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L [ O B \ H K X T . H P I R L R C ? R H 7 P J N A R P K H O P Q M X Z W V U J ] O X N I U B J Y L Q ? I N M I P ; W 7 S H : Q O K Q R @ T O a G K S M K ? U ? M B P Y C S K = K ? E C D H D G < @ D P I U \ J H K S Q K I L S A H > E @ R F ; ? ? 3 F : G E D A ? : H [ > L J L K U P G J D I T 7 W H 2 ? B H D A I O D S 9 6 = B I 9 @ G ; L @ T K K U M Q K @ E Q R W O M V E P c X \ Z a ^ c h \ f T _ M P L R G @ T M U O M ? G P F H K X U K D D @ I F T E R P R P B H F N D M I U I H e J H M @ 7 S X W A Z > O I A N S b N F R Y L U [ N L T J X Q F J L O C G O ? G N N d ] V W P T H H V V V _ I Y O _ L N b Y c J ] Y Q h _ _ R W S K D G S G K U k g W Q h p S \ X G N Q Y Z N T \ Q @ M [ P N I Q U \ T X K R C L J E X Q T X P Q H T R V G = ; N : I N K K K A X J B W V Z P L E H L X R C O A H U M I F L @ ] M C T A D S Q D X R O T S ] Y P L S R T Z [ T D L Z W H C U A @ T G Z O P S ^ T c U m T N X W ^ L b J R S R R f \ M I X F D E N > A Z F C M Y D O O Q = G L W \ O W c Z Q [ [ U N J V T C X K T [ H U K L P Q P \ h W W V [ _ ] \ o W T T [ U V S R c [ b W p f d Y q k T [ \ X p j b Y W e [ ] L d a ] ] N [ S Z [ J X c _ T _ N R _ ` N [ d U > P ] a R T U U E Z L Y K F T G ; I C K B < B @ ; D D 0 E J G @ ? Q T P M ? S V _ _ [ A \ G B Y Y d _ M D C K Q f ` ^ Y [ V R ` W K S T K G G N _ N L > G Z S H V U ] ^ X A N M S H R R N X Y X c ] W V b P N ^ ^ J M _ a i ] b a i [ M _ I K Q T R K V P ^ h N S T _ S P L _ ] V N S L O P V M P L D G S R Q I O @ N F U Q ? J O Z H R ^ R W V E N N C [ H R U S L G D L L J R S I S V W Z L J ^ e P ] N W F N I P J P L U W D F Y g a F V J _ R \ T [ [ C [ N Z [ Q E W ] L K X B V U d ^ W U n Q P g L N [ I \ h X K J T V P R S R W Z U Y J c d U P Q C H E I P K ] Z ] T V [ O N I _ E L U V > H H U A < 2 ? L 6 Q R L M E A X G I P M H O E U K [ ; D T A R J M [ Q C L U / L I A F R O F W Z _ N M B H M 9 P E M L M C M V ^ T T T Q _ K P c 9 R Y L ^ Z f Y \ c \ _ \ c ^ S S d W \ \ ` g \ g j n W X X e O [ O > I A M K G @ 9 L L : F : Y J R J E C V D K G O I C A O 9 K ^ ? J I H F = Q W S ` J N L E V _ X F [ M _ W Q e ` Q G L C O = C T N M / A U ; J ? P \ N = E C M O 2 P U O Q T ] Z b f J L ] O R P N = K J ` [ d m p \ R S _ T O M C ? Y L S F G E O M S \ K K M V W O S J M O = > S E D R ? K H 5 P K W I D L ? 9 S = D D Z H T S W ] P E T I P G M C C M H 7 N @ G V L ? M 1 H A Y J F J P L K M 6 I P ; O M K M M O I J Q I E K I < S M O c D K I Q H H A I M 9 V J A ; S > 3 M T A G W M E 8 Q I J Z < K C R A M G H J - Q B B N H 8 D ? C 1 L 9 C > V O J O < = < @ C 5 D M @ E 1 8 < N N K P O K H R T C J I N J O N @ X F 3 P B S M N \ @ ^ Q D H C I Q T B K D J F 8 < < C ) 1 G B < Q : > E 5 F > H 8 F K N B 8 8 = E 6 K ? F C B = N F C I = M E ? S O G < H C N 6 E A < \ < C N G F Y L T ? U > C O S C O @ F 8 / : O J : = ? L @ B 8 R < ? C A J @ A D E H O 7 M P S J P N R E M M K ) M U < ; I 4 S 9 K B 8 D O _ P K X Q ] Z d U T Y g W E F D J > C M K K D M H F H < A @ = D / ; T G ? ? J ? < 8 - - 9 ; 7 : ? A D G Q E 6 L < @ @ 9 S 9 B C \ 6 I X @ R P P Q S N = P N D O ? E A H > ? B C Q 9 D R I > G Q T M F N V = P I F J H @ V Q A K ? Z Y I K M X E @ @ J < C I F 6 J _ N Q K M S G N I O P O L ; L M D @ T M 3 H M A L A ? C G V B A < P E Q E 8 N > H R N D D V D G V I C K B G G , F E < 8 6 4 2 6 E 8 P B G I C G A , F 7 E V E L B L H a U ? F K @ H 4 R = @ A 7 P C 9 H G O ? : 9 J < @ P 6 : N P ? G H C > = 0 I N B 8 E N ; W L W K J : H W > : W Q J U Q > K K F ? 2 D J W M G L F Q T 5 G Z O F Q M Z U Q M _ L D J > 8 G D D S M E K K A ; F > M B N < > J N F J K M J D M R D F B B Q N E E J ` G U Q P O O K O S E G Q K S C H D G G G , G @ C ? E K R M S M A 5 F E K I I L 5 J K 6 ; @ M I : = ; T F L H I > N G 4 S @ > O G 9 G 8 _ E Y U L T Z B I @ M M : F 9 > 1 : Q & 5 ; 8 6 O ) 6 7 @ I 7 9 6 0 < B B A E 8 7 2 K = C > > > D 8 @ L D O 2 8 : . G < ; S 8 K 7 D L K @ L K Z B J Y 0 E C ? 