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[[[RRRttt (((232===HHHRQQ[[[dddnnmvvv~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 77a8f0c13b8e43238369bc783179fb98Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-18_21-23_07403130Seq1247812478_Costion_K1320_matK_Rjmadera96-Well K1320_D2_RKB_3730_POP7_BDTv3.mobSeqPop7z2FZp $Hs+=Oe9_!Fg #4?B=/a0zO#W"\.xk]OA4)!!',0220.-/146667;EUhz{}?f%;HKB.|?fB' )8ESct{bL=;DViw}~|}gF$teYQJFEFKRYbipuwxvspqv 8a[1r]NFBCFLT_m~qT:(!,Me@:zh[Q. 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(B]r|yiQ6(HjzY6 "?]vwbI0 !"$'-5CTfw}mXB.  .F[ikbQ:%  !8PdmhW?(1Nl|dH2*4KijL/    7UqybD)2SusN*!BhhG*       !6ITTI6!   -696-"   "9N\_UA)  $5EPQH7# 5JY]UE0  "6L^heWC,   )@S]\O;%    .CSYRA,"5ENL@.    .FYcaS?(     5NajgYC,   4PhwymW=%  "7KY]VG3 !-45/$ (.-% &;LSN?,!6JXZQ@+        (=P]_WF1                                                                                    }@A6A?bA"KB.bcpKB_3730_POP7_BDTv3.mobtermGAAAGTTTCAAGTGCAATCTGAGAAGAATGATTGATCGTAAATTAATAGTATTTAAGTAGGAAAAAATACATACCGGCAACAGATTTCCCAAAGAATACTCCTTTTTTAATATCTGACATTAATGCATCCACTTTCATTTTGAAAACTCTAGAAACTATGTCTGGACGATCTTCAGTTTTTTGGCCCGGAATGAATTGATCGTAAATTAACGTCATAAGAAAGTTTCAAGTGCAATCTGAGAAGAATGATTGATCGTAAATTAATAGTATTTAAGTAGGAAAAAATACATACCGGCAACAGATTTCCCAAAGAATACTCCTTTTTTAATATCTGACATTAATGCATCCACTTTCATTTTGAAAACTCTAGAAACTATGTCTGGACGATCTTCAGTTTTTTGGCCCGGAATGAATTGATCGTAAATTAACGTCATAA *8@QZbqx$2?HQaq~ #,49EPcny(1=JUan|  *6BMU`ju)3>GQ]gs} "-9EPYcnx&3>JS_js#-9DP[dp{    % 7 C N Y f s  *8@QZbqx$2?HQaq~ #,49EPcny(1=JUan|  *6BMU`ju)3>GQ]gs} "-9EPYcnx&3>JS_js#-9DP[dp{    % 7 C N Y f s    1$ + ; &1+  .1 ""331&";+);;>;3;;3;;;;;;866>>>>;+8;11+&13;33+;1"&+3);81+1"1322&3+13++"" ;;+13>>;+  ""'"""+3+3;;;+ +3;       1$ + ; &1+  .1 ""331&";+);;>;3;;3;;;;;;866>>>>;+8;11+&13;33+;1"&+3);81+1"1322&3+13++"" ;;+13>>;+  ""'"""+3+3;;;+ +3;    &P859_5WR9$SB^;6 :A!2 .Z A;;#/X)MUX,:) G cS%CV I.K1V1BD][6Xa 0TV+ LKLM7Ja^= AM]L@C0&G`CH#c'L!^&. #TY =/a ?5&La#QV<^F80:U-.*\"XTU@] BaD`-4XE=^/"#]4Q=F K (<`Z(A^0   A  j bEXm,|``Te#wk7:4\   XOcV>F+_,yL}5{Ve~~ >p)!D)I&P!pf :%A?Nn<4o/yF!M @ f t_6- [Ph#\YTM.Md2f  g ($\ht qJ\l~?BZpA),\&-YR3!C)HEsp P5 /), ^u;mdN,(Wo+soEdd$x{I;,,8Nd-,R8dCt?-@,_ 6)d*XeW8H-].8xg#!8`veS 6`zJ5piwWTv]wTx} S]^*+dOV/iB~[4LEa Wd;Wc1`}W|k@'1du}w>m\d~yFPoMGcOSOd_~_iyPg[#Fr }v\WNxwn}euUE>Vss}Mjob#;On!AGGGAAAAATCAAAATTAAAAGACCATTATTAGTTATTGTTTAATGAGAATACAGGAGGACGGGCGTATAGGTAGCCACTATATAACATACGCTTGAGAATTCCACCGGTCTGTCTTACGAGGAAGGCGACACCATGACAAACTGTAGACCTTTGTAGAAAACCAGAAGAAAAACAAAAAGGTAGGAACCTACCCACCTATTGCCTAATCTAAACCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-18 22:53:29 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS=BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #VFDATA #VF+GDATA #VFqDATA #VFDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISSP1AM P1RLVP1WD P2AM &P2BAP2RL rPBAS`PBASPCON|PCONPDMF{PDMFjPLOCPLOC:PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 5 RUNT ,9RUNT 3$Rate RunN""S/N%KSCAN3SMED SMLtSMPL pSPACMSPACcSPACMSVER4.0SVER 2SVER,,nScal@Scan3TUBED2Tmpr<UserphARphCHphDYbigphQLcphTRphTRAɁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS=BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #VFDATA #VF+GDATA #VFqDATA #VFDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..XGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISSP1AM P1RLVP1WD P2AM &P2BAP2RL rPBAS`PBASPCON|PCONPDMF{PDMFjPLOCPLOC:PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 5 RUNT ,9RUNT 3$Rate RunN""S/N%KSCAN3SMED SMLtSMPL pSPACMSPACcSPACMSVER4.0SVER 2SVER,,nScal@Scan3TUBED2Tmpr<UserphARphCHphDYbigphQLcphTRphTRV#\*x9*ofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72We/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LJZV>UB_fB(VCb(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#FmFiAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTdd-B1PtB1PtBCTS=BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #VFDATA #VF+GDATA #VFqDATA #VFDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..XGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISSP1AM P1RLVP1WD P2AM &P2BAP2RL rPBAS`PBASPCON|PCONPDMF{PDMFjPLOCPLOC:PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 5 RUNT ,9RUNT 3$Rate RunN""S/N%KSCAN3SMED SMLtSMPL pSPACMSPACcSPACMSVER4.0SVER 2SVER,,nScal@Scan3TUBED2Tmpr<UserphARphCHphDYbigphQLcphTRphTR