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F / 1 0 N ? 4 : 8 9 , , * G + 2 . & > : 2 1 4 < , 3 5 5 * / 5 8 7 " - < + , ( 5 4 6 0 3 0 $ , % - - - 4 1 0 % ( 6 ( 0 $ ) # $ $ , ( A < C 2 : @ D S z ^ b t n x q } k z r h w x s K ] T D V 6 < 6 > 7 4 , 1 : ? 7 0 6 : 2 3 - , ! , 2 ) 1 $ " + $ ) 0 , - ( + 5 6 + % < 8 6 F @ 8 ? C M Y U V M R G Y Y @ > L I @ E 9 6 5 C 7 ( 9 ' - 9 * 9 ) 2 4 . ; < A * 2 - F G B B - 1 S O U ^ h s n u i [ [ h O Y R F G E ? F C E D > C K V W [ T V [ v t y i U C R U D d V V ] ^ s s z s ] c D = L ; 8 / = 5 ; 5 ) > ; L R [ ` m b e f y s p b Z U R E P Q X Z K V U q _ x t k g U Y ^ H O E B G ? 5 : B 7 D > H K ; F A V 8 N ^ V c k { z j l P < S N K E J P M : > @ 6 < M ? 3 9 2 7 0 9 6 $ : # ' " % 5 7 ' ( * 0 / 3 5 A ' % % 3 ' 0 " & ' ( * = " ( ) % 3 ( ' % ) % " ' ) ) ( $ # ) " # " & # ( 8 1 + * / % + ( * , ) 8 7 & ) 0 4 4 P T A V Z N Z X [ W T Z . ; ? : 7 B 4 4 ? , + . C 7 % / ! 5 6 3 ' # 9 . 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' ( 4 % @ * / 7 9 8 ] C F Q ] h v ~ ~ { } s ~ | r s ` r d a k Y [ H E C c G P H X ] Y ^ ] ` p e _ w d z { { } c m b U l e K T R A V ] Q e \ Y M g p g d v u e \ P L H N 2 1 = > / 1 = = 0 0 ; 0 & ( ' , & & * # # " * . 7 ' : 6 = C U ] W I _ h h c p i X e X \ : K M K 3 < 0 , - , 9 2 + % - ) . - $ . 5 + - ( ) * < 8 J ; 7 = Y c g f p c | h m ` o l l _ ^ ] V i h ^ _ e f k u n | s { ~ u y v p e ~ i s v b e @ G T F 9 P V Z T P G f Z \ M l ^ h i m u i f s m j n p l w v b h l I _ B O H : 0 D 2 . 5 , 1 6 0 2 K I Y S R L I 5 : : E , 2 + 2 # # * ! 1 % $ ' 7 . 6 0 O N O H M N I f \ Q E H Z 4 5 : @ - 9 A ; ; * : : + N 6 3 9 ' & , % / ' ( . 7 4 , & + # " ) ' 9 9 > < 7 < 0 + * A 6 ? > ? G B E C 5 9 : ? 4 1 C ] @ 5 H T L \ H = < * 6 ( ) 1 ( 1 ' # 4 ( 2 . 2 + $ 9 3 1 6 @ ; : & " 0 & 5 * 6 . Q J I < A Z P c | a s p o u h \ i X g V L H X T > @ 9 G H 3 8 : 6 G @ C R ` _ f t j u ~ { u x ^ d Z P \ ` G P S S N N A I J H A T U K e ` k s u k k b k i W N U U K O C D K J J H < > ^ B U X p e g j ~ v v | s g N _ W ] p j V i P H Y j Z Z X q g { l o m ^ k | u o i q e U b _ a ` b J O [ = L = ; B : / E 8 M < < P D ? B = M Q C e Z R ] [ n k j c d b G e ? F K = ? 5 ; ' @ 0 @ - - / @ 1 7 ; Q F G C F Y V g a S a c [ ] Q m E A : + 1 , . - ) + * - / ! $ - 9 8 1 = - ) 6 ( . 7 4 + - . ' % - # - " , . ! ) 5 , 3 / ( 4 @ > > ; E T @ B K T G Q N c ` t m u h e d a R M ] b O X e m d q j q h f l g w v h t c t l s \ h K N D T T J F A > 5 ; E G K Q c ] W i \ S p u o | y r s q t z ~ b s m e M ] G ] \ F K L U W U e a ~ } v w z s k z _ Y Y ] d i S h m [ w o _ h s l s r j d v v b f m j d i O ] Y Q R E W M J [ T d ` l e [ W q n m h n v b l ` R V O G L N P > ; N 8 ; , ) $ ) 0 * 5 + . $ $ . 3 2 2 5 & ' ( + % % ) 5 # ! " , > * : ' % ) . + 4 , # ) 7 , * : = % 3 ' 4 0 0 2 C = 9 F ; ( 9 6 < , < : 7 5 # % & ( ! 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( ,,,III^^^{{{ &&/99DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR >AU[]^^]\\ZYXWURQNMJHECA=;743/,*(&" _ _ _ " U U t y y )))565@@@KKKVVVaadjjstt}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: cba003da544a496b80e1828d5153813eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_23-29_0588 3130Seq 12309 12309_Costion_K1363_matK_R jmadera 96-Well