6 U A = C / : ; : 5 : E / L + E J ; 9 I > D H 6 < = E A K 9 : 7 5 A : , L < = 9 7 A 5 C A D N 5 8 A 1 A : = 6 > 1 = M P A @ L J 3 + @ A 0 , I B B = J < ; 2 ? B B 9 O C J = E = 7 K A ? + J 3 B D : @ ? ; < 9 5 1 B 9 B 8 < > ; , < ) 2 . B 0 : : 3 4 ? 2 9 = @ 8 5 : 2 1 . ; = A J D C 1 6 ; 3 ; 1 9 A E . ? ? 6 + > ; B 6 . G ; 0 3 0 I 8 7 3 O D : @ : 9 5 ; ? @ = 2 = F A - B M 5 / B 6 * 1 ; C E J > / 5 > 3 5 9 = ; 5 ( 6 ? 5 = G 6 6 0 ? 2 ; F J = > : E E : 5 5 @ 0 E ; < M C 5 K 3 ? D B @ D < K A N B E B I B = ; 5 * 2 9 ' % M 7 / 5 B 9 4 K 5 ? 9 7 = . @ 9 % * A + & 5 8 / > / 6 0 ; < - 1 = 9 9 H = / M 5 : > 4 6 0 ; D D 7 4 * 9 0 1 D ; 1 = 0 G 8 9 F 8 7 U @ 3 > 4 6 = C 2 2 ; 5 5 E 4 : 9 6 D A : 8 6 : 3 L 1 = 4 ; 8 H 7 < 6 / 9 < A 5 . 9 : A 2 4 @ 9 : < 4 @ 8 > < B : : > ; G E B E ; B B : - 5 A 4 7 > - 7 ? 8 6 1 < < 6 C > : 8 2 9 0 - A ? 5 N ? E = 6 > 4 6 ( 8 4 5 < 7 ; ; 4 = = A 9 = I I : 1 < 6 = A 7 4 7 B A B A F C . = A C 7 8 5 9 9 D A 3 G K ? > C ? 7 H E 7 > @ H ; A @ 8 C @ 9 E / H 1 ; = ; 2 9 ? L G C I D G = = F H P B L I C D E I J H E D G > I D 7 H D 6 G L E B < I < H 5 M B 0 ? = 8 1 : V = 4 Q K O N E B C 1 : O 6 D 6 5 I ? . / 2 2 1 . 1 3 = 7 J ? 0 ; @ ( 8 = E - 0 6 + 3 - C 6 - C B 5 > D < : 3 3 8 5 - F / Q 4 , 2 D & D 2 E D < H U > M ? R H : Q I K @ a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( ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR oKVZ\\\[[ZXVUSRQOMIHEC@=;8530.+)%# _ _ _ U U U y y y '))555@@@KKKVVV```jjjtss}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 83681f40480443f39b184f07df1e4142Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_21-57_0587 3130Seq 12308 12308_Costion_K1363_matK_F jmadera 96-Well K1363_F10KB_3730_POP7_BDTv3.mobSeqPop7zU ..NC P!=Wirv{;(WuuN|os|ze@ 9GqpSyF$ 4WurO#8]K\9"13f
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KB 1.4.1.82018-04-26 23:29:30 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #h F DATA
#h F + DATA #h F rl DATA #h F < DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM A P1RL A
O P1WD A S P2AM A W P2BA A A P2RL A PBAS A A PBAS A A G PCON A A PCON A A PDMF PDMF + PLOC A K PLOC A PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT 9; RUNT RUNT RUNT 6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC $ SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH B phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #h F DATA
#h F + DATA #h F rl DATA #h F < DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ' FVoc ' FWO_ GATC Feat . . ' GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM A P1RL A
O P1WD A S P2AM A W P2BA A A P2RL A PBAS A A PBAS A A G PCON A A PCON A A PDMF PDMF + PLOC A K PLOC A PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9; RUNT RUNT RUNT 6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC $ SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH B phDY big phQL c phTR phTR Py]]~I8e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|L:hmt$lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,Hb&^]`Q;fu:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NBrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa$Fl@i2<`2fvn[?a9TghW~^N@.]neZ_kjh'SQ AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT b b 6 B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #h F DATA
#h F + DATA #h F rl DATA #h F < DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ' FVoc ' FWO_ GATC Feat . . ' GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM A P1RL A
O P1WD A S P2AM A W P2BA A A P2RL A PBAS A A PBAS A A G PCON A A PCON A A PDMF PDMF + PLOC A K PLOC A PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9; RUNT RUNT RUNT 6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
SPAC M SPAC $ SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH B phDY big phQL c phTR phTR