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" $ (%"%                            &!        .'#%      /'(++)! %#*)  %' &%) ## " +"# )           #'" !           & %            #               #                                                            %                                                                             '                                                                              d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRA$'''&&&$##!   [[[PRRttt))(333=>DHHQRR[[[eeennnwww~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 19e39b0bd07c49edb1abc3851eeccaf4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-18_18-21_07383130Seq1247712477_Costion_K1320_matK_Fjmadera96-Well K1320_B12KB_3730_POP7_BDTv3.mobSeqPop7z=?BFIJHDA@CJT]ejmqv~#/;J]s (Gg&Gm@t'JjS_'h<xBui[K<-!(7HYguAbrijoqk[D+!6Pm%qaP-      |o & A 9 tjtQ=45@Y1n ?qm)Fo0uppszxog_WQLF@:2,('''&"")-.03:ETbmrpi`VMF@;61.-.29AKVbmw}|ulc[VSQQPNMLLLMMKF@8/&  *4<?@>;8520.-./148<@DINRTTROKGEEHKPSUSOJFDFJPVXVOG=6225:>?;4* !$(0<L`tiP8$    <k4t{D |aOA7/**/;K\lx s`QE=9789999:<>BGKPTVVRMJKUicF.  !$&'$  7`uiQ > fi@DMB -Hczm[LB=;:9852004>MayuV7  !&))(&#"   &/452*!  )020+$  !##   &=Zxte^_ceaTB,   )142*!  !%&&%""$%#  !"#$$$#        #$!  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""!                 ,7=<5*             (..&                     $+.) -;A@9/%         %,01101121.(                 $'&" +8@A<2(    "%&% ",49;951,(#                                     #**$ '6?@9,                                                                                                                                                "!                 %19<;;>GVgy&Fg,_0cL .NkvcJ+|j[MC><=@BGO]t?l-EYlrX5 aJ8) &Fo%OwTP_u  5 <  $. K t n L " O S@)[x~mJj7 b> <s?VZJ'w7cK7)$+<Xz3Nag^D~N*+A`3mkZYj5EA'w,j? %Dl3Zuyb>mR=-"  0Fb4]z}_0U1 .U9gxNK 4c-pg-1lq.zT:*  !#" '-0/+# :s$g+RqNU1#s<#,26=Ps?t/5Yd:O) 9r f C & M(^=\Tp9     (-0/-("%.585,     ,@Wlz~vcK3 &0661) #+15751+$  $*..)   -TxM* !$%#  %&$  #(++)&%%')**($!"%&%!        "# !%$                     &**#     (3:80#        !!#'(&        !&&"$&$    !).0/*"   %'$       #!      ""         "$"                             "()% !(+)#        ",4:<92(                                                                                                                                                                    &*+*'!                                                                                                            !"%)/5:=>>?BHNTZ_bcehnx5Vy -Qu?k .Pp.CQYZWTT[hxp\G4%-C_|oY<~F ^(0\F`i`I&Xu&yZB, .T{6f'BYjuywl[F/,690lH h=zyz|{zxwxz}~|vmcYQJE>6.&! $*2?RmUSp]Xax2a%@OV[f{!@J;lJth_ZXWWUPH>3+&$&*3D`S(+r,cVT[dkkdWE4%   !)17;:5+!'19=:3* 9i2pw<X9(!"'/7>DFD?8/)%&)-/+%(CqAs7pT?/$  )HwET \<*"'/7=BFHIGB;1' >lKa #d,    '-/-& %3<=7, '7IZhnmdUA.#)+,-.28>BCA<6.&    $((&     *:GLI>/  #%&#   ).0-(!  &1<DIHB;4/.14652.)''*/47860(        %+.-("      #&&"       "#$$! $$"   %%  $,120,&  "$$#"#$&&&$                   $)(! "),*$                                                                                                                                                                                                                                                                                                            jk@A|AROcW ZU*2/552@TRX5/552/2%6< !+#b(, E &\2? (<(P(<(<](2<P<QG\&VS`/(@<(<(G"Ba@H.2VE2(< qr<Y Ek"U8\(i2MlVFa`aE+ 1$*nzDN$Pg}F Q$1V L( +t-b+.c~8VT0E.N8U=O6D2*_ J9;.o 8 (c)2nOXEGe }WF:UX4ylVqPk:L D%x>_mJ 7R  !X%v0|5Ih!ntTS mDoAFQejq03V\R.:P_; & + (pd8pppz%hVir{!6t)oyQC_ !/B=m,+rHlbT #38'1yl/<VV*X{[$AqKe1h3x@pFJ4z"b%ns8%& xEk 49|P!(A u'0  ~V x{(_[`a8 ![bsH>!<!lKG b+34  > ][guZjnxKbAn,F(" eIccSBB1o]nd<RB;F9)>RIpI)CUWyehv#F@+z.RRpNKeHsQz|'Zl`p H`C?+d&CACATTGGTAAATATATCGTCAACATATATGGCGCCGTAATACGAATGAGGACGTTAGAGATCTGTATAATGATTAAGTCCCTCGGAAATTAAAAGAGGATCTCCTTTCCTCGATGAATGGGTCACACACCAGTTGTCATGATTAGAAGGTCCCTACCAAAGCAACGCGGAAAGTTAAAATCCCCTAGGAATAAACGCCCGACTATAGCGCCTCCAAGACACCAAACAACAGTGGGCCCCTACAAGCAAGCTTCAATTTCTCCCCTTCAACTTTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-18 19:52:59 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSwBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #6FlDATA #6Fl+DATA #6FlqrDATA #6FlDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISRP1AM& CP1RL&P1WD& iP2AM&P2BAP2RL& PBASPBASPCON PCONPDMFzPDMFiPLOC&PLOC&PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 3RUNT + RUNT 11Rate RunN""S/N%JSCAN3SMED SMLtSMPL pSPACMSPACbSPACMSVER4.0SVER lSVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSwBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #6FlDATA #6Fl+DATA #6FlqrDATA #6FlDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab"FVoc"FWO_GATCFeat.."GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISRP1AM& CP1RL&P1WD& iP2AM&P2BAP2RL& PBASPBASPCON PCONPDMFzPDMFiPLOC&PLOC&PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 3RUNT + RUNT 11Rate RunN""S/N%JSCAN3SMED SMLtSMPL pSPACMSPACbSPACMSVER4.0SVER lSVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCHphDYbigphQLcphTRphTR0ZNch>st_@U])%fD1,;0{U[%~#mXYV<{#-$>]/mU>p>X|.8As*5.@h23|=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbNfPZ2V~oLSHmR/+3p+M*JgbUA6WJ?eee1K/kx=gc1/kdSPaH~Cd*9+cUV&=o?eUKGM5J_32&L"gr[&Xo-,(B~bjOe:=fQSqMM`^|*7GZk'hTb1IZ@V$^(R[mPo1Cd}FxQPw#vpkMSvYTEl^Z-I-{0vTNiBx["#\+h!cS/'9lv+@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGUNM(pz)2(lM=(Q)jA[NjTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9Ul=#Sw,vcVP 5Go-D7I 9xxsWD@4ir$GE1Xz.: