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"                  &!     #(    %          #         %2.+'""                                   2Y,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]hkmnlkkiigffca`][XVSQNKIEDA=;8530.``7VVV{{{   ---999EDDPPO[ZZfeeoooyyy                                                                     <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7fcb391f4e9b46a1a0ca3f6f37cc190bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_09-41_05813130Seq1230112301_Costion_K1362_matK_Rjmadera96-Well K1362_E2_RKB_3730_POP7_BDTv3.mobSeqPop7z#)-.-++-27;=<;:<?CDA:0'#&1@Q_hkid[RICBFNYdlnkd\VRRW`n~ #CjCt\0c?_  J  _ paS71ERXdd  !H!"#_$*$%%&=&^&i&h&b&R&.%%m$#"! r7%N Tk' q & s U = G x/pX3}3@C4L95 p R ^ & k:q P4{$f  O v q @:~=$q2mYS[p>a!Fg~xV#?)ji"cI8.**+.149=?@>;6336?L_w  wW9~yskc^^es%NuhG#}gVI@:777:<>@BCFJOSWXYY\`hr}xqlhd\RG=50+'" "#       $*./,'!   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bcpKB_3730_POP7_BDTv3.mobtermCGGGTTCTCCCGCGCTCGTCGTAGTACTATTGGTGGATTTTTGCCTGCTTCCCCCCGTGGACATATATAAAATTCAGGACCCCCCACTAGGCCTTGAGGGGGGGACCCTACTGCGCTAACAATGTCGTTTTTACACAGATCCCCAGGTGAGGAGGGTGTAGGATACGGTCTCCTATTTTGAATAGAATTCATATTAAAAGAGTTTTCTCTAGTTTTAGTCCCCATAACAGAGAGTTTCAACCCTCACTCAAGACACCTCCACCAAAGTGTAGGGACTGAGAGGGGATTTTATTTTACTTTCCCCGGCCTGGTTGCGAACACAGAAAATATGACATGGTCCTCGACTGACGTAATTTTTTCTTTCTCTAAAAAAAACGCGTCCATCCTATAGAAACTATATTTTTTTTTCCTTGCCTGCTATTATGCATGAGGTGATCCTCCTCCAGGGACGATAGGTCACCCGTCCATCATACGTCAAGTCCACCCTACATTTTGGCTAATTCTTGCCCTTCATACCGTCATCCCGAAAATAGAGGCCATAAGTAATATGGCACTGCTCCAGACCAGTGTGAGTAAACTAGAGACTATTCCATATCCCATTCCGATTGATTTGTATTGAAAAGAAAATAAGATATGTGTTTTGCTTTCGGATTCCCAACAGGACTTGGCTTCTCATATGTCCCGGGTTCTCCCGCGCTCGTCGTAGTACTATTGGTGGATTTTTGCCTGCTTCCCCCCGTGGACATATATAAAATTCAGGACCCCCCACTAGGCCTTGAGGGGGGGACCCTACTGCGCTAACAATGTCGTTTTTACACAGATCCCCAGGTGAGGAGGGTGTAGGATACGGTCTCCTATTTTGAATAGAATTCATATTAAAAGAGTTTTCTCTAGTTTTAGTCCCCATAACAGAGAGTTTCAACCCTCACTCAAGACACCTCCACCAAAGTGTAGGGACTGAGAGGGGATTTTATTTTACTTTCCCCGGCCTGGTTGCGAACACAGAAAATATGACATGGTCCTCGACTGACGTAATTTTTTCTTTCTCTAAAAAAAACGCGTCCATCCTATAGAAACTATATTTTTTTTTCCTTGCCTGCTATTATGCATGAGGTGATCCTCCTCCAGGGACGATAGGTCACCCGTCCATCATACGTCAAGTCCACCCTACATTTTGGCTAATTCTTGCCCTTCATACCGTCATCCCGAAAATAGAGGCCATAAGTAATATGGCACTGCTCCAGACCAGTGTGAGTAAACTAGAGACTATTCCATATCCCATTCCGATTGATTTGTATTGAAAAGAAAATAAGATATGTGTTTTGCTTTCGGATTCCCAACAGGACTTGGCTTCTCATATGTCC $q %8KVjy1IXf%2@Y`w#*:E]hr*8BN[gr '3=IUamt -7DO^ly+8FVbpy   ! 3 A K W b l {  # 6 H S \ j u    ) 4 C L W c p {  $ - : G Q a k x  * 7 A P \ g s |  %1=HU_lx ,8CQ`p}%3=IVbn|(7AOXdr})5CR^o|(2>IWdly  .<FS^iu +8@LYes %1?IWbq#-=HWeu *7?HS\lw+<GQ[hu '8ALV]gs0<DQ\fp|'6CO[fu.7ENZdv .8DO]em} &0=J\jv    * 6 F R d t !!!!'!2!IWdly  .<FS^iu +8@LYes %1?IWbq#-=HWeu *7?HS\lw+<GQ[hu '8ALV]gs0<DQ\fp|'6CO[fu.7ENZdv .8DO]em} &0=J\jv    * 6 F R d t !!!!'!2!08O=F cK >U9'J(@`SJ!Z187+NcKR@.ZZA/E6Q5( CNS 50L H,0?