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Z [ Z R R k t t (('222===GGGQQQZZaddmmmvvv~~} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 209b5b3520dd470f837e1b73cab9d389Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-14_17-06_0691 3130Seq 12413 12413_Costion_K1318_matK_R jmadera 96-Well
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; pd i]kfu )SN. a$Ek +v7CATTTTTAACACCCAGGACGGGAGAATATGCGGTATTATTAAGGGGGATAACTGCGCATACACGCGCCGAGCTGGCCCTTATAACGGGATGCTATTTATCACAACCTGGACACATTCCCTTTAGGTGGTCCTCCGCGACACCGTAACTTAAAAGCGACCGCTATTCTCTCCGCACAATTGGTAGCACTGGTGTATTTCTCATAAATAGAGTAATCTACAAGAGCGAGGTAAGCTCTCACAGGAGGGGGCACGAGGGAGCGTTAAATATGCGCACTCGACAGATAGTTTTCCCTGCTTAGAACGAGCTCGAAGACGAGTAGAATACAGTGAGTACTGAAGCGAGACATCACATATTCTTAATTTCGTGTCCTTTGAACACGATGTTATAACTTCAAATACTTCTCCTGAGAAGTCTCTTCCGATACATCAGCAGATAAACTCCGTTATATCTCACATCGAACGTGGAGATATCGTAGGAGCAATATATTAGAGTAAGGGAAATATCATTCCGACACCTCCTACACAATATGAAATACCACTACCTAGGTATATAATCTCACCGGTTAGATTTCAGAATATGATTCTTGCCTAAGAGGATAGTTGATATAGACTTCGAGCGATATTGCCTTATACTCTCGCATACGAGTAATACAGAAGAACTTGGAGTATAGTTTAACTCAAAGTGCCCCTATAGCTAATTACCACAAGTAGAACGAGGTATCTACGCCTGATAAGATATATCACAAGAGTTTGACGCTATTAAGCGTGCTATAGGAGGAGATCTTCCAATCTACTGATCGCGGACCGTAGCTATAAGTACCTTCAGTATTGCAGTTAATGTACGAGCATATCTGAAACATAACTATTTACAGGATGTCGTTCCGCGGCCCTGCGCCGTTGAGTGAGAAGTGTTGAAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-14 18:37:26 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,g X DATA
,g X =i DATA ,g X 7 DATA ,g X DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ 0 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS G P1AM , k P1RL , d P1WD , s P2AM , n P2BA P2RL , zB PBAS K PBAS H PCON a( PCON ] PDMF { PDMF G PLOC , Vf PLOC , O: PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC G SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH H phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,g X DATA
,g X =i DATA ,g X 7 DATA ,g X DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ 0 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS G P1AM , k P1RL , d P1WD , s P2AM , n P2BA P2RL , zB PBAS K PBAS H PCON a( PCON ] PDMF { PDMF G PLOC , Vf PLOC , O: PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC G SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH H phDY big phQL c phTR phTR aB$T9F_z"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|*921NgChz)rI`Mvlb0HF8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH:/U?&wlDQ>1/o$/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]wclQ[>A{%i/"E.Om,\eyDW"[Fw.e{$AXj:F]W.JhTyE45l]hl5cS_d.u?zAw3+fF<7:cFU.F4/~L][U_1(_CcS{[}7"=I).zP{?7.N{Cj_"@?j~J!Eb4;,}E -kb+N4+t457fc|+{Wj2oFtX>j3XV]'p8NpdNmbcn3?GY\U {%KTfx'H9Kx'!byXYhRwZj1& evr.FZH~1:Ws?cP9"MV`+MTk0WC.%o,n`!3CS]n?KGxDIBG(XX7&ZoMQR,9hL AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT d d ' B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,g X DATA
,g X =i DATA ,g X 7 DATA ,g X DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE = LAST ~ ~ 0 LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS G P1AM , k P1RL , d P1WD , s P2AM , n P2BA P2RL , zB PBAS K PBAS H PCON a( PCON ] PDMF { PDMF G PLOC , Vf PLOC , O: PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% G SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC G SPAC AW SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B7 Tmpr < User phAR phCH H phDY big phQL c phTR phTR