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b \ ] i S d ^ [ \ h A I X Q F 2 B 4 = = 9 = D = I J K @ M W Z X M ^ E ` > A ; J = L + : D 5 0 @ 7 A / 6 ? ? G D : 9 ; F : V = ; S ? Q U F F T K I K 0 ; H , 9 ? Q 0 B ? H > : 0 " ? 3 / + ' / ) : - - & 2 5 $ 4 0 # 6 - 6 . / 1 + 2 * 0 / - 3 . & 0 : B / 5 < : 0 6 9 / 1 7 0 # # : 1 & - " # # * 2 ( ) * ! 4 6 ( 5 ! . 1 5 / / ; = 9 4 ? 9 @ A . 7 7 . < , : E : C > 5 ? = D D X [ Z G ] [ U L P @ E H M Q X O : F K V F S P ^ p Y W [ \ X T X 5 G F I ? F B H X V K K X Q O J K X Y K U a Y ^ Z Q S T Y X P K M Q M a j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` c b S X T L W M W W _ I H H Q W Y M ? I H A = 5 C D 7 8 8 < A < = @ ; 6 > ? A < / 6 4 ) 7 @ < , A 7 7 1 5 > , 2 = F 6 > T 6 : ; = 8 @ 2 9 ( 2 5 H ) < ( ) 4 + 2 5 2 6 ' . 5 ! , . , ' ( * $ 1 6 - : 7 @ E E A 0 > N 7 G P ] E U Y ^ G G E H = H L N L ^ X L E N @ I [ Y E C C A 8 > H A < T 9 F F C I P N J ? P O N @ O A H < H ; 6 = 4 + + ' ( - & $ 4 4 C 0 ' $ , " 8 * / 8 ( F " $ ( ) + & # ' 1 9 : 1 $ - 4 , + / 2 8 B 9 7 9 5 : F c ] [ L k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2 Y ,,,III^^^{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]hkmnlkkiigffca`][XVSQNKIEDA=;8530. ` ` 7 V V V { { { ---999EDDPPO[ZZfeeoooyyy
< < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9ae7e6e0f4234604af70b2a130f69540Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_09-41_0581 3130Seq 12301 12301_Costion_K1362_matK_R jmadera 96-Well
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KB 1.4.1.82018-04-26 11:13:04 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS |s BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ z LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS = P1AM > | ] P1RL > | W@ P1WD > | d8 P2AM > | q0 P2BA > > w P2RL > | j PBAS > > @ PBAS > > =P PCON > > T PCON > > P PDMF PDMF =4 PLOC > | JH PLOC > | C PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL SPAC AZ~ SPAC =- SPAC AZ~ SVER 4.0 SVER |h SVER , , Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH =K phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS |s BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ z LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS = P1AM > | ] P1RL > | W@ P1WD > | d8 P2AM > | q0 P2BA > > w P2RL > | j PBAS > > @ PBAS > > =P PCON > > T PCON > > P PDMF PDMF =4 PLOC > | JH PLOC > | C PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL SPAC AZ~ SPAC =- SPAC AZ~ SVER 4.0 SVER |h SVER , , Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH =K phDY big phQL c phTR phTR ~WhC0-#pPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!#%`qpScmaLy\f9LzEUJk(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfmeb%~!s]zJ:)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9]CR"lWW .C&q6X+9muy%lFLuLrtr:E5IW{k=Bb9+b{a397hVo+&k=;A{%i|o(>V&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBAr/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^i AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS |s BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ z LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS = P1AM > | ] P1RL > | W@ P1WD > | d8 P2AM > | q0 P2BA > > w P2RL > | j PBAS > > @ PBAS > > =P PCON > > T PCON > > P PDMF PDMF =4 PLOC > | JH PLOC > | C PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL SPAC AZ~ SPAC =- SPAC AZ~ SVER 4.0 SVER |h SVER , , Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH =K phDY big phQL c phTR phTR