Q E F M Q J e f [ p i h ` T ^ T P O D Q Y \ [ ^ N g Z { -9B?) } %9/ /. y z h ` U M K H O E @ S G B H F 3 5 9 B @ H 0 5 A D F D J J R S V F Q U U K C * 9 > ? 4 5 , 8 D B ; B ) > C C R ] d w _ [ R Q G K = 3 ( 4 + ( 5 3 + B 8 9 + 1 3 7 4 + * 0 2 5 / - 5 B ( 3 ( 9 + A > A . B B E D 1 / / $ . : ) 8 + < 9 / 4 : ' 0 @ 1 3 1 F 4 5 / 6 / % ( - & ' + ( . + + , ) B 7 > / 3 < . ) # 5 & 9 O I Q O I < G H E < C < A G < D = O V ] i k z o O O P P Q E S / ) @ 3 7 $ + 2 2 * & - " 2 ! 8 1 6 . . / ' # 1 < % . $ B G > 8 = 7 8 7 / 8 < F D ( ? 4 C B I @ 9 ? ? 7 T O R ] s m ` n e g ] Q R S L d T W Z [ ] F ^ = L S T u h j ~ { u u v s d g N N I : 7 O N H E J ; B K B S : W ` r & x h | ` W ? D U ; B ; 1 > < 2 ? / ` H R S [ T g t z s j j h ^ P G B J ; @ G 0 = C B 9 A 8 : @ B C D < I F b > < ? Q D C J M O B : U K : @ N G F ? ; 9 3 / 4 0 ? B P 9 7 # D = 5 3 8 ) 0 2 1 % 5 , 0 - 3 ' 0 ? > A C E C 6 4 < 4 ; = 7 G 4 4 ; ; A ? < 6 + 6 0 = > D 3 @ 2 @ A / 6 5 ) 7 . = 6 E ; 5 K N 3 8 F ? G < M J U < E R H I H K F @ E D B O U Q J F R L O H H @ 5 2 K 4 3 ( : > D 5 6 R E @ W d m u v w a e C G C B ; 2 1 , 7 . , . + + 7 < 1 D ; * , ) C ( ; . . : 0 1 4 2 ? 3 : > 4 1 > ; ? A ? 2 F 6 8 < ) , ; 6 < - 4 6 F , 9 5 , 0 3 6 9 < . : 1 - ( 9 " . < " 0 3 - . + 3 4 ' / ' . 1 5 . 5 / . % > ' 8 0 * ( 1 4 0 8 1 0 0 ' . + , ( # , 9 0 & * % 6 ; , + , , % % 3 / ) 5 3 * - : 2 / < * 8 ; # ) * 2 ! ) + 0 = / ' # , 0 9 6 6 C < = @ ? F P G 1 4 1 < 5 > 6 3 / + 6 1 5 = / 9 . 1 8 W P ` l n x { e X U F S C C E 4 : > / : 2 B 9 ; ' ) A ; . A 9 2 C A 5 @ B : = J 6 ; > 8 > @ D J [ W M Z g c S d V b X N N n a v ~ y o h m U a x w ~ s v _ i q i n S T M ^ W < I O W e h d b c Z [ H W = D U = L M G ? 2 : ; 6 A C _ W ] f x r } h c ` Z R N E ? G : < 1 P 1 4 ? ? ? < L W K i ~ | { g j Y T e c ^ L b T Z [ S W ] S B N P I Y O V T O E L H I ` Q J C F X O V U m w y } g l P f U G L P B C = : O K > C 8 O @ N q W n ~ w t V \ U S Y R ? J A O J I 0 C P Q O L A J R L a ` B O G N K B H S U K d U T Y _ V K V R R Z P a _ d | t \ ` j D ` V Q V f p T R I a K \ T ? : L N K ] ^ o s \ k ` W J Z ] F Y T a t ~ { m m P T \ P I : : E 7 D C F O I D B I E H A D J ] T Z C C ? I D M H I K V S X F X P ] O W R > = M I Q O ^ N ^ v z x { W _ N V A I B A Q < E D 9 E H G H O 1 8 ; N A < D 6 @ W W ; = Q J D N [ k S M N V G = J H K 2 ? : < > S ? M > > F B U K E Y S l e z v w m e j R X R Q D ; H 5 > U F @ < > : 9 A J . 7 1 / @ 4 < > 8 : ; 9 . E 1 . A < ^ _ _ y o k _ \ _ S C 9 C ? E R E B ; / H E * J 4 = G J I O ^ [ l u ~ { b Z [ M b Z _ K I C H C B G C < Q 9 C L 9 F S Q < B T g m p ] W X k Z ] F D M A ? D G E M = ? W F M Q R E = - . 2 ? 9 7 3 5 ! 4 - / 8 6 & H ; < = 8 9 6 5 ? 9 : 5 3 1 . = 7 : 2 3 = J H < - < A 5 ? @ , > 9 ' B ; 1 2 ; 6 H C E B N N N F J E I N B H : H L B V S \ G P R V T _ R ^ [ a i d e ~ v y v d Q F I O 0 H I = 1 1 4 E = 7 * < 7 G E N N _ k s v y r h I K L 5 > 8 3 5 . ? 8 6 8 1 4 7 3 # 2 - , 1 5 - & 3 6 : D > @ ? K F P ; . . ? 6 1 . 2 8 > 6 0 A 1 : O @ 8 b N E J G 9 = 7 @ c \ I Q P k a } { x t j Z @ M D K 7 4 2 1 @ ' ' : 4 0 0 . , ) < : # 2 ( > 4 $ 9 - ( 5 ) ! 0 = * 5 % 2 . 7 # 0 1 ) / 3 / 7 ; ? / , / 7 0 7 8 = 7 " ( # . - + < * - 4 6 ) 2 ' * 0 ) & ; 6 - . @ C 2 * B 8 1 ? 1 ? 5 7 2 1 < 3 * ( , , 0 6 - 3 * / 3 5 2 - 2 3 3 5 % + 7 > : 9 @ P G j t } { h ] [ Q \ = ? 5 ) 1 ) . 2 $
