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"                         d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRETejlmlkkjiigecba]\XWTROLIFCA>;8742.```TVVz{{   ,,,999DDDOOVZZdeeoonxyy                                                                    <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5a4adc5502ef4251ad86280ba3c78abbSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_08-10_05803130Seq1230012300_Costion_K1362_matK_Fjmadera96-WellK1362_A8KB_3730_POP7_BDTv3.mobSeqPop7z !! !#%')*))))*+++++,.13553/,**,/356531/.,++**)(%""%)/6>GQ[fu %=VolQ6vdWOKJJHFB?=<<=??@@@@AABBCEGJNQUY^fr@K>Phk*_rc44`'7><2$ YsB=oJri<BsaTKD@>==>ADJQ[h{c8sr.OiTF?>BISd~,3TKQ-m;fgD0%!"!    8kDO_6M#    6^#qZ ~L(  ""! 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""&"4"@"Y"f"r"""""""""""####,#8#E#Q#_#l#x###########$ $$&$4$A$L$X$f$s$$$$$$$$$$$$%%%%,%9%E%Q%^%j%x%%%%%%%%%%%& &&"&-&C&M&Y&d&p&x&&&&&&&&&&&& 2<L_ls $28FR_ly&3>HVdq~*4>KValx&1>IT_ly+6@LYfr +6CP[ht'2>JWcp| !.;GS_kv  - 8 E O [ f r |    $ 0 = I U ` k w   , 8 D P \ h t    % 1 = J W b n y   ! , 7 C N Z e q } &0=IUbnz ".:EQ^ht(4@KWdoz+8DP\iu $1=IUamy*4@LXdo{ !.8DP\hu)5AMWco{ $0;GS^kw*7CO[fq} %2>JUaly ".:FQ]jv+7DO]jv(5BMWep} &2?KWbo| %1>IVco|&1=JUbn| #/=IWcp}    ' 4 @ M Z f q  !!!"!.!;!H!S!`!l!y!!!!!!!!!!"" ""&"4"@"Y"f"r"""""""""""####,#8#E#Q#_#l#x###########$ $$&$4$A$L$X$f$s$$$$$$$$$$$$%%%%,%9%E%Q%^%j%x%%%%%%%%%%%& &&"&-&C&M&Y&d&p&x&&&&&&&&&&&&   -'52:6466>>> />//>>>*>>>>>>.111>;+>+>>;>;;>>>>>>>>;>3>>>>>>>>;>>>>;;>>>>>>>>;;>>>>;.>>>>>>60>>>>>>>>>>;;>16>>>>>>>>>>>>>>>>>>>>>>>>>;>>;>>>>2>>>>>;>>>>>1>>>>>>>>>>>>666>;">>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>;>>>6666>6>>>>>>>>>>.61>>>>>>>>66>>>>16>>>>>>;>>>>>>>>>>>>>>>>>>>;;>>>;>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>;>>>>>>>>>>>>>>>;;>>;>>>>>>>>>>>>>>>>>>>>11>>>>1>>>>>>>>>>>>>>>>>>>-&;8."28>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>+ .>>>>>>>>>>>>>>8>>>>>>>>>>>8>>>>>>>88>>>>>>>6>>>> 1>>>>>>>>>>>>>>>6!>:.:::::::::664:::6::(66:*:4:.6/:*6666:66::::6:2:45:2:'/:3::) 5*4::':6:'!0:'1$12::,1.::+,12::3::::2663254:1,66234)2./'532##!2(3.5 .!!&$,51*145((, *5#4&*"/)'!)/---*+- !-**33**& ++*"%0.* .00../..,)$/' .0 &   &    -'52:6466>>> />//>>>*>>>>>>.111>;+>+>>;>;;>>>>>>>>;>3>>>>>>>>;>>>>;;>>>>>>>>;;>>>>;.