>WLUcI`O*%@`6*KIZB1*TJ4"Ua%VQ9Z QI6!V<@??- Y2P@6X;Q; X#6)0X7AcR]N1`U IVWH$2c\4V$5=47@.Y)Ga#)EFdM[IUS3,DW7>T/!]R;[5%R IB ?MP#\"G>41L>abUCEN)V^);`;W^,YU5>RU&E 5TI9\X3 \!#/="^<^+NY*R-Z']\Q\"XL7a3 Y#A/bDYTWQN3W:  *[H &+L#>2>>9>7.\X2A =S='#:UK)L S6@\^SL[0R:#?T9+` 2?#2(2G#K&&"(M^L/SD  K?:J6+SOEH2GG7F@G62@JWP\4[5`:R !?(HZ5LC$GZ GZ22Ea2^H!ACY !2Y2 \/5 !G!G"P'9DT5LC!!I=IN:]JW&6i.1$F' [j8L [`8WiT:/vyt lCk4nC2 :jQK0 yz( 0Fx"yN+`4C{m ygxe7!0!V~?SQ@i{WZk@vOk- ?uQM }*g ?n&|c:e5pf4y:NB,Q?4T^&S[i%:.B 0$!]5:0>4BszY@ogoh0{n2UCDP ;etE">u( C3^;{Wg2, - <XII^93[`</a{oZ3FsT]v~75w 0H {G]q*Pu y$|{B84Qv *@>)b.Ie1+ 7?pj6*P]k~ |42*~8z+M tRiE1H8 J^#6/ (%1[a X9Q9(78RSJT+9=7o wepXA+.t]~W7M+7Ic,cDq ;18}JHX0 i(xG J?@f(#L-,% )$&    " (u&D) 8PP'. .3--:G5H? 7/a: ) H?*l_%gO>Ei #=AC+. 6@dR"DpY &: >-I$=A U2'PyH 3EBc]ZHPAj#0P!5=Z4>4444=!A>>(444=zlx>03>/Ieee DDDDk3D33eeB (DoDDPDk33De[lk>YPIwIsIPIsPPPsPIEIApsPssPs>sJJsooJJesoo[sooo[osoo[ssss]ssssoss[sosog[oooo[JoooJ[[sooJJJssJs[[sJoo[[ooEoyoo[LJnL:5,8EJ^ GG>:dy;s->U1:Dvh Jvbe{p3#"[W, 5dm)6p8~1,W1Pu*{+4%N{[&;^1#9wS"(Up2A]upc1=! .w{P2+2g 'l<,q.qg:, U{ )w3'^'n[W:"J~c2TzM<X8WC:"aYq /"'( AK%gu~7GFf#?;04C[Z??zEyZUG#o^R%j,a^"d1J 7'EjH]GpdUe&8xppf"??;pI,iRqC w( ~xG8x"p,H+AIhfjJ*iUe 4` TMT`/F=W1vv"0kjZds]!$3{@H}>Zpo3aCb\Ut_|Vd -'h/*U"{x^I? a9eL#i]>ZRhP\Ro0pG<u,C ""enN*PwOulbD!HCn dzc?do$f*glB\c{;GffnDOOcsm2T^7!Jrs3?a$q=N;Z|`ql2.:f#iUr5=pfzNY2\9sJA+90<W$. H@{>DX39.hKTKYJOrt8i8/^y2"]NvjU]5 *)10>fz;SydBM8oT iq|#08> O2 ;EKJ^B-.Q4q|F)}WmV}&|{,kP4_T!aJ]>tgLlovACNAAAAAAAAAAAAAAAAAAAGAATAACCCTAAACCAACCGTTTCCAACATGGAAAGTATTCATAGACGCGACGGAATAATGTGCAGCCTGGCATCTAATAACCGGACCAAAGATACCAGGATCTTCCCCACACATCCGGTTACCCAAGAATATAATGGACGCGTTTGTAAAATACCCTGGCTTTTGAATAGAAGGTTTTAAACATGGGTCCGGATCATTTTCCCCAGATATCGGATTTATTCGTTAGGTAGACAACAACAACGGTACGCAAAGGATGTCTTTTTAACGTGAAATAGGCATGTCTGGGACAAAACCCAGAGACCTCAGCCTTTGCTAGAAAACAGCGATGCTAGACCCTCCCTCAATCGTCTGCGTATCTTCCGACCACTCCGAATGTAAGGACGCCGAACAATACACCAAGGAAGGTCTTGATAGGAGACTGGTCAGGCGCGAACGCATATAAAGGACCCAACTTTAAAGGTGAACTCGGGCTTTGTTCCGAACAAAGCGCATATTGACGGAAATGTGATCCTTTAGAGTAAGTACGTAACACCAATGCCTTAGCAAAATAAGGGAAGATCTTATCCTGCATCTTACGCTTCTGGCGAGGTATAATCTCGTTCTCATGAGATAAAAACAATTCCCTAATAAAAACGGGACCACGAAAAAAAATATGAAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-26 11:12:09 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTSpBufT'N,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N6DATA'NDATA'NDATA'N DATA *UDATA *U;WDATA *U DATA *UDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT šEVNT ¦EVNT²EVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~oFLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J0FkVM=j(\[ cV#JX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0t[XAn@Bg-~!iB-grw]2H\JKm?HKz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAa^{?nNL)H"i=Q<,{AmXXt;*hZyP4)@Dx`575^@!&TEZvKwKos5I|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#