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V F C > K I 2 p D U B c R T U H E X M K G Z A b W b U U S T h O ` j H X } [ S X U N ` ` r S [ u b f U W e l O J M Z E L M U F Q Q [ Y X N f U L Y \ I D C W l Y Y ^ ^ [ ^ Q _ Q T 3 L f P c D E 6 5 R F > a f Z R C F P C B 8 ? ; ? < 6 < ; > 2 2 : A 8 7 G 5 > H K < D ? 0 O N , 5 L " E B D ( ? 9 G < X P T L Y < H ? 0 B D G W T k a U S D F S H L E b X l V Y g k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b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e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j H / > K A ? * J Q B I m V : K X @ D : 7 ; > B " 6 B : 9 * Q 4 J J > S F ` O C D 2 I 3 A @ 4 G O : % 2 * 4 Q B @ J > T : T @ B F : 7 = L E B : 4 H I - 0 - B < 6 A ? C P P J - 2 @ H E F < > P H V C @ B 9 D 8 1 " 7 J 5 9 K E X \ Q L = C W 2 S > K L K B X M , 6 ? E : . , E Q ; 3 M 6 Q F H > C E 5 R 0 # 5 , ) . . ( R : F F = ? I Q 2 ) 8 2 6 , ) : + Q W : Y `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( 4 4 I 7 9 & = G Q J ) F 4 Q G 4 E ? a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` ` C @ M P D ^ F A B L A ? 8 0 G : 1 ) # G G @ A 1 O G > ; [ > Q 1 K 0 ( : . K C 8 ? 5 = J = A A = W . ; 8 ( ? / = E 3 4 0 7 % 8 M = , $ = 8 7 5 4 < 9 > D ? ' 9 J 2 ? 1 ' % 8 - N 1 D 7 E > - 2 ! ; ) $ 6 K 5 % @ H , 7 . L 2 6 N - D @ : 9 @ E @ D $ M K - A * ; @ J @ 5 ' 5 @ 9 L = B # , / 6 * - - 3 5 5 7 / 8 ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6109a381e43a47eb85695d35464a4d89Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-18_15-19_0736 3130Seq 12475 12475_Costion_K1319_matK_F jmadera 96-Well K1319_F5KB_3730_POP7_BDTv3.mobSeqPop7z L hx? y-_op?!f
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KB 1.4.1.82018-05-18 16:50:24 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
4 P2BA > P2RL
* PBAS PBAS PCON PCON PDMF y PDMF PLOC
PLOC
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT 1$ RUNT ) RUNT 0 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab & FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
4 P2BA > P2RL
* PBAS PBAS PCON PCON PDMF y PDMF PLOC
PLOC
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT 1$ RUNT ) RUNT 0 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR -&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{@2Km,1sE+4l{+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a! P2RL
* PBAS PBAS PCON PCON PDMF y PDMF PLOC
PLOC
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT 1$ RUNT ) RUNT 0 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR