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Y (,,,III^^^{{{&&/99DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR>AU[]^^]\\ZYXWURQNMJHECA=;743/,*(&" ___"UUtyy)))565@@@KKKVVVaadjjstt}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 91451225ecf74338a4d01e56641e19b6Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_23-29_05883130Seq1230912309_Costion_K1363_matK_Rjmadera96-Well K1363_G6_RKB_3730_POP7_BDTv3.mobSeqPop7z&<Sgy*B]w):EIJIILORTSQOONLG?5-+2ATdmkaTKMXl|jXH8'[3 w]@!zpe\UQNMMORX_hr~+W.Kjk; _9gUJB=:641/,+))+/7ALV`is0TqmG wcSH@93,'$%)/5;>?>==?BEGFC=5.(#!!"&+19CPd[9}#%hgcC/$ #(/8BMWalvTMotV'_dN?4/,+,/5@Rm1BMTSJ6kUC6+$ #'+,+($!!"" 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"-(Qe& 2#"-lM;Bp%+A+>--6F$b.%5sXO>J9B4q8v8Ji]t?i<'ATAATAAAAAAAAACTGAAGTATAAAGACATACTCTGTTATTAAGAGTTATAAGTCCGCACCCGCCCCCCCATCAACCTATTACAACCGGACTCTGAGAATCCCGACCACCCCTCCTTCCTAAAAGCCGGTCCTACCGAATTCCGTAATTCTCCCTTAACCAATACTCCCATAGAGGGTGAGATTGGAAGGGTGCCTCCATCCCCCTTCGATCCAGGTCTAATTGGGAAGGAACCTCCGCGTCCCGTCTTCCCTTCTTCCGAGGAGATTCCCCCTTTCCTTTTGCCCCTTTCCCCTAACCGAAAGAGAGGTCAGGGGAAGTGGGTATAATCTTTCCTTCCAGGAGAGAACCCTGGTCGGGAATTTACTTTTCCTAAAATATGAGACTAAAACTTTCCCTTGTTATTAGGAGAATTCCCTCACATTGGGGGAGCCTCTAACCTTCCTGGTGTGGTACAGGAACGAAGTAGACATCATCGAATCAATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-27 00:59:41 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPttASPttB1PtsB1PtsBCTShOBufT!B$CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA +WzDATA +Wz< DATA +WzDATA +WzDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~fLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISBP1AMUP1RLQP1WDY|P2AMaP2BAdP2RL]HPBASDPBASBPCONOPCONNPDMFqPDMFBPLOCJLPLOCFPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT RUNT :;RUNT 36RUNT :!Rate RunN""S/N%BySCAN3SMED SMLtSMPL fSPACAYSPACBSPACAYSVER4.0SVER hDSVER,,fScal@Scan3TUBEG6Tmpr<UserphARphCHBphDYbigphQLcphTRphTRぅThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPttASPttB1PtsB1PtsBCTShOBufT!B$CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA +WzDATA +Wz< DATA +WzDATA +WzDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabvFVocvFWO_GATCFeat//vjGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~fLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISBP1AMUP1RLQP1WDY|P2AMaP2BAdP2RL]HPBASDPBASBPCONOPCONNPDMFqPDMFBPLOCJLPLOCFPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT RUNT :;RUNT 36RUNT :!Rate RunN""S/N%BySCAN3SMED SMLtSMPL fSPACAYSPACBSPACAYSVER4.0SVER hDSVER,,fScal@Scan3TUBEG6Tmpr<UserphARphCHBphDYbigphQLcphTRphTR<8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0k2y|L3M$z gMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhA1!oxBE*3-WVq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~6>udrV0HJA@vd*W]U_I[Ddg/