& 6 1 & ' 3 + : ( 6 " ! 4 4 . - & ) * " + % 0 ! ) , 5 ! * % " * % & * 8 * " ) # - 2 ) . ' % $ $ : : 0 - 7 & + " @ 6 3 - ! " " - $ ' # ) 8 * & % ( & , ! - ' & ( 5 ' , + , ( & ) . + ? ) ) + 2 * - 6 ) - ' ' $ 4 / & + % % . $ 1 % ' % 5 $ 0 # * 3 * * * 2 1 ? * # , % 1 3 5 ; . 6 ( 8 5 , > D : @ P S c v t i Q W I ? ? * ; 6 1 , = 6 . E . > $ 8 - B 0 : F 7 J D G L b n s b R Q F 7 7 B 3 ) & ( G @ 4 2 . C 5 / 0 / & 7 6 9 C 0 I < ) ? M F * 2 1 6 < - # < 9 ( 9 + / ' 1 ! " $ * ' 0 ! ) ( " & G , . ' 1 & ) , 0 . ' % +
2 ( 1 $ . & 4 * ! 3 + < 2 7 A : 0 ( 5 @ % ( % ; 5 , & # . ( : 0 # 3 0 * 6 0 * * 1 3 / . 4 A 8 A O 2 H A Q Z a y s w t j o T P Z 1 2 6 0 / 1 6 2 3 / / 4 C ) 9 9 5 J > C , ! ? @ 1 K ( * 0 ) / + , ) 8 6 0 7 = A I > ; / : C 0 7 > B A J C O ` l r y | n v ] ] ] N > 9 ? ? 4 , ) * 9 6 & # + ) & " + / 3 ' 1 # / , 4 4 & 1 # 5 ( # & " ' # ' " 1 3 1 = 7 + % 0 C $ 4 0 C + 3 6 @ 9 6 ) A 5 4 7 : B 2 5 9 % 4 8 = 2 B ( ; > F A H B - = ) B # - 7 ' 7 - ) " F ' # 7 0 0 3 . 8 ! ; 3 2 5 6 9 . @ = G 1 L j k v | x n i a V O K I 1 I / ' ? 2 * * * + # : $ % 1 & 0 : = , = + ( = 0 0 0 J C L C 4 M H 4 K K U D H ; C K I < ? 0 4 7 O = @ G J I F D E E 7 H D ; F = X D A D > T G T Y f t t ~ b k T E H @ 3 * + " # # & ' - " % # " 3 ! " ) $ 0 ! 9 2 6 2 ) , 4 - + , , " * 0 + % ) , ! $ ) 0 ( & ( $ 5 ' 0 9 8 H = 2 @ : . A , C - 9 6 < 1 1 A 1 7 , ? A , 1 9 D 6 = C 6 G < 1 + / 1 ) 8 , ! ) " " 1 % # 1 ! ' & , ) . ? . C ; 6 D F G K G k ` g y v v y r q \ ] S F A 9 G 1 3 ' * H " 0 % 2 ' % * * ; * 1 1 , 6 * * 8 3 : 3 D * . K . H J > : 1 0 8 D 3 6 ; 8 ; ? & 4 . % / G @ Q 9 * 5 0 1 D F G 7 > J 6 N F G : P ] [ G r w r | ~ x | | z } t V n ] J V = H N B / 1 A 7 R B S = B 4 ; 2 ; I : 8 6 E G ; K Z a c w w u g h ^ M F D > E > = = 1 @ 9 + & + # + K 6 ' 7 % 9 ; ? * 9 - C = 1 0 7 D 4 2 2 K K U ` b o k m i o l m N F O , ; - * / & 5 * 8 + + ! ) / & / & & # * ( ! 6 7 2 @ / = = ? 6 , $ , ( ! $ ' # 2 " % & 0 $
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fc8f8cafa7b64be4b997e3c582c004f7Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-19_01-55_0743 3130Seq 12481 12481_Costion_K1322_matK_F jmadera 96-Well K1322_B8KB_3730_POP7_BDTv3.mobSeqPop7z s q o o q x y s o p w
