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___"UUtyy)))565@@@KKKVVVaadjjstt}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 783fd33718884cae9c25ed02036e0922Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_23-29_05883130Seq1230912309_Costion_K1363_matK_Rjmadera96-Well K1363_E11_RKB_3730_POP7_BDTv3.mobSeqPop7z(*+.1478:<@EKPTWY]ckt~sg^XQKD=60,(%#!!#%(+,,,++-.000.--./0000/.--,+++*(&#  "#$#!    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IP=Sh<DAu6%'wVJZRiA  7 #; $hhd );x%6Bgl!1 !TTATTCAATACTCCCCCAAAGAGAATATGCGGTATTATTAAGAGGGATAAATCCACATATACACCGGTATCGGGTTATTATCACGGGAATCGGCGGATCCGGGCGAGACCTCCTACCTAATAGGCGGTCCGCCGCGACACCTTAACTCGCCAGAGACGACGATACGAACAGAAGGGGGAGTAGGGTCCCTGGATCGAGAAGAGCTAGAGCGCCACGGAAGAGGGGCGGGAACTCGCTCAAGAGAATGCGCATCTCCGCAAGAACTCGTCGCACTCCAGGGGAACGACTCCCCGTATCGAAAGGGCGCGAAGAGGAGTAGAAGATTCAGATTCCCGTCGCGCGAGGTGAGTCATCGGGAAGACACCTTCAGGTAAAGACGAAACGATAAATTTAAACACTCAAGAGGAGAAGAAAGTCAGCAGGATTCAGTAATTAAACTTCATTATATATCACATGAAACAAGGGAATATTCAAGAGTGATCATAATAACATCAGCTTAACAAGAGGGGACACAGCAACGGTATCCTATAAACATATATATTCAGAGAGTTGAACGGGGAACACTTCAGGATATGATTATCATTTAAGAGGATGGTTGATACAGAGGGGAAGGATATATACTCATACTCACATATAGAGTAATATATAAGTATGGGGAGTATCTCATAACACCCCGAGGGGGTATAGGTAGATAATAGGTGTTATTATGACAGTGACACTAATAGTACACACGAGGAGGGAGCAGCACATGTGTGCGTAGAGGCACCTCACTCATGCTAGCACGGGAATGAGTGCAACTATAACTACATAAACGCAATAGAATATAATAATATATGCGGCGGTTTTAAATGTATGTAAGCTCAACTTCTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-27 01:00:30 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSu`BufT!B$CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA )SDATA )S80DATA )SDATA )SlDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~sLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS3P1AMfUAP1RLfNuP1WDf\ P2AMfiP2BAffpqP2RLfbPBASff6PBASff3EPCONffKPCONffGPDMFrPDMF3)PLOCf@PLOCf:PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT RUNT :;RUNT 36RUNT :!Rate RunN""S/N%3 SCAN3SMED SMLtSMPL fSPACA]SPAC3"SPACA]SVER4.0SVER uUSVER,,fScal@Scan3TUBEE11Tmpr<UserphARphCH3@phDYbigphQLcphTRphTR eThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSu`BufT!B$CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA )SDATA )S80DATA )SDATA )SlDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//{GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~sLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS3P1AMfUAP1RLfNuP1WDf\ P2AMfiP2BAffpqP2RLfbPBASff6PBASff3EPCONffKPCONffGPDMFrPDMF3)PLOCf@PLOCf:PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND RUND RUND RUND RUNT RUNT :;RUNT 36RUNT :!Rate RunN""S/N%3 SCAN3SMED SMLtSMPL fSPACA]SPAC3"SPACA]SVER4.0SVER uUSVER,,fScal@Scan3TUBEE11Tmpr<UserphARphCH3@phDYbigphQLcphTRphTRa,pEnO"|#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? 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