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(/#                                            /(%/               !  !! $             #           (,,,III^^^{{{&&&999DDNXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR!$&%%%$##"   [[[RRStt(((333=>=HHHRRR\[\eelnnwww<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 92f0808e69854a47823e952b5b68ac39Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-18_16-50_07373130Seq1247612476_Costion_K1319_matK_Rjmadera96-Well K1319_H9_RKB_3730_POP7_BDTv3.mobSeqPop7z&\~leu/yK}~K_ = z d U (":$F&e(*-^/14<6}8:= >@oACEoGJ8LNP5QQKQ:QPPiOO[NMLKJI:GECAq?j=<:988&7M6^5P4&210/t.w-,s+:)(& #!=~9ms.i$,QqP N]l@_)nq!d .  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'$@IS>@0QA!&" ,PQ5Dc4AKVN YQV ,FB/ G PJX2[3']X-NN :: F/+GP \?RL 5RZ?<),0 L/K c O0Q(M4/[A",JH#-[0(/O0[:%`7: !V#KI"`[M88G8 +R0!\KI,[W U71E3+$ F:L6 1>?NR.YZ)K(^b`R*A;PN]'H?JWD;:$8-$KC 2 \`H)A" AA1CC$T3#YC?'F`$b*]U;E$> ZW7B b]6'M `0W4_2''+/(QJ_1#^, WdAZBF c01J\bM%&A19GF+7ZNU;)Y]C2U2Y>74P/2V55/5/"]bcG'5\E/]V(i2 F'  _!FZ! 4u;B4 Fzn} a~><x)zj 1tB?ko  t/dF Y1#{ZAa]g>nnG0t 99L,Yns1%</%fUej{bl J]\0E )x8~ndgPNMecY54TbHJzJL|bdZV&`Hj>Jlb.( >@t14<5S|D-?whfQ8!m>/5}Wt%c&q|?{  Ux<(xv{Ugta/3W.W($u&+ |_Ux`' 2L8P?d%} }5xua uA7_'d s WuI<C,}<6db_*w8j5U*  (-$~JN5:R:C J2d#%Rg5<N6Mb'k&B`J#}/i9(%*iMZ e Y"<o,%8/  ,=:H@8 5 tx  Xo  `)QI'E8*/K2bL  9: $+:!5T8 OZ /(   &$'%*=47$;EH("*@2Be   h AGa c>R9X)<Me W/`v8 ]YU&Rfk$2)9,+{93$6.@S!6y%Vo|5Ee( F:]qf`{fr"Ll-- ---2f{|p 0dKm<HIlDOFu\Z*dn QXXt{i*IPEp ?E)3qD{N"/ ]@%3E4Ix|VN {3h^e%4 X.{J.Mot,ee]m|pGXA$kFt@8=#pGcGB-E<Kp::0-n7I42pcdS(`OlTps|JB \1`iXt.Vtpgt \Zo\pPITKCp.y,Xx8M4XSOd^sj Kw% hK.+id'3, ?I3V y`*qdHB,5J.l<<Q[c`_&`"l/^3$ B_x`v]f'K5&Jx*[3@"Qxs9uPK=?}6|wZ nJYV 9 s92p+(a3,hz@{6X}X(pRE G$+fDQXy)>>0TGS6#^%P2t=M_Y*a{q0wr04V_d480ng.1|>u Xr/!uFnz?{}$/qKE*4mc@7K5wBM^0j^A`)@H5)7m%5 GGAAAAAAAAATTTATTAAATAAAATTCGNGTAGGTAGAGAATGATAGTTCTGTATCGGAACAAACACCCACAGGTTCCCGTCTAATTCGGGCCGATTATTTAATCGGAGCCGGAAGGATATCACTAAGGGATACGAGTGTAAGCATCTTGCCTCGGGGGAGAGCGTGCGACCCAGGTAAGGGTGGCATGGAGGATCGGAGATCGAATGATGCGTCCTCGAACGATGGGGGCATCCCCCAACCGGGGGTCGGAGCCCTATGAAGCTCTAAGTAATCCTTTTGCAGCCACCCTATGTTGCCGAGTGGAGGTGGGGCCTGATGCCTTCGAGCCAACCCATCTGGAAGCCATCCATGGCAACTTTGTAAATGACTAACCGGGAGAGAGGTAATCCTCGAATCTCACGATTAACCGTTTTAGTCATTCAAAACGTCATAGTAGATGCCCTTAGTTATATAAGATATGGCACAAGTTCCTCATCCTACCACTCAACAATTAGTGGGAGTTCTCTTCATATCTTGCTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-18 18:21:56 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS}gBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA .\DATA .\@DATA .\DATA .\DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~{LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISTP1AM iP1RL epP1WD mP2AM uP2BA  yP2RL qPBAS  W*PBAS  U PCON  cfPCON  a\PDMFPDMFUPLOC ]HPLOC Y4PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT ,RUNT  RUNT Rate RunN""S/N%TSCAN3SMED SMLtSMPL SPACMSPACTSPACMSVER4.0SVER }\SVER,,Scal@Scan3TUBEH9Tmpr<UserphARphCHUphDYbigphQLcphTRphTRoThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS}gBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA .\DATA .\@DATA .\DATA .\DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~{LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISTP1AM iP1RL epP1WD mP2AM uP2BA  yP2RL qPBAS  W*PBAS  U PCON  cfPCON  a\PDMFPDMFUPLOC ]HPLOC Y4PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT ,RUNT  RUNT Rate RunN""S/N%TSCAN3SMED SMLtSMPL SPACMSPACTSPACMSVER4.0SVER }\SVER,,Scal@Scan3TUBEH9Tmpr<UserphARphCHUphDYbigphQLcphTRphTReih4qH4l|t2#OoV$ zrTbZ?a23|jyHAj}W2yv!)QBmO7]N=]TG4/CeG1{?{sb: h,`,l6IEUY[^wA[h@t#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`K\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nvP?QYMA67E |VzM:*]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&'n[Nn%9SXWl573!dX!%~AfzvrfzoB=SWZM>U?uf8:jHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"BV_@iD]xy"^O[,z^E$6d+XnhcjPGh{;qeXd9U|q0D7$d(2!o,E-?){mnjH[q?Eb,8#V>AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VB!d+:pZ5KRCmnlcEn=6;}S$X:VDSBrRL8~f)NK1EDE?]wJUo6X?/WZ#6E]bZ&[';(Y`[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKST/4f_7}?e_MBSmv^N6T.iUltNR;wH#>TWQU"zi(V?go(r;4QV/fcd]R03^jQJM~j3XV]'DZNuOUf?eKkE=+4[PngD??Vc;6,A;{IF}oIiM%.zP{?7.N{C]*KWeq }X'(H;E}&mxXYnuPQh^*w% e MfaqQl'[)VBL$7V5*o&w+\kf*>Pyr{;qe&Ker"&O93s'!byH:/U?vwvUQ>1)oo/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@