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W h S B a O E U L F D ; j : P G B C I F Q C V P V G 5 Y D D E K N F I J / S D F C \ M \ V d E Y W V X _ O ? V D W N A @ M L J @ B L E A L J = K P R T D L J 9 O H P W S G M N H H O S 6 L E E U = H G N * H I I H 5 D H W E C ? M V T U M T J = R E G R Y F T \ Y J N e Q X D W W S Z L L M i O ^ Q Z ` _ ` [ N b V P e S D R X \ O [ H P R A N P P > N E ? ? @ c ? O K O V H ; K V H I ? Q P P K < F C I 7 D 2 B W J A H P V Q O Q Q T W W o _ Q ] S M [ W X O M M O N \ F Y I ` Z X J 6 = D I H = R X ? R [ Q d V b O ^ Y M S T N V P P F R C P J Y W a F b K Z S I = N g J R J ` d o p f e R ` a b H e b n J R Z \ C X V U E C U V V O L ^ G @ > N K O V S c ] W K ^ g X _ b R S g N H Q Y Y L \ I ` F X R N K O E c U T ] Z j _ b f h ^ m f r h ^ ] L R H c I H [ b ] N m b ^ b s ^ U P U H O P R V R c m l \ e s c j ` X T X ^ N O T O R I E O Y G E R M U T _ I X g c ` ` ^ u b [ ^ h a h T l Y Q V O T O M _ T J T H \ a Y V [ f R ] \ \ g [ k c Z _ X \ e f l S V U R T \ X _ \ L Z [ B L [ _ @ R e \ c ^ M B H \ ^ T G Z N l X L Y ] l S [ Y 8 F L K Q X D H S H Q X b c ^ f m d p h g ] X J R P \ @ B R ] e V ] l [ s g W \ = S S Y X R _ c T _ T i p s _ p i \ Z p \ k Z g q q \ _ y Z j Y _ b [ ` V j f T Y H ` _ e S b U Y \ Y _ L N J T O P L a T Q T U E L H R Z T U ] j m g h b e b s ` J W _ \ y U e i e h i W k a i P Z q d [ a d f Q f u X a _ ^ j d _ g b Z d k X d [ Y S [ G K Q I T N h W b ] ^ _ N ^ H s j d ` Z Y ^ X a N R U Y > R R V ^ i H F Q _ S [ P Y = L F 8 B = G G O : ? 7 M B W C 8 S G I Q ^ L S Y K O c D O A J H G N E W Q X O [ ] U [ O Y g M J i X V G Q K C R O F S N d P N X Y F U O K H K ? > U = H - X G E 4 G M 7 F K M M ; G : ? ; 0 / 8 A B = K @ I J @ ; < B J 1 ; = A D = B E + 2 I 8 ? N = L : 4 G : K Q L 9 2 A H ? Q < 9 B ? > . V 7 : @ A R D A E C < I G T C 3 E < ' G ? 9 < D / ? A > F N E : @ 7 A 7 A J X E < C ] M ? B A 2 8 X Q K 4 A C ? D 2 1 K F C I L > F : I M B F E N D I M I K I F E C O W f K U I Z T O O G N Z Q W 9 T _ P B \ T C @ F P H I B 9 G N ? F G U C Q R O T I M : G F S Q Z H = : I R H Z L E J @ U I N O J D O B 4 C U M O D L W c S X m b ` s n Q ] _ a Z L ` _ I N ` T _ R _ T X c K Q E H L L C = L U [ E U ^ P K Q I M N E A B d V H \ E H T I Q : D C K O G K W B R O M R Y F T [ [ I R J \ V _ E G T K [ R X O Q T T \ [ S ^ Y P U Q V i ^ X k a c M T g W N U ` : [ A C M H U L B B @ H E D G U H T F D E Q M M V X U S S Q ` V P M V T [ B K V F ; < I : O P A M N E R K Y K Y I G ^ S U c C T T D Z V P \ W M M E L E E M < H H M Q @ G K K L E H Y N : J @ H _ @ O > L F c H W _ H \ _ N S J E Y D F A H U B : X J B Q Y N S N J Q G D X F I I G Y F \ L F T T d J V ] a ` \ I K R h T a _ O R O Y L B X = S = X K @ = ; @ = R F T I L P X M O W W S J I h ] Y O G K X f \ P d R M _ P A P d P S P V V E 3 K 6 H F G E V ; B C A J P < S I R N V B A B J J M K J U K T L D M 1 O N N E F D O @ H > ? O V W [ ^ Y V V K e W ^ Z B W V V L K O O S O N T U _ I O U K H U L C Q S O V S _ W S V O N H N h a V G R s = Q ? G h [ E d M V \ K W C O > V = B R U < T A I N @ O @ J O Y O G S B L B F P O K M C J O > O U V N O O O N F J