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P * 4 ) G 7 ( ' : 0 5 3 . : , + # 7 * A 2 7 + ? 7 C ; < < ? J F G ? S P @ f E R 5 2 B C & J Y M = F 4 F 1 C I 7 H " F A + 9 + / ' = ( / 4 5 / < ) ; * H 8 ? Q 7 E ? ( B 7 < J B 1 # / F 8 M 8 ? 6 < Q : 8 6 8 P 9 * D 3 R 3 ; I D > C J > 9 $ C - : % ? F C : < 0 ; = = D . 0 @ ; P : 4 @ I K 1 + 1 Q , B N = ? A C + ; ; 2 @ 4 I ; ; 9 G : = D 8 A 4 = D = I G a F F F H < O * . @ 3 L E I - L D J 6 D R A D G * , 6 C 5 = , $ > ( 5 ' $ 0 , D 5 - 3 ( 7 ) A 6 4 5 3 A > # D I : M G W ; A W P C 8 Q E / P D / I 6 C ; ] Q L H : 7 - 2 > E J ; 9 # # ( 1 2 . ? J 0 ? . 1 * I 8 B E D 3 L 2 1 4 D ) = ; 8 2 + < I 6 / O ( 8 > & @ D K N I > [ E + 8 M E , 6 7 C > C I @ 6 O T D ? S A - ' 8 + 7 3 8 < 7 + ? 8 % C 7 D : P O I 3 A I I 5 C 0 > : 1 0 E 1 = < > R 5 H / > H \ H A B > E F E T 8 G E S D _ i Q G H ? K F D 2 3 B = ? C = 3 + > G < 1 4 I 7 @ @ K 3 S I L F A ? B 9 J - E H @ B F U J U S W P R A 8 O : K L 7 A D N J @ 2 > O d Y k Y V M B J = D T - 3 " 5 4 2 - : N = ' + = ; , 8 C N D D K D < > 5 1 @ K + 6 * + : E 3 9 ? < / / M B ? G 9 F V T [ N N I W Q J \ e T L T Q X N I 4 = @ D ; 9 ) ? ; 7 H 5 < " B 5 + B L / 5 @ 9 5 8 I D 6 , 5 6 , % E ; 7 3 - 4 J 6 5 ] I 8 ; G 3 < L 8 G + I C D R V V Y K d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a C 9 E Q D > E K E E J 4 A ] d J c ; L ; B A G Q ( 3 = R F ? F N B B ; A F F 5 E H B P 6 9 L K G 6 6 D N B H L 5 1 G 5 7 M 1 A 6 2 A 2 4 2 = - 2 6 P ( ' . ; - 3 + : U B F L S R ] H d @ 5 ) Y = U < B P ] : S ? V ? ; J 2 F B J T R T R G 8 = J B J 0 L C 9 B B 0 O = A 9 6 7 < 2 4 9 ? = 2 : + G 9 3 , : 8 I [ I ? > F X 7 = ? I ? < W a S @ K 0 W O @ ; = L I 9 J @ H A I : B = A $ 8 F A 3 - 4 1 6 5 " N D ; @ F I G > > A 6 ? ? N M : - 2 = 6 A 3 J 9 > C > = : = C P Q J L T H I @ 1 9 O F O A W : D P < V Q R 5 ; S L a B R b R a c ` Y ] J _ X G Y R R O [ Y G A U A g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a U J a A K T V F > O ; C 2 & ; - ' < F = > / 7 C ( : D F 0 8 A > 5 . 0 9 , & , ' # + = 0 8 7 , % O = @ > G 0 5 1 E 2 0 8 ) ; # 4 0 * ? 8 " G < = 1 / 1 1 > 1 9 9 / 5 : & 9 % @ 9 " ? A A ) 5 N - $ ; # 1 = - $ : E 2 9 5 ( D F L 4 ? % > + $ 3 , 7 E & C M G G U a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i N = 7 ( > A E 2 W B 9 < 5 A 3 2 , ( & C . 7 ' ; 6 E * 2 > C D G F M 6 @ # , + 9 - 2 , > & 2 3 & 6 9 4 @ ' 7 A N 4 H H ? B 3 2 D > 1 J & * A / + 2 3 0 0 % $ 9 & D ( - & 9 < E > 7 A 7 H 8 G C A H > F H V = R ; G 9 O : = 4 8 7 > ) 3 > = 3 - I @ ; ? B 8 Q ? H * S 7 7 , I < 7 3 ' 3 + 2 ; 1 6 * ( " + ! ( 9 1 5 @ C " . , $ ( ) $ ; & 7 ! - . 8 9 / 8 : ' &
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 24d56a46dd4440069381a1ea4afd3c53Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_08-10_0580 3130Seq 12300 12300_Costion_K1362_matK_F jmadera 96-Well K1362_G1KB_3730_POP7_BDTv3.mobSeqPop7z"2Kq 'DmrUIY X w
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#o MO IAAAAAAAAAAAAGGAAACTTTTTTCGTAAATCTCTAAGTTTTGAGTTCTTCGGGATACGACTCCCTCCATTGACAGGGGGGTATCTCTCTTCCCTCCTTTGAAAGCAGTATTCCCCCATGTGGACCCTCGACTTGGAATGCATAGATGCTGGTCTTTGTTTGCAGGACCCTCCAATATTAGGTTAATCGCCTAATCCCGCTTAGATTAAAGTGACCCTTCATACCTCCCTCTCCCGGGAACCATTTCCCAAGCAGCTTGATGGGCACGTTGTTAGGGACAAATATCCCTAAGAACGCAAATATTAAAAGTTTGGCTAGTTAACAGAACGCCAGGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-26 09:40:58 -04:00AEPt AEPt APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +l DATA
+l DATA +l ;q DATA +l f DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ t LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS Q P1AM P P1RL P P1WD P D P2AM P P2BA P P $ P2RL P PBAS P P PBAS P P PCON P P PCON P P d PDMF y PDMF h PLOC P PLOC P $ PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL p SPAC M SPAC a SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt AEPt APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +l DATA
+l DATA +l ;q DATA +l f DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab W FVoc F FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ t LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS Q P1AM P P1RL P P1WD P D P2AM P P2BA P P $ P2RL P PBAS P P PBAS P P PCON P P PCON P P d PDMF y PDMF h PLOC P PLOC P $ PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% I SCAN 3 SMED
SMLt SMPL p SPAC M SPAC a SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE G1 Tmpr < User phAR phCH phDY big phQL c phTR phTR n(_T+D(p_7&OMb'~6/lD-EkOptpuD:\E+mH_a2TS7}_'A\3[}k@_aA+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\dxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]4UvI{OF?jp,V0Id4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oX$^(`])>}JcVGkB*Sig]r;a4g0_[YBPxrl@;Gc+[b`[.b=jrOCy~R^bfFc':Z^xDFU)T;w>xP=O_l;mqy=Y`ge#cme/^it>QG/P.6;NJf2LvQPqeR)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#R3PmZ\7rT/RD8z{q>t[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}nz:z5>vQSdW79ZYdj8WoQK5F1_;T,V2%4oNYkk?[C+3~d1bo6/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@