>>>>>>60>>>>>>>>>>;;>16>>>>>>>>>>>>>>>>>>>>>>>>>;>>;>>>>2>>>>>;>>>>>1>>>>>>>>>>>>666>;">>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>;>>>6666>6>>>>>>>>>>.61>>>>>>>>66>>>>16>>>>>>;>>>>>>>>>>>>>>>>>>>;;>>>;>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>;>>>>>>>>>>>>>>>;;>>;>>>>>>>>>>>>>>>>>>>>11>>>>1>>>>>>>>>>>>>>>>>>>-&;8."28>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>+ .>>>>>>>>>>>>>>8>>>>>>>>>>>8>>>>>>>88>>>>>>>6>>>> 1>>>>>>>>>>>>>>>6!>:.:::::::::664:::6::(66:*:4:.6/:*6666:66::::6:2:45:2:'/:3::) 5*4::':6:'!0:'1$12::,1.::+,12::3::::2663254:1,66234)2./'532##!2(3.5 .!!&$,51*145((, *5#4&*"/)'!)/---*+- !-**33**& ++*"%0.* .00../..,)$/' .0 &   & )@K"(P#UT)SDL;`ZC%"PJSF5;DP6E$)U:/ZNY& "DW+VDE6IL\)=6F;$JCB %&E>LK^=JB%N#B(=,[#Rc=45E WHF_AW6)W#-*N'7 8NN.B%H,1c&FT&)C0H@2DO3RF,*?%QX Z( N %'AQ0W;F<'\M+<5C@I:#0X7'!G:DL .B%- 2>>IEED>QOMK&6 > ]QF72_=`80&%BB".WE&0Z$WY E](7 @ E0KZ(  67%;'%Ka^W$Z^\@(.'LCb0`!K&J\`7-\  @H%>/- 2?P@,322\D$ U@,*EQ:AQb M!)B'<T*R AOM\ &M35HL$K`_]^c>(8U :H$ N)N+S `9!6 d7-7S Z&"N=@JF:!O -; 1L]/8D$McJ]<3[<C 7OaIG < ]!I YUL Y5$:IV+Fa "[ OTSF)"*+^\#'!"RW!9,^,0& 2M,UaH7G)_VL'.Q*NN'@=3;.=G3Pa1)W-A%:` !V 7NQQ].d\W4D c JZD'EKWNQ7bBIT30_ @aT8EaU>1$'05bB13C-T9^+)AA,9-O+VU)Y=]J4V`_/<]9X H%@ `2^MW \SLBJ(B"cB )%RB"[I1T3\dX .2JD?:5/*% ?CB2"a\5R;4J228dJN22B :2`2'"2 0`|0)"Zo0dvb0MXuPy-vkT`CZ+>os8e lx M&]N4C4?s*Sw{Z02FQn]T2 J M&Ma0tB$B h6wDldBMeobH7s[!CPLokuSZ|uh* g=Mu][+S(]:v+>C3% IbrcGMZ%3hhP#hh'm#kat@9##>%k k0~}{>)sz6~&*VMn|u bdL 2+b?C)Qf'>7, i]YAl]DR BB6L! $L@@ *RL CSv@KOJx}^ A@Jg ]\=&<XNa^ <]^ "r7v\XT]edi0Ts'R>W:fBx~"]N8E7Z1+ ;g>Ny XR+W8B_}|&GTO {lsW-ysjma 5 : [  DYU8N '_nbp\Zf^w*r Iu   O |D,<Jy^dd\I7h>)GU3x.'VA  5#%"          xxx                ,4 ,/-"!2!8)*>+*%.-!0:(7>+)H2=3>-5A*&#,9223B6-A>mE4=9?;K:/?JJt:65=>.3H<=:G?2@AEK>@D]B.MEBJAaT\OEWiSDUOMRtc_\~\WZ[XXbP`fff`ngpae]_{uc`)nm}nwq}{quzzzs}4y}D4=vyM#$i `k  ua PE])JYppM^E8Lxpp4'r^t,p3;i2,ud@_ayp."Asr-@]6,I8s^HMZG8ZBTzdBQpA^Y(E8Lr[c]4dp!F ?dn%mo(8Iw'0 ,H ^l-T^f~3Z&p4GEpgv@=vkK8Yu`HhL5q5[bXmepBWp{Dfrvc&p=I:<pppp>pk0zppp3ZGbptp[2SiS_Qq16rk:aqJRJZ\6@& ]g i~5+_('X!W2 +!au#C/)) &  !*FI* IW%'0%C#!,{  5z#!$" 3!4(>  ;!(3+%#8-B+3 =V/W(63%EL!+#)$5E,  $%( G-< 1 1$ '$ %$( (&   )( !)8- %   $ ,+( 2*.I#9!,;")1!)  !$#   00Q 'R#!-  )&,*#L('24#&2+*E)9'!M>M##*V)-%e7 v'' L""#.!