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e frGGGAAAAGAATAAAAAATTTGTGAGGGGGGCGAAGTACACGGGAGACACGTTAGTAGTCAGAGCCAACCCACGTGGAGAGGCTTAATCCCGCCATCCTGAAACCGTAGGCTTCCCATATGAATGGTCCCTGGGCGATCCCAATTTTGCATTCGCTCCGGTTCCCCTGCAGGGGTGTATTTGAACAATGGCACCGAGCACCCCCCAACACATTTTAATAAGTTATGAACCTCCCTGGCCTGTACCCCGGTCAAGTTAAAGGACCCTCGGGCACCCTGGCGATATATCACCCTGGTCGAAGGTGGAGATATGGAACGGGGTGTATTTATACCCCACCGGTGGGAACACCTACTTGTATTGGAATTTGCATTAAGTTGCCGACGATACATCCCTATCCTTCTCCCCTGTCCAGTGTTCCCTTTAATCGCGTCACAAAATTCCCTGGATCTCTCCCTAATGGAGTAATGGGACCCTGCCGCACTCCGTTGTCGCCCCGTACCCCTTTGACACATTTATCGCGGTATCGCTTCTCCCTATAGAGGTATGATCGTTCCAGCCGGTGGGTCCCACCGATCTACGGAAAAATTGACATATTAGGGTATACACCCGCACGTTGTTATGCCCTTCAAATTTCATTTCGCTTTATTAGTGCCACATGCGTAATCTCTGCTTAATCCGAAAGGCAGTATGGAATAAACCAAGAACGTAGCCGCAACTGCTCGTTCATCAGTCCTCCGAGGTTACGGAAGGGCCGATATGAGTACAAGACATGTCTTACTCCAAGGCCAGCACTGGTTAGACCGCCTATTCCACGGGGGGGGAGGAGAGGGGAGGGTTGTCCCACACAATACCGAAAAGCGGTTAAACGTCTGGTGAGCCAAGATTTCGAGTTGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-27 01:00:15 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt o ASPt o B1Pt n B1Pt n BCTS } BufT ! B $ CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA * UF DATA
* UF 9 DATA * UF DATA * UF c DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ |+ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS 9 P1AM } \ P1RL } U P1WD } c P2AM } q P2BA } } x P2RL } j PBAS } } =a PBAS } } 9 PCON } } RO PCON } } N PDMF q PDMF 9 PLOC } G PLOC } @ PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
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RUNT RUNT :; RUNT 36 RUNT :! Rate RunN " " S/N% 9 SCAN 3 SMED
SMLt SMPL f SPAC AZ& SPAC 9 SPAC AZ& SVER 4.0 SVER } SVER , , f Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR 3I This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt o ASPt o B1Pt n B1Pt n BCTS } BufT ! B $ CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA * UF DATA
* UF 9 DATA * UF DATA * UF c DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat 0 0 GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ |+ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS 9 P1AM } \ P1RL } U P1WD } c P2AM } q P2BA } } x P2RL } j PBAS } } =a PBAS } } 9 PCON } } RO PCON } } N PDMF q PDMF 9 PLOC } G PLOC } @ PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT :; RUNT 36 RUNT :! Rate RunN " " S/N% 9 SCAN 3 SMED
SMLt SMPL f SPAC AZ& SPAC 9 SPAC AZ& SVER 4.0 SVER } SVER , , f Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH 9 phDY big phQL c phTR phTR }g#XrV?bTio0<57#u?GG;Xj`:WnJuYSjMr[Vu`?+rh0v4"qp~!U#Mm[*&`cz"B``,#th2k0[+D:rF(5,fc-,RIlVinJilZbCd]kGZN"RgxwJ|*I*]E64Wg?UWU;j(6oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? S8UXabCDV_mi/fcGHO5QfOl>o %> Ax7cXF$hHz))OH"vrn;:QV/|!)3,\K>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Ou