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P,,,III^^^{{&&&999DDDXXXaaavvv      ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRI!$%%%$$$#!   9[[RRRttt'((222==<GGGQQQ[ZZdddmmmvuv~}}<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c8910ab5cef24277ad95df43334816f2Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_01-55_07433130Seq1248112481_Costion_K1322_matK_Fjmadera96-WellK1322_A8KB_3730_POP7_BDTv3.mobSeqPop7z (5DS_jx ;^Kz)c;N> ) i > u 9 ?  : Z 4:- y W : &    x H  t M  > } *vX74HRPG;2+%k,OW9+eJ:/"Fw +DT^cefghgaU?kJ6,,3?Od|/a3DLD$Sc;n\J7 &=Xy =j 9wF{} <q7Pdt3}7GsxV.BS}7rkvX2n,>[t<E6TrM1 #/?Qbpy|xodYQMMQXamzmYG6)   #1@N]owQ6J8dqbdv"mO9+# %/=M[baYND==AHOV\adeb[PC5( $1CYn{m^PC8.%   cNh 9 JUl q ot{|pg_VKB;864/% .=FG?2# (/33,"   #'&  #)/:LhxS4 *,&   %0@TlhO8$ .=GJE=64;IZholaQ?0% 1RupO3 )6CQ`qm^Zcy !' {uoib[TNID?:66=Lb}v[A+  *9CHF=1"  +<KTRF40Uy}[5  .ZGXFD&DioM/ *8HXcgcXH9+#!&0>O]gjdXJ?;@L[jstm`QC6+"$-352+#     /U\8!  +C[p}{mYC/!*144/&   "*2894+"$*+&'4@HKHB:3.-,,)" %*(        !)29<:3+#"#!  !')%     './+$  !%&%#!"%+0440(      !"$&('"             &3@KRTNC3$+=MVUL>/$ !"!   *12.% !')%!,6;;5-#                             '/43,!                          $%" $3>B@8,  (+'      "%#       $+/0+#                       !&'"          #()'!            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"(/5:<;5,                                                 ')&     !                 &,+"                                    !$#  "#"         "+-'          !%$                                                                                                                                                                                                                @هA AIA {KB.bcpKB_3730_POP7_BDTv3.mobtermCGAAGGGGAAAGATCGCTGCTACTCTCGTCTATATACTTCGCCTTTCCTGTGTAATCCCCTCCTTGCCTCCTGCTCAAAATGGAGTAGATTGTAGGGTAAATATATCCCTAAATGTTTTGGGTTCTTTCTCGGAATGAAAATTGGAAGGGAAATTCCCAATTTTTTTTCCTCTTTCTACGACTATCGAAGGGGAAAGATCGCTGCTACTCTCGTCTATATACTTCGCCTTTCCTGTGTAATCCCCTCCTTGCCTCCTGCTCAAAATGGAGTAGATTGTAGGGTAAATATATCCCTAAATGTTTTGGGTTCTTTCTCGGAATGAAAATTGGAAGGGAAATTCCCAATTTTTTTTCCTCTTTCTACGACTAT +3AKR^hx(3<ITakx+3AJOYco%/;GRau%.8CP]ku 1@JSaq&2<FYas *5?LZju #/;FVbjx    ) 4 ? +3AKR^hx(3<ITakx+3AJOYco%/;GRau%.8CP]ku 1@JSaq&2<FYas *5?LZju #/;FVbjx    ) 4 ?            !+'              "                       !+'              "           C-L/BcRESHQBQJ87`H?NQV_= (,5,!'V3><"0$c>4(-JG0bQ]BM7?D,91'WIV9KW ?-LS 7(;?<5M43V0.3EM<aDYPTKF. "=&#C*N,\<_DXC[*U#,S:NR GT>.?:U< J 7 X\7I} q U]Xe( z  59++ r t^e^'#wOK Sn<W}t\100 !wF+Bl.c \ni@@[ x2!CN$[PskI#QZ\%  P" aH\ 4 GUxEF0:)+xHo_fCbJ^W 2P\LP<rMgYPm:D0MgU8/]OK DK R*QT{% (C_6FX@  I#s$ RRV DPpp rpr)W;p&"` 2|sLk5y_'BXmb>"*P/3p{Xw||?lQHM3> ^fQ\ijzCGCGGAATTGGGTGGTTAATTGTTGTGGTAGATGTATTCCTCGGCCGTTCTGAAGTAGGTTGTACCTTTCTTGCGCTTGGGGCCCCCTTCCCTAGAATGCTGAGGCCTTTCTTCGTGGGGCCTGCTCCCTCTCAGGAAGGCTAGTAGGAACGTTGCTTGTTGACCCGGCTTCTCAATCTACTAAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 03:27:21 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSlBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #cFDATA #cF+DATA #cFraDATA #cF'DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~ LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISP1AMrbP1RLrP1WDrP2AMr P2BA *P2RLr FPBASPBAS(PCON7PCON~PDMFPDMF PLOCr PLOCrPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER aSVER,,Scal@Scan3TUBEA8Tmpr<UserphARphCH#phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSlBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #cFDATA #cF+DATA #cFraDATA #cF'DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~ LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISP1AMrbP1RLrP1WDrP2AMr P2BA *P2RLr FPBASPBAS(PCON7PCON~PDMFPDMF PLOCr PLOCrPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER aSVER,,Scal@Scan3TUBEA8Tmpr<UserphARphCH#phDYbigphQLcphTRphTRmkYbCK)l_pD8Jq,./b _.uKxh6O1w^{l{iED>{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{MoW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{Z(cOH?3+]A:LPZ+($^UV>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E:.<)%,8EE.Om,P>bH6X128)TK7\4v3s'kY6C}B-mq5FaT2V3pVoqCH-p@v\vhsW__Z';fQGE-`x&iJ-,qzjN"o+Ax8amb,.63<%Ri24WF=-U[D~TWY+N@c'v+gK >o>s1H#"$w8@&!]T68n(0Ju*zv&1M2F{H/`WajZUTw|INi@C,6KP8)s5AVihx_7&OMb=#w&bN"@7hhSc%bVm? S8UXabCDkS4NRY0GbR31}ssb[l"G>}O!G,lwdRadJsV&1ZG|JW+eT`Vcyu3Be%{M*A#t5#r~^;?2p$MXAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtAUDT``+B1PtB1PtBCTSlBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #cFDATA #cF+DATA #cFraDATA #cF'DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~ LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOISP1AMrbP1RLrP1WDrP2AMr P2BA *P2RLr FPBASPBAS(PCON7PCON~PDMFPDMF PLOCr PLOCrPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER aSVER,,Scal@Scan3TUBEA8Tmpr<UserphARphCH#phDYbigphQLcphTRphTR