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E Q N M I S 7 W _ J ` U J P K M X O S @ D A ; X M P K @ N C Z N B E O O P E X J L H H \ @ 7 H @ F ] Y _ E I G R 6 > 6 ; G J 7 J ? > @ 5 P G F C : 6 A L G T 9 Q G C < R O 9 @ D 5 = @ F > B B < I E M < M < I Q M L D I = A J 9