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Z [ Z R R k t t (('222===GGGQQQZZaddmmmvvv~~} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e3a61461c0d24fc1b64ea60a5280421eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-14_17-06_0691 3130Seq 12413 12413_Costion_K1318_matK_R jmadera 96-Well
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TTTAAATACTATTATTTACACCTCAATTAAAGAGATATGCGGTATTATTAAGAGGGATGGCTACACACAGGCGGACTTAGCCGATTATTATAACAGTAGGAGGGGGATCTGGACACGGCTCCACAATTGTAATTAAAAAAAACGACACCTTACCTTTCAATTGGCGGAGCAACTATTTGATGGGGGGGTCGGGGGACACTCACCGTACTCGATACAACCCCCTCCCCCGAGTAAGCAAACGGGGGGGGGACAAAACACAACAAAGACGTGCCGCCTCTCACTCCCCGTCTTTCTTACCTTTTCTTTATTTGGTGGCGAAAACATCTCCTCCCCTATACTCCTAGATGGCTTTGTTATACCTCCGGTATTCACCTTCCCATGGACATTGCAGTGTAAATTCCGGTTCATCATACGGCGTAACCAAGTCGTCCTTACACCAGTTGCAACCTTAGTTATATTCGCCATCATGGCTTTGTCTCTCCCATGGACCTATATATATAATTTCAAAAATTTTACTCAACTACCCCGGCAGCATAAGCCCTAACCCTCTTGAACTATAGAGTCTTTACTCTTACCAACTAGGGATCTCGGAGTCTATAAGGATATTATTTGTGAGAAGAGTTGCTTTGTCGATTTCCCTAATTAGTATTATGCCTCCAACCTTTTAAATTTGGTTATTGATAATAATGAGCCAACTATTAGCTACTGCCCATGGCTAGTAAAAATTTTTTTATGAGGAAGTATTCTCGGCACAATTTAAAATGCTTCCTAGTACCCAGTTGTTCACAAGAGCGGCTAAGCCTTCTCATAAGGTTATTGCGAGATTAAAAAGCACAACCTCTCGCAAATTCTCACAGGCAATTGACGGTTAAGAAGTTTATCTACCAGATACTTTCAGTTATGGGAATAGGGTCCCGTGCAAGATTATTGCGCAGATATATTGCCTTATTGGCACACATTTCTAACTCCTTAACTAAGTGTAGAGCAGAATTGCTGTTTGTACTTCTCTCCTCTCGTTCTCTGTTGGGCCGGTAAGGGTTGGTAGTGGGAGGGGGGGGCGGTAAATAAAGTATCTCAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-14 18:37:31 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ρ BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA 3$ fH DATA
3$ fH J DATA 3$ fH + DATA 3$ fH s DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE C LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS } P1AM 6 l P1RL 6 l P1WD 6 l ~ P2AM 6 l V P2BA 6 6 P2RL 6 l PBAS 6 6 , PBAS 6 6 } PCON 6 6 p PCON 6 6 : PDMF { PDMF } PLOC 6 l PLOC 6 l b PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% } SCAN 3 SMED
SMLt SMPL p SPAC AZ SPAC } SPAC AZ SVER 4.0 SVER v SVER , , n Scal @ Scan 3 TUBE G9 Tmpr < User phAR phCH } phDY big phQL c phTR phTR x This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ρ BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA 3$ fH DATA
3$ fH J DATA 3$ fH + DATA 3$ fH s DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / ݜ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE C LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS } P1AM 6 l P1RL 6 l P1WD 6 l ~ P2AM 6 l V P2BA 6 6 P2RL 6 l PBAS 6 6 , PBAS 6 6 } PCON 6 6 p PCON 6 6 : PDMF { PDMF } PLOC 6 l PLOC 6 l b PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% } SCAN 3 SMED
SMLt SMPL p SPAC AZ SPAC } SPAC AZ SVER 4.0 SVER v SVER , , n Scal @ Scan 3 TUBE G9 Tmpr < User phAR phCH } phDY big phQL c phTR phTR A08^#5EN^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]^>\,FL$&\=3Uz=}kypy+:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYXS]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0SbXW,VE+,[Ol% eIe]fQp$rb=%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,Z:X=<;3On&+VDyK{t61h67A"yV+XZ