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'  & "                              &                                         d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRETejlmlkkjiigecba]\XWTROLIFCA>;8742.```TVVz{{   ,,,999DDDOOVZZdeeoonxyy                                                                    <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5911bd7e1b704e56869f97426e6b6aa4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_08-10_05803130Seq1230012300_Costion_K1362_matK_Fjmadera96-Well K1362_H12KB_3730_POP7_BDTv3.mobSeqPop7z +AYsxkeflu ~!n$8xU?DkA^   ~  f    |    ;_{#2\S , : 5 / 5 K n < NO$[+4='FQ[eoUwv}~yfrjaXbNND 90n' txj3 N y e H ~ # q r # s H 2 I  T ` =  F vsN-j X).znV5is>8d"! 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G~H,HbwyeM4%1<EIH@3#  +8DNTTL<) '9HSVRG8(  %2<@>6* 9Tnq_M?6125;@CB>:>OqV&*Fau|udP?634420/0368;Cg8@@A2A@XAKB.bcpKB_3730_POP7_BDTv3.mobtermTAACCCGAGCCCGGGAAGTCCTCTCCTGACTAGCGTTGTTCCCAGAGATAGATTGAATTCACTTTCCCAGAGACTCCTTTTCTTTAAAGAGAAAAATCCTCTCGCTACAATTCTCTGTGGGGGACGAATTTTTTTGTTTTTTCCCTCTTCTTCTATTTCGAACATATCTCTGGAGGCTTTCTTGAGCAAACACTCTTTTTTGGAAAAAAGAAGTCTTCTAGTGGTGGGTTGTATTCATTTGAGATAAGGTACCGGTCCCTCCCAGACCATCTCCCTCATTATTTTCGACTATAGCAAGGATGATTCCGGCTCCAAAAGGAACGCTTCCTATGATGAAGAAATGAAAATATAACCTTGTCTCATTGTTGGCAGTGTAATTAAGCACTTGAGGTAGGTAACCCGAGCCCGGGAAGTCCTCTCCTGACTAGCGTTGTTCCCAGAGATAGATTGAATTCACTTTCCCAGAGACTCCTTTTCTTTAAAGAGAAAAATCCTCTCGCTACAATTCTCTGTGGGGGACGAATTTTTTTGTTTTTTCCCTCTTCTTCTATTTCGAACATATCTCTGGAGGCTTTCTTGAGCAAACACTCTTTTTTGGAAAAAAGAAGTCTTCTAGTGGTGGGTTGTATTCATTTGAGATAAGGTACCGGTCCCTCCCAGACCATCTCCCTCATTATTTTCGACTATAGCAAGGATGATTCCGGCTCCAAAAGGAACGCTTCCTATGATGAAGAAATGAAAATATAACCTTGTCTCATTGTTGGCAGTGTAATTAAGCACTTGAGGTAGG(2@OVcp"/;L\qx )3AP\fv,8DLZhr} -:ES_r~+;HUely$/9GPdox )6>MXalv ".COYcn    + 7 C N Y b n y    # / 9 D R \ k t ~   ) 6 D N ] d r }  # 0 8 C N X g x ~   ( 0 > J S d l |   ,8DM_kx!.9CS_o{.:GSap| '4AQ_lv$3>Q`o} 1@P]o{ ):GWj{(2@OVcp"/;L\qx )3AP\fv,8DLZhr} -:ES_r~+;HUely$/9GPdox )6>MXalv ".COYcn    + 7 C N Y b n y    # / 9 D R \ k t ~   ) 6 D N ] d r }  # 0 8 C N X g x ~   ( 0 > J S d l |   ,8DM_kx!.9CS_o{.:GSap| '4AQ_lv$3>Q`o} 1@P]o{ ):GWj{                                                        $           "                                                                             $           "                     %!M!:ZD +6*4*AA -R3 JaX,GO>O "9$&/5#$ 0F<$1)4Cc`GPDD/97I"<(Y.dDMa&K ( aK*C LWS'J>@R;)=7 M:.35 *`NC LZR-N?R:b 6@\IG[%Ld1AK<\_ \^QBL RN?c/## dc9+b 6 +5-<A>A\ W&*a$^VSB++RIS3/aQ O? 4Z+ b!8@V> PM-T"D]`J d H$bbA/I$4+/GPN8;;[ X]-*4DV;AM`W*%M&DG\Q1*<-J/*%\a]OJM[Q% UUWZG53I-+@ZS/CF5Bc$Y(N$X>A3DH oI3 h 6+2DbG=NGQ'-{jqpd}oX}Ht}&>+78%o]] >1']I;$\Du=_4{v%L!?mWBQ-w&n7yS4FK"d/vaN$ }[gq?D8Jmmb[ Q+I%z$Vr{I.f/?G4(GC N KI&  <zs 9`UhOU*MwVW#jwwaihE3g0d';6o4qH~}( 7C+4 HD:1LSM.  .W'!F0)2)T^ -v" q o  )3G!I:3>, !3'!],jH;~<BKy0M\N-aXFn-9HY  !;%,,n$pg~BOql5p t0^-.'n_{ q!