P A M P B S P Y I a Q ] e ` b ^ Y H j i \ e T O ? W J W V f ^ Y ^ X e S G ` d e h l Y a Z m e i e r i z q c ` i n X t K ] @ X C I O Z S S _ Q U [ L L S W f Z V o D T [ O U T W U P R U K X \ e O [ P \ R Z X \ U U o D r i F Z \ N J Q P e Q X a k P W b ` [ W h \ X H W M V y K G D L Q F E G Q > O L ^ J \ [ Y Q _ P S N D ] ] _ N R @ D Q M \ G V O F D Q H 4 A : Q M Y N W ? V F D : = E 4 B L D : : S D P L G H D 8 E 8 J = 8 D H N W B W \ I D Q O F W ^ P G f R W E ` T U Y [ J ` X K U X @ M ; H ; J 9 A ? ? D < L A F D I 7 F < K 3 C C N E J I J 1 J K C N > L I < E > V D T U O P T Q F < B S B N C L 5 < < J I A < D M c T a F W Y [ Z J F S N = R > Q O ? H G C C 7 6 I D = @ : 9 @ < > I A R ; C H K I f : L E U E U P J O f B O U ; N Z G d B O P \ R c c U W V K U ` N \ T K H Q P ^ Z \ ^ c I f a Y ] P f Y M b D Q ^ X L R H \ K B R Y N C A S B G D Q 1 E @ ? < D U M T H K L O @ U K C ? K . N K @ O M ` O W D D K M B B G R M I G P \ ? O K N H F H V T E g W b ` [ a ^ Q T W c Y P b T @ Z U K X Y ^ d ] X a j x f b e [ ] a S X [ ` ^ e V Y S a Z o m N X > H W ^ L R ] _ ; J O M R M M D < \ e k \ W [ Y w N T i W L P K N ? T W ] ` m k ^ R W X S Z W M \ ] X g \ ^ Q e M _ W N f ] _ \ = Q > B \ : U V X c ^ [ _ Z Z u Y V ^ o Y s X v j t j | l j b c W t b ` p [ i s W k f T d U W h i J Y e X g b Y N R \ Q q Y O n e S R f f ^ i U T \ G J \ B E = H O U ^ V a O L G ` \ ^ X [ ` O P S x Y L X I W d ? U R ? G E H R R D 8 J E G L @ E ^ M X M R P g M L a ` \ b \ \ h _ g l l U [ c b i ] Y f h v l g ^ b ] ] [ V Q f r L e n R N V W Q S Z ` C U V g Z H V Z F \ _ T S N < F C @ F C F B Q C G B R D K Q A C ? : J P R Y G J V X G X G D N K @ I 4 8 @ W S A T K < 3 H H O ] > E L ? 4 V T D O S ^ U a [ b E P S W _ F < D A M @ J M P B > ? J D 6 K S @ 8 M > ^ N ] M S ^ [ T R K X [ Z F Q T O c \ h Y R Q O ` U M a R Q Y k ` I L A R G > V 0 8 P 8 = @ ; D T : I 0 1 I G C K 9 P J R D B O 2 K M X H D Q G : F ; a H 5 ] T E R R I R W I ? 9 I 8 4 M O E B K 9 K M F K 9 E J 9 J 9 H 9 H Y U 5 6 J G > L J > @ @ G 6 E K G M H 7 Q G K H I A G V W O J ] a F G S \ S F D X T L E G L J M M E Q P G C Z O L C R X K M G L J A N K L K R K F P A W N L [ J : L I K Q P \ T E D [ N Z R K f D W S _ _ E I L @ C Q Q 7 < S 9 O H H N E < H V F [ M 9 Q ] J V ? I Z J S < A B N ^ N 9 U \ D M R R W A F 8 D M @ D D M D G D 4 K < H M P J E 7 F B 3 L [ ` M ] V \ S T W K S H E j \ P K ] T Z Y X U _ S k ] f d j [ ` b c ` ^ d _ g j ] ` W T c ] O a S T W W Y H Q J G V F R I P ] Y H e P O ] f Y N N [ _ ^ Q Q D E Y ; @ H H G 9 E I E L I D O U T ? N E F S 9 < < G @ 8 H E O I < L J Z > 9 9 L O = > D F 3 K D M A I A ? E @ K M ? H B S B K ? W J E > A @ Q Q 7 < O S J J N B O T @ M I J < O P N ] I Z @ V B L 8 E 8 E B P G A F M M N ; < < @ Q N U W W R T I D D P L Y Y B C 7 b A O 7 V 3 J 7 H ? 6 J F 8 P 2 ? < 1 7 < 5 : H = 7 A 3 . , @ . 4 B 8 ; 9 L E H A H E L E C = ; T < 0 N = D E F K 7 3 ? > A G J N I Z J F 6 E ] K H A : P . 7 A C P P 5 A A I 9 C 0 0 O C 7 < O B A A B Q 7 J 9 C = I I C C F E : . B U < @ ? ; 8 * D O 8 E 1 4 / 5 : ; S / 7 = % ; 6 S A ? P 6 O V D 7 ? 