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d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR A $'''&&&$##!
[ [ [ P R R t t t ))(333=>DHHQRR[[[eeennnwww~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5535ccfb7bca408fabf605546ad5aed8Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-18_18-21_0738 3130Seq 12477 12477_Costion_K1320_matK_F jmadera 96-Well K1320_A6KB_3730_POP7_BDTv3.mobSeqPop7zA* C ecvK4AhI ;pRID1 `taNti:Ou BC w
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"PN63r\!bC P@j(L'g k0z.:MI wt cA <c(GAGGTTTTTAGTATAAGATAAATCTTTGTCAAGTAGCCCGTCCCTTTCCCTTGCAAGCCTCAATGGGAGACGTCCCCTAGAAAAGGGACGTTCTCCTTCCGGATGAGAAAGACCTTGGAATTCTAGGGGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-18 19:52:15 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt | ASPt | B1Pt { B1Pt { BCTS S BufT " D \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D Z DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~
LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS OfSc v P1AM 8 P1RL 4 P1WD < P2AM D P2BA
H P2RL @ PBAS PBAS $ PCON PCON 0 PDMF PDMF PLOC , PLOC ( PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER H SVER , , Satd ^ Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt | ASPt | B1Pt { B1Pt { BCTS S BufT " D \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D Z DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . n GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~
LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS OfSc v P1AM 8 P1RL 4 P1WD < P2AM D P2BA
H P2RL @ PBAS PBAS $ PCON PCON 0 PDMF PDMF PLOC , PLOC ( PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER H SVER , , Satd ^ Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH phDY big phQL c phTR phTR ]%A'on!w.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwN1:^MU9GUsg[TbOr(Iu]AGc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqiH(IM_C1zwu+}2o~*,p""#]eq }X'KD7I 9_[*?bCDkSOj!Q4M"}JMzR9JK[Yl)d762H#A#Q9U,Z:X=<;q4n%DSi^tpuze2#Xxiy?,1OPh~wA[h@tC]"#]}l~e,SXtmI^#lW4?/D:Q64Pot pN9IlVOY(xs/b@j/.=y`6@{u4f\TP/h;ZQkwqzFGP7T]V%R5;MmN2CgGz^nLoT.iPtj glX ,cW`e;E;qK|-Vz^b;0|>kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?WQjHb(TZ#qpG(>1"#Y7!OkWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8fQI1qs9;[1jk[K2;+hj3@*7_62Q$OEH5 G,90lVSFsA bFX97z?&q6X+9mK\TI_aEdO{7+VDTj.uFPqWgZ*me2&dAr&;t[W?*b1j glX ,cW`$~+hj:gh_)!hs|O1^M6C,nJ3qo8-@MV^A$+v/y#PzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=KubQ2a50jj,4o-8N'nsv&'Y?sxWlZN.YHng=! AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt | ASPt | AUDT ` ` * B1Pt { B1Pt { BCTS S BufT " D \ CTID + CTNM 1 CTOw L CTTL T CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D Z DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN 5 Dye# DyeN @ DyeN E DyeN J DyeN O DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . n GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~
LIMS ! ! ] LNTD 2 LsrP a MCHN MODF ~ MODL 3730 NAVG NLNE ` NOIS OfSc v P1AM 8 P1RL 4 P1WD < P2AM D P2BA
H P2RL @ PBAS PBAS $ PCON PCON 0 PDMF PDMF PLOC , PLOC ( PSZE ` PTYP T PXLB RGNm # RMXV 1 RMdN
RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER H SVER , , Satd ^ Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH phDY big phQL c phTR phTR