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"'*!""    , /6''//)'         *&              %&%$ "%(&)$3)#                                       '*        + #                                         3            & #                         *"                        $ # "  "   #(                       ,+ %                        2Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]hkmnlkkiigffca`][XVSQNKIEDA=;8530.``7VVV{{{   ---999EDDPPO[ZZfeeoooyyy                                                                     <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a403367e5d7d4947b935c6deab6406e4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_09-41_05813130Seq1230112301_Costion_K1362_matK_Rjmadera96-Well K1362_H6_RKB_3730_POP7_BDTv3.mobSeqPop7z:?EKOQOPYn0=BCCHReF{d`* *<9/6_yVc "y$%')l+ ,-.4../0`1w23z4 4P4i4}44443520//.l-,+)'%2" OiB S| ` ? 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"                                                  "                                         !                          "                                                  "                                      FZB O1 *LP=S/?+W.T F7\  <'CR@]3Q.S([Y a Q31L UK^HEA%I:I4K3cY F6c,;$ B*)Y-I> a.XDU>BDJ: X6IOIEH\$)1/<3*' W Bc;B;#^:DZ,LWM/4HJG8G]J5$8(=]R!Y"#.4IR=.EFBa)>/?194AWX:K:F^^@ ZQ*Fb0 F*O%QX]A ! JK\V7(Q-HQ_9IH.A9687<JZcU:'(K2TC8WW 9CB$XD^;Z*/#Q7VIP)aCK*'HF%_. H4]_TG!I; U1Cd^# QL3DB2&AQ=XU!8@WbB[D.1; \I;;YS@S2V bH23#+P"a.];8`: #L=N&6QFDNV:7 .6/C0P*M]=JW-Q F@W;)=._bN:YP* .DOTCS@5bX5 2%-FD]Q!84J48UYCN1}H&qu|Oi]@K&'Y y AM^a~2p;N'drlf.>Boi: w^{0 6 /RFj@6 s:pK)PTs8 Y itt}$mu;?+s{ EL}-,W9YR~QO;M[.l p\+7bg|1L=SG}w"C:+^<E#It&=Hm5v9iVDrY+)L)ReTn4&Bd5PrG+/LT[vT~~F: 1P[tt"(`UV~ )OR;=831(a\A;i{R\9]`($F8(VS Srf~ ky7:|x-0V'M3oI6$ jH#k-E]8)RMfzx^x@^KX%4]7L@*=(gQ\2<b9IfK81JRa00( ] -%6L!S%1[V4 :I /) %?'"."B;& *$MiB' #;'4$- DN S 1n5BL9ZO8 N*0V_0! *AlI2)   G.M ./|! l,A5O,X=/H2-%:AhNDY~?D \}\CK*& SG PZO`1IVzXc@+V ,  ~ W73 f+X0)dPpp@pP,pZR~sVc"Y,p KLP|-^Pf^smEJARxix}e-!z=L.H,B*T 8RdLd6_~` u4rJ dTbA]1>+rvJ6=gM?1t(p3Wv?v +6\FEb0uc@>G%p,aQhm{RY*pVP@{21yDgB&wUdHlH ]WqpOC2~ F9! :"F9t/EM>}F:H?Q:2-CKCyyjXLN;VC>m3a ` =C<1^}GwUL16ZtBxk6 ;&y&sdQtFJn* Cc4ggJ;$E:D7+/0rX+o"b+n1 '7O l>(cA&7^iRgoCxls y@H6QsX\,"d1R $q<" }:?%;pb"av>_vkefO06G4>ipwaYY aI J#>IWcq .I!euQ'.4JJZ' 'o v.*"<1(BCYLElS$K< 6?BEw%gz.dg D\2f #2a,-:#K8ZHNGGGAGGAAAAAAATAATTGGCCCGGGCTCAGAAAATAAACACATCCGCCCCTCATCCGTACGGCGTACCCGAAATTAGGTGAAAAATTCTTCCGAAGAACGGTTTCTCGAAGGGGCCGTACGAGACGCCGGTTACGTTCCTGCGACTCGATAATACGGAAAAAGTCATTAACGAGCCTGCCCAACTCCTTTAAGAGCGTTACCCTTTAGGGAGAAGACCTCTTATTCTCGGACCGTAAAAAGTAAAGCCTCACCTACCATCTATGACCCGAAACCCAACGGGGAAAAAACGGGTAAAGTGCTTTCTAACAGATTCCGTGCGTGCTGACACCAGCAACATATCTTTTGGAAAACCACTCCAAGAATAATTCTTCTCCCCCCCCGTGAGAACGCCCCGCGCCAGCAGCCTAGGCTTTCGTCCCTCAATTCCAAATTTAAGTGCTGTTGGTTTGCCGGCTTATGTATGCCCCCATTGGAACAATGAATTTGATGCCGAAGGTGGTGGTCCCTTAAAGTTTCCAAAGCGCACACGCACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-26 11:12:35 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSiBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA +tVDATA +tV;DATA +tVDATA +tVDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~hpLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS@uP1AM0UP1RL0QhP1WD0YP2AM0b(P2BAfXP2RL0]PBASBPBAS@PCONOPPCONM8PDMFPDMF@PLOC0IPLOC0DPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  )!RUNT  6RUNT  4RUNT  Rate RunN""S/N%@mSCAN3SMED SMLtSMPL SPACAX$SPAC@SPACAX$SVER4.0SVER iSVER,,Scal@Scan3TUBEH6Tmpr<UserphARphCH@phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSiBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA +tVDATA +tV;DATA +tVDATA +tVDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabxSFVocxBFWO_GATCFeat..xGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~hpLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS@uP1AM0UP1RL0QhP1WD0YP2AM0b(P2BAfXP2RL0]PBASBPBAS@PCONOPPCONM8PDMFPDMF@PLOC0IPLOC0DPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  )!RUNT  6RUNT  4RUNT  Rate RunN""S/N%@mSCAN3SMED SMLtSMPL SPACAX$SPAC@SPACAX$SVER4.0SVER iSVER,,Scal@Scan3TUBEH6Tmpr<UserphARphCH@phDYbigphQLcphTRphTRR)U`l5j6!X`7.qT*uq&6i}8Amabj1A6rd)ziA=sUOzj{>ZHm? 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