`x%j8cd5mP&e,jBdw)m v~<6@ paM7V f&kt6p'Sf4e+yOGA8*}GZ=%{ wi|}.uz*Hp(S#8`msNl{,T %%G~Hx]= ZPS,"hx]~hue {'hY%^>,K*Wu$vlx 5~3 ) x;6" j6|u]MD/ lR|W6;dyk]p$w8+ Qy,|0|g!^hN42/,{2rN/z |K~sck PO7IBZO|d-(xy(#W o{@x{.96pWu 7;d|1,[g5[2`x_W/cjTgLszQ),+o )exvzUn>m9Ad$unVqM~l CCCGGGACAAGAAAAGGAGTGGACTTCTCTACAGCAATCGTTACAGCCACTTAAAGCAAATACCCTTGCACCTTCTTCCCCTCCCCCGAGAGGGGGCATCTGGCTCCTTTAATCAGAAATAAAGAAGTACCGCGGTCAGCCGTGTGGGCTCATACACGATGCTTCCCTGGGTCGATACCCTGGCGAGGCCATTCTCACCGAAATGGGTGCTTAAAACAGTAACTCTTTGGAATAGATACCCCAGGGTCCATGGCACTGGAAAACCCAGCCTCGAAAACACCGGCAAATAGCAGTGGGAATACGGCTTTTACTGGGGCACGCGTTCCATGCAATACGGATGGAAGCTCGTCCTTGCCCCACAGGCAACTAAGAAACTGACTTTTTAGCCGACATTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-26 09:42:14 -04:00AEPt'AEPt'APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS'BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $lHDATA $lH-rDATA $lHvJDATA $lH"DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANERLAST~~%LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMP1RLP1WDP2AM P2BA#P2RLPBAS PBAS5PCONPCONwPDMFzPDMFPLOCaPLOC KPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACA`SPACSPACA`SVER4.0SVER 'SVER,,nScal@Scan3TUBEH12Tmpr<UserphARphCH0phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt'AEPt'APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS'BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $lHDATA $lH-rDATA $lHvJDATA $lH"DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab5iFVoc5XFWO_GATCFeat..5*GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANERLAST~~%LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMP1RLP1WDP2AM P2BA#P2RLPBAS PBAS5PCONPCONwPDMFzPDMFPLOCaPLOC KPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACA`SPACSPACA`SVER4.0SVER 'SVER,,nScal@Scan3TUBEH12Tmpr<UserphARphCH0phDYbigphQLcphTRphTRn(_T+D(p_7&OMb'~6/lD-EkOptpuD:\E+mH_a2TS7}_'A\3[}k@_aA+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\dxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]4UvI{OF?jp,V0Id4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oX$^(`])>}JcVGkB*Sig]r;a4g0_[YBPxrl@;Gc+[b`[.b=jrOCy~R^bfFc':Z^xDFU)T;w>xP=O_l;mqy=Y`ge#cme/^it>QG/P.6;NJfHLW,PNWF)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc. `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"HH(Qcig=RSwsKFMrRU)>`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9}__';_/d@ikjF=mJGLbva].p>x'[IoOoh-gvxAEPt'AEPt'APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTbbC|B1PtB1PtBCTS'BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $lHDATA $lH-rDATA $lHvJDATA $lH"DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab5iFVoc5XFWO_GATCFeat..5*GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANERLAST~~%LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMP1RLP1WDP2AM P2BA#P2RLPBAS PBAS5PCONPCONwPDMFzPDMFPLOCaPLOC KPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACA`SPACSPACA`SVER4.0SVER 'SVER,,nScal@Scan3TUBEH12Tmpr<UserphARphCH0phDYbigphQLcphTRphTR