8 > < . @ 8 1 ; @ > = 8 J L D ; U > 2 ; A 9 ; 7 A 4 C @ / @ = 5 A K B @ > G R O 9 : B C A = I V P U @ B Q P F M 7 M C @ 6 ( 4 > 7 O 9 ( 5 - ) 4 1 * . @ > 0 6 8 . @ 6 8 / 6 < A G > 6 . K = 6 I 9 > 5 G 7 1 B 7 H , 8 ; A F 2 E ; ; 8 ? 2 U G P d = Z O B X R S U D b E T K V O K 3 D $ 8 8 A 5 G 5 6 - 4 7 ? ? > A 5 F = > 9 : I 6 N 9 3 : 4 0 - 2 + 4 7 J 5 3 3 1 : F & 1 @ C 9 - B 0 U @ = K 4 > > : / ; D 4 A 1 C C @ 7 9 : 9 E + : + 7 9 ? G 6 . 5 : : ' U F ? = 4 8 5 2 K 3 G @ 2 ; = ' / B < ; : A < F L E @ : 7 7 - > M > L Q N D = W N T H @ I Q N ? 5 * E D B H C J ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 30931debacc740598488d622e4be8010Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-27_00-59_0589 3130Seq 12310 12310_Costion_K1364_matK_F jmadera 96-Well K1364_H12KB_3730_POP7_BDTv3.mobSeqPop7zl&l}zuX>.,?br1^ $ne: sYPZt}sow< .fy]NUuP;[L]}&E\sVX;
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KB 1.4.1.82018-04-27 02:31:30 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 'r BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #a F DATA
#a F + DATA #a F rP DATA #a F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ % LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM P2BA # P2RL PBAS
PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ;, RUNT RUNT ; RUNT & Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC SPAC M SVER 4.0 SVER 'g SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH
phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 'r BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #a F DATA
#a F + DATA #a F rP DATA #a F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 5 FVoc 5 FWO_ GATC Feat . . 5 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ % LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM P2BA # P2RL PBAS
PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ;, RUNT RUNT ; RUNT & Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC SPAC M SVER 4.0 SVER 'g SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH
phDY big phQL c phTR phTR 6}k1$AZ}E]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGUNMCp:R2OMMoAQ0[A.#jTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"HHhQcig=RSwsKFMrRU)>`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9} P,hz9_"#kjFO-U[ds=:I1On(="]dr>y>V#|UOY] IKe4);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ils1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52rRU)>`V$];9'k0K3AQZ`K'H:{GKipM`{VL7f4 AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT b b C B1Pt B1Pt BCTS 'r BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #a F DATA
#a F + DATA #a F rP DATA #a F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 5 FVoc 5 FWO_ GATC Feat . . 5 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ % LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM P2BA # P2RL PBAS
PBAS PCON PCON PDMF PDMF PLOC PLOC PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ;, RUNT RUNT ; RUNT & Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC SPAC M SVER 4.0 SVER 'g SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH
phDY big phQL c phTR phTR