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"                                                                           d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRETejlmlkkjiigecba]\XWTROLIFCA>;8742.```TVVz{{   ,,,999DDDOOVZZdeeoonxyy                                                                    <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a639a533c0614ef089f18d2705032f87Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_08-10_05803130Seq1230012300_Costion_K1362_matK_Fjmadera96-WellK1362_F9KB_3730_POP7_BDTv3.mobSeqPop7zFp,z!Ut V 2 e  & / 3 2SSn9 ,MgzN0S  3jH!&-5>HR[c2jzpvEz}j~[} y`s~kbYvOE<42*e"yi r X Z InaA^d"'S_n^)a' C"$u%&u&%$#O!tpgroED  9 C<V+,? *;@2 eWVcu! @]2]Q# e \-indR{WA2,4TFL~, |^1$2>A:- /pSmP>   &]R}zKL7?/Q% -54* AfiQ>3,(#AKUx&DY\O8%-LrvB`B44=GMI9 E?[onE'       ;RZP6%Hi#DTO2plvzZC613<L`t~jP7(%->Qalpoi_SH?::<>?CLb,"[C?FLH8"PstnP8-1@Sbkt4d )=>'s+yqu)Js9KPG1iXLDADKRTK4}}qZ=" /BLI<)  %,,+1Iv T  '02+ ,;DE;* $5FXoxopwr^I815DXjwzui[LA96553.% #-/*   .9>>=>GZvZ0 6MZWG.8fk7 " *693#&?QVM9  :XigR3   .@OY[VMD<7558@L^stlnzr]H83:Nl~`MEGOYchhcZRNNSY[VK>7:H\oyudK3" -Hm~hXQRX_`YJ6%%/53*"++! 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".6984/)" !/63(       ,;IT[^[SF8,'+3<A=2# /L`bS8 "" <czY1%<HG;-',;N^ghfegkmjaWPPYgqqfR?47G[lpeP6"$3BLRSRRRRNE6$  4GQN@,.@KKB1 -781%"*-,)&'*-+#   %7BD<,  #283&  !        $+.-+&  &6DMPMF>88=DHG=.$3@GE?857?JV_aZI0EsmVMR\efaZWYaikeYMEFLRSK;' #&,8GU^\O:$ "*-./28>BA:.   %/0)  "$ !,4640,'#  !%$  &))&! #$ ','   .41% (;GJD9.&"  $-:IW^^WLB<<CLUYUH6#)B]op_C& "" #! !    !)+' "6DF<)(471#$;MUQB.    *1- %4:3"-42)  !   0>GIB3 9LOA' .BH>* 6HQK8 %<MTQJA951.'  )/-%  '.+   $$ (/.'  !(+,,,-,(-@JI@4+(,5=@>82,(#+>LND1   (>PUM8 +43(  "'%!063&  &14/$  !*.-'  %% +:@;.         085(  '("       #,.'  *6;8,  1@FF@:68<?=4% "<PWO:$ 3ENOH?6/' +0+0@MRNB0   &&   "3;9, &/331,$ 4N_`R9 .8<8/$ *02.&#.9AC>4(%'%  "<Ta_N4&7A@3 *33*  *21)    %151& !  )46/! !9O\\Q?.#"+8CHE<1++1:BEC=98?KW_^TC0"%,-& 3K^jqroi^O=*&;ShuzuhVC4-1?Tgqm[> )/,"  '+(  "0;?<3&  $+-*#   !+22* %6BHF@8/& "&% &>OUK7    ':HNH:'!&$ )1/$%% "6FLG8#';II=(1GUWN<' -AR]^VG7+&*5BLNG9* # 4BE<*   &29;6,    $5AD=/"8KX\UG6%  #,.(  #'$  ! 7LVRB+'.10+&! !#$&(+/232/+&" !"  3Lcuu`G,  &)*))('$!  #/7:71,+0=N_lqk^QF?;cA@ A ADA _KB.bcpKB_3730_POP7_BDTv3.mobtermACGGACCCCCCTGCTACCGATATGTCCTCTGCTTTGGGTGTTTTCCCAATTTATTTGAAAGCCTTTACCCCAAATCCTTTCTTTCCTAAAAAAAAAAACATTGTTCCCAAATCTATGAATGATGCAAATCTTCCCTGTTTTCCCAACAACTCTCTTACGACCTATCCCTGGACTTTCCAGGAGCATTCTTGGAGAAGGTCTCTACAAGTGTGGCCAGTCATTATAGGATCCGAGGTTCTCCGAATCCTTTTTGACCATATGTCGAAACGAGGAAAAATCATTCTGGCTTTCAAGGGGACCCCTTTCCGATGAAAAAACGGGAGTTTCAACCTGGTCAATTTCGGACATCTTATTTACACTTTGGGGTGCCACCCGCCAGGGGTCACGGACCCCCCTGCTACCGATATGTCCTCTGCTTTGGGTGTTTTCCCAATTTATTTGAAAGCCTTTACCCCAAATCCTTTCTTTCCTAAAAAAAAAAACATTGTTCCCAAATCTATGAATGATGCAAATCTTCCCTGTTTTCCCAACAACTCTCTTACGACCTATCCCTGGACTTTCCAGGAGCATTCTTGGAGAAGGTCTCTACAAGTGTGGCCAGTCATTATAGGATCCGAGGTTCTCCGAATCCTTTTTGACCATATGTCGAAACGAGGAAAAATCATTCTGGCTTTCAAGGGGACCCCTTTCCGATGAAAAAACGGGAGTTTCAACCTGGTCAATTTCGGACATCTTATTTACACTTTGGGGTGCCACCCGCCAGGGGTC!(2;DSct{ .7DUdn(2=FXju *0<IT\kz'7IR]mt,;DP^q +4@N]ew,9ISajt *2?KVdq{   + < I T d q     % 3 D O ] p  # 3 C Q ^ h v    * 8 B R \ e q {   ( 5 B M Y h t   #0<HS]iq{ $/>GQ]jw~*8BL[fo{ #/;EPZhw+7AN_ku!