<3+4.)35]8V$0#N>gQ S+Mu3TL7aDC6<3GMd2MIUSY,=|<>/=IUR8VQ=C{wOC,Te b;O1 4YcFQA^E\>XxAZ|eMAG>kdWhmk L/Rrms7rENhWO I yjXc5= kbf0O1:p@z1 CTACTTCTCGAAGTACGCGATACGTCTCCTCCGCACAAATTACACCGCTCCACCTCCGCGAATAATATGTGCAGTAGTCTCCTAATGACACCGAGGACTCGTGCTTCCCCCCCACCGAGAACTATACGAGGGTGCTCGTGCTCATATAGGACTCACGAAGACGCGGAGTCGGATTCCCTCCTCAAACTCTCTCCGAACGATCCTCTCGAACGAGAACGACATCCTCTCAGAAAGGGGGAGAGCGGGTCTACCAATGAAGGGGTAGAGAACCTCGCGCCGGTATCCGCTAGGGAGAACTCAAATACCTTCTAATGATAGAGGGAGATTCCCAGGCTCAATGTGAGATCCTTCGAAGGGTCTTTACCGTTTCCTCCGGAGACTCTCCTCCTCAGAGGTATGGAAAGGTAAAATCTCGAGATGAAAGGAGTTTGTGTTAATAATACGCGATGTCACCTTCAGGGAAAGTCTATATGGTGAGAAGAATTCATCAATCTCTCCTCCCAGAAATATATCCAACGAGAGCTAGAATGTACTCCTCAGTTGTGCAGAGTCCGATGGTAGCACACTTGAATATTAGATATTATGATATGGAGGCACGCTACACTATTACTTCTAATACTTCCGTTAGTATAACAGTCGCGGTATCGGTAAAGTGTCACACAGTCCGATATTACAATAATGATTCCACGTACGCATAAGTCGCATACTCATATAATATACAGCTAGTGCGCATATATAGAATTCTCACTCACAATAATATAAAATATTCTCTCGGGGTGGAGAGCATATATCTTATGTAATATATACTGACCCCCCCTCGTAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-26 09:41:57 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSuBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *HTDATA *HT9)DATA *HTDATA *HTIDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~t<LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS6P1AM8pWDP1RL8pPP1WD8p]P2AM8pjP2BA88qP2RL8pd$PBAS88:LPBAS887PCON88MPCON88JdPDMFyPDMF6PLOC8pCPLOC8p=PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%6SCAN3SMED SMLtSMPL pSPACAZSPAC6SPACAZSVER4.0SVER uSVER,,nScal@Scan3TUBEA8Tmpr<UserphARphCH7phDYbigphQLcphTRphTR%This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSuBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *HTDATA *HT9)DATA *HTDATA *HTIDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab FVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~t<LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS6P1AM8pWDP1RL8pPP1WD8p]P2AM8pjP2BA88qP2RL8pd$PBAS88:LPBAS887PCON88MPCON88JdPDMFyPDMF6PLOC8pCPLOC8p=PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%6SCAN3SMED SMLtSMPL pSPACAZSPAC6SPACAZSVER4.0SVER uSVER,,nScal@Scan3TUBEA8Tmpr<UserphARphCH7phDYbigphQLcphTRphTR6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGUNMCp:C2OlM'AQ)yA.OyTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"HH(QgxR5QJb^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0oW~!Te4;,: TZc:'?tgbmt\j!+h5A450p$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VD*p;I:/wEkfptir"8|oTq}A|1D@<7>{AXG9{$5t{dV&yGpc6QP!gFS]T'_C:rF(5,f *"