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()  & 1!!    #1- %#$*#"*$                            "  *(       ) ,!$ !#.'!!!         & $$0,+(+,'*-/7%6*9%,         +%!)!.' $  %. %#0%()1)#%65/%&)++)   #         # $ $(  ##     #                )%$                         ! 0 !             #       %                       !                              %        ~+                       "                             !              &"                    "         "          '                                  ' "                                                                                          &  $                                                                        P,,,III^^^{{&&&999DDDXXXaaavvv      ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRI!$%%%$$$#!   9[[RRRttt'((222==<GGGQQQ[ZZdddmmmvuv~}}<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 82395016b9ff474fae1eb4682fbbfe39Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_01-55_07433130Seq1248112481_Costion_K1322_matK_Fjmadera96-WellK1322_B4KB_3730_POP7_BDTv3.mobSeqPop7z"#!"$%%%$%'),./0112222222344433334431/-,-./0/.,++***)(''()))('%$$$$$$##$$%&&&%#""!!!""$%&&%#!   "%(+-..026:=ADHLRX`is~|zz{}!Bh#5=;0hE(zsonmmmlklot{ 0Z *:9'l:xmd[RLHIQa{ W] ?r?woic^ZX[f{C'<;&Qzh]WUUWXXWURNIC=6/)$!!$*3?LW`ed^UJ?4+$                                       8:;<<;98;BLYchgaYSQT[ckpsuuvwxyyyxwwwxyz{}~~}|{{{{yvrolkjjiihfda^[YXYZZZYWTRQPPPPPQRRSRQOLIFEDFINRUVTQLGEEGJLMLJGEEGJMOOMJFB@>=<;97779<>??=:87789::;<>@AA@=:75445678876431124689:;;::988888640-++.26:<=<<<<==?@ADFILPSVY[]`gp~"OCo4^|}rh_[[^chjmq{ 8oMg2a!s&~C\\ByHm tF't[I<2+&$%,9Lbz-Rp{dG&|{7JTVPA([@.#&4JhNI%&y_Vb!:{-}i^_l )Cax~ \l{5'u<5WspR,n]NB80+'$"                                                                               "&*-023468:<?ADGJOSX\`dgknqtuvvwy}{tnigghiihfb_\ZYYZ\^`aba^\ZXXYZZYVSPMKJJJJIHGFECB@>=;::;;<<<<:96432112222210/-,,,./111/.,,,-/012110/.--./02333334444431000123455432211110/..../01111223455432112579;;:989<@FLS[do|0b'z,!e$8@:,rL!f8 u_SS\n&Hn;xz<t4jT>%.G`x{iU@- "7YE q4{uu{^6dVYrd@tMF\{`F(wF4YLDaT^,xlaVNGB>;:<DQe~{eTG=4,%           !#$$#!                                                                                                          !%+4>KYj~ '>P]baZN=(vokkou} xmcYQJFCCCCDDFKR]kzobWOID@<976554320014<GUfx{j[M@6.)&')-/0/,)'''''%# "$%'*-1478751.+)))))('&$#"!!                                    %m@AEALA fKB.bcpKB_3730_POP7_BDTv3.mobtermAAAAATTTAAAAAGACAGCTCGGGTGCCCCCGCGCCCCCCGGAATTCCAACCCCCCCCTCTGGCATTAGGGAAGCTCTCTTGGGGAAGAATTCTTCCTTTTTTTTTTAAATTCTTTCCCAGAGTGGATTGTGGAAAGTATATTTTTGGATCAAATATGTTTCTTTTCTTTTAAAGAAAATATTTACTTTTTTCTTTTTTAGTAAAAAATTTAAAAAGACAGCTCGGGTGCCCCCGCGCCCCCCGGAATTCCAACCCCCCCCTCTGGCATTAGGGAAGCTCTCTTGGGGAAGAATTCTTCCTTTTTTTTTTAAATTCTTTCCCAGAGTGGATTGTGGAAAGTATATTTTTGGATCAAATATGTTTCTTTTCTTTTAAAGAAAATATTTACTTTTTTCTTTTTTAGTA 1AP\ju,;JQ\es} 0>Ldv%3?Wdn &/@T`v/<GYmx",;EQcz&1;FRbp (3AN^it    / ; G Q ] h r }    0 ; G U _ q ~   " / 1AP\ju,;JQ\es} 0>Ldv%3?Wdn &/@T`v/<GYmx",;EQcz&1;FRbp (3AN^it    / ; G Q ] h r }    0 ; G U _ q ~   " /                                     "                                            "       cMb$J- 2 ;*(@_0B(WV^[ ?7$c5\9/X10 #A$6F`YT EYVO3 7DG.A I %674 Y]/UL) GRI ,> F'R[ 5,-W D.*Q$ZH.T`"6S 5 $*9U"Q"X3] \>% )>WF\GM0^]Tc5RB_Ub\b/\[a"LMMK&\ :PZ1YrpPYV*9. 3 }?.32n e[k-RU2jxbo{ , 8luV/`j+' ?F uawLD(41^ Cg4yi+a.8+lh\1xh4/Zc!WGO>TE_ y&E)^d< #3~qi9~'_vrI"W-'>La }   (.YGE;I'*  j< <  "(],L,En2mekN?dKKYI,I}{;I%{>{ }3WkJ)ppLLM6.X 3d~Yvy-dPkaS+-E;?|z7qd(=]="* &sXh@ :+3/UDu](^h)%)1`/AgWrWr+^(f[,4RfE05x06(;{7pu9)\3] *~(,}t3Ij '`U9e"  0N~~ykD #? | DKl{5nZGGGTTAAATTTGGATATTAAAAATAATTTATAAAAANTTTTATCAATTTCATTTAAATCTCCCTTAATATTCCCTCTCTGGTTACGGATGCGTCCTTCAGGGCGAGAGGTCCTCCCTTAGATACATGAATGAAGTGAATATAAACCAAGATGTTGTCTCCGTCCCCTAAAATGGAGGGGGTAAGGGACCCCCTCCCGCATAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 03:26:47 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #GDATA #G,DATA #GtiDATA #G3DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ILIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISP1AM &P1RL P1WD P2AMP2BA~P2RLRPBASPBAS8PCON PCONPDMFPDMFPLOCdPLOCPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCH3phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #GDATA #G,DATA #GtiDATA #G3DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab#,FVoc#FWO_GATCFeat.."GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ILIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISP1AM &P1RL P1WD P2AMP2BA~P2RLRPBASPBAS8PCON PCONPDMFPDMFPLOCdPLOCPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCH3phDYbigphQLcphTRphTRc:]FN}T3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4O6TSN{4V[JfWQv3[p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRb 2)FB[yEZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=#LwXpFl+b{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H>R7R@n\\?8!hNnK%v1fp?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&03BSmv^Y6UziP-#=c$DfqcdsTF]luKgk_lpulAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtAUDT``1?B1PtB1PtBCTSBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #GDATA #G,DATA #GtiDATA #G3DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab#,FVoc#FWO_GATCFeat.."GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ILIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISP1AM &P1RL P1WD P2AMP2BA~P2RLRPBASPBAS8PCON PCONPDMFPDMFPLOCdPLOCPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCH3phDYbigphQLcphTRphTR