(2;DSct{ .7DUdn(2=FXju *0<IT\kz'7IR]mt,;DP^q +4@N]ew,9ISajt *2?KVdq{   + < I T d q     % 3 D O ] p  # 3 C Q ^ h v    * 8 B R \ e q {   ( 5 B M Y h t   #0<HS]iq{ $/>GQ]jw~*8BL[fo{ #/;EPZhw+7AN_ku                                                                                                                                                                                              3S8A?!N^QHP?/X$6@5J-V']cW8WbbMHR\"V"'!A 6L-29N=[FYI KN4>$,L7O @4?$a*EcRHLWK5(2350`P-0TJ0 :7F- _[ @^ O<DRcU,Y C+aWCPE(9P4,QV5/c,d@KL2.P.7U"`]Z#<W*<-/`L.P2BHY]c 890? =Z7]ED1a< 8))YR))# <(\ ;213AbFW/,7;7HAEP%P&^R41dM$R G@ BPS:Y" N:KHA9N\ 6: MA ,P+4LE&bH+:>.7)!b^<2#.K4=) .+ 4Cd IP@'"AJ<04FGVSJ FS [ ^k& AUv  s ;b$B/y$N]ej"pL{pm:Y~X_4Dd8L+Wzs9j/15mqmGU8ZjRFN6\8?<4h}04('S;n0vmx]=iy!NTMHwT=4Wu,d[t[;j/ r[xq' >) &fW'iNi8Q7-,$nSKg^8=5/:?B~ qej,2dR@Nw>"  W68xM:W*([g'x./;86A;~Lk A=MV'@ZEF%%3: 6+!" D  %4vz!6# .#fO; =B,&R$531+*L  PJdB0k<  >5'/0{4*U! W 9"2/cC8`dWbv <$1Y g^c[YiO,A-\${Y&N9rCdE{T "M8W[? rp8)fd(P&;[4`+|088^xE" 97mjIp^&H}S,/y# );`E($(>6yKG\t+u-`D6|7.RvmI,c(LXtGHji0jVn$q Qi$BQp&|4m@3HUC/ B G]$#D(B   Nx.o"#.ZzBg|eXz)~0r-c CLk:vH5F F\VlYiteEv udzA5iHo8O"jm2X[ovNvpP)%4o&pTEm|K.@}`uR8LP6f6{ rSg4C(,ytL]"FGjFkj#)mS^>so7pQK/90k H.2O7GGAATAAAGAAGAGGGAAAGGGATCTTCACCTCAACAAATCCCCTAGGTGAAGACCATGGTGTCCATATAACGGATTCAATCCCTTCGCGGGGGCTGTATACAGGAGATTTATCCGTTGAAGATGGTGGAGGTTTCTGACCTTTTTAGGTCTCTAATAATAAACATTTCCAGTACGTGGCAGATTCCTCAAAGATTTAAAATCGATCAACACTGGTAAGTACCGGCTTCGTATTTGCTCAAAGGAAGCCCCAAGTACATATGTTTTTTCGACGGGGGCACGCTACTGCGCACTAACAGTAGGACATTTGGTGGCGGGATAAGAAGATTCTTACCGGGTACCTTTCACATGATAAATTTTGCCTTTTCCTGCGGTAGACAAAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-26 09:42:02 -04:00AEPt-AEPt-APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSMBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )[RDATA )[R7ODATA )[RDATA )[RܻDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEELAST~~L,LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS/yP1AM>P1RL;P1WDAP2AMGP2BAJP2RLDPBAS1,PBAS/PCON:,PCON8PDMFyPDMF/PLOC5PLOC2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%/qSCAN3SMED SMLtSMPL pSPACA[rSPAC/SPACA[rSVER4.0SVER MSVER,,nScal@Scan3TUBEF9Tmpr<UserphARphCH/phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt-AEPt-APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSMBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )[RDATA )[R7ODATA )[RDATA )[RܻDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab\FVoc[FWO_GATCFeat..[GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEELAST~~L,LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS/yP1AM>P1RL;P1WDAP2AMGP2BAJP2RLDPBAS1,PBAS/PCON:,PCON8PDMFyPDMF/PLOC5PLOC2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%/qSCAN3SMED SMLtSMPL pSPACA[rSPAC/SPACA[rSVER4.0SVER MSVER,,nScal@Scan3TUBEF9Tmpr<UserphARphCH/phDYbigphQLcphTRphTR&BQ{6Q2\gDT]U'Q>lW+UfyKnY18ds]`?Ne4sX$9WK]}DV&[>:)II;?1EFiRCwh1nk-~4Kb}wLD7X{u./uX8?;pPJwiO*$]_`#?QI}yn%/k%S>p=6"4#OvR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;8)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lsXW}0{vMa`HUX;FEX[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9uoW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'t*?_:xslBEBuECcO58QiyZ$wpor9Sqwg5j*"}c:(fQ;42:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NudrVLgJRv)1mfc4hApvaUGP1RL;P1WDAP2AMGP2BAJP2RLDPBAS1,PBAS/PCON:,PCON8PDMFyPDMF/PLOC5PLOC2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%/qSCAN3SMED SMLtSMPL pSPACA[rSPAC/SPACA[rSVER4.0SVER MSVER,,nScal@Scan3TUBEF9Tmpr<UserphARphCH/phDYbigphQLcphTRphTR