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Lg> NNFw1b^I G5 ? A 5n r aNU `++ O8 o )cOX S`\3ACAAAATCTACTTATTAGGGAGCAAAGAATCACACGCAATTTCATTGAATAAGTTATACTCTTACATTCCAAAGTCCACCTGAACCCCTAGAAGCACCCTCTCCGTTGGGGATAATGGGAGACTTCTCCTAAAGTTAAATACGAATTCTCCCCCAACAATTATGGAATTGGGAGTGTCGTAATACTTCAACGGACTCTTTTTACCTCTTAACAACGTCTCTTTCAGGGTTCCTCCTCCTCACAATATATTCGAAAGAAATAGAAGGAAAATTATTTTCCGTTATCCATATTCACCATCCCTAAAATTACACAATAACTATATCGGAAAGAGTTCCTCACGCATGTATATAAAAATAAAGTTTTATAGCTTGGGATGTACCTCTCCCCAAGGATTACAGGGACGCCTTTATGATGCTAAACGCATATTTCCTTATACACTGTGAGGTGTCTTGAAGGTTAACCACTCCCTCCACCCAACAGACCCCTCAGGATAATTGGGAAAGCAAAATTTCGAGTTACAGGTACCTATGTTAAACGGCGCTGCGCCCAACACAAGTAAGATACATATCAATAATATACCTTCCCATGCGAGTGGTATTCTCTCTAGAACCGTGCGGTCTGTATTACTCTGATCACAGGTCCTTTGCACTTACTTCCTATATCATTACGCTAACCGGTGTGGACATCCTTCTATCTTAATCGATAGAGTACTATCTCTGGATTCCAGTGTAGTTATAAGATTTACGGAATCTCCGGTTGAGAGGATTCGTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-19 03:27:20 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS : BufT ( P \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA #] F DATA
#] F + DATA #] F rI DATA #] F DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ 91 LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD $ P2AM 0( P2BA 6. P2RL *" PBAS PBAS PCON
PCON
PDMF PDMF PLOC PLOC PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 70 RUNT
RUNT 5 RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER : SVER , , Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH phDY big phQL c phTR phTR 2 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS : BufT ( P \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA #] F DATA
#] F + DATA #] F rI DATA #] F DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab I FVoc I FWO_ GATC Feat / / H GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ 91 LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD $ P2AM 0( P2BA 6. P2RL *" PBAS PBAS PCON
PCON
PDMF PDMF PLOC PLOC PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 70 RUNT
RUNT 5 RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER : SVER , , Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH phDY big phQL c phTR phTR k#?`DuCdb{wMzmaiv5&oX ,zNH~h9bA3^%^#?p;ZJ}wXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]fuq.$>y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6z x:H-p@~KCozPk&OE6OLntr%3bbcy[*oCf!`LY&0AS_7eaPt;LOx,T2PYbWF`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJ AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT ` ` W( B1Pt B1Pt BCTS : BufT ( P \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ( P ^ DATA ( P DATA ( P DATA ( P N DATA #] F DATA
#] F + DATA #] F rI DATA #] F DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab I FVoc I FWO_ GATC Feat / / H GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ 91 LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD $ P2AM 0( P2BA 6. P2RL *" PBAS PBAS PCON
PCON
PDMF PDMF PLOC PLOC PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 70 RUNT
RUNT 5 RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER : SVER , , Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH phDY big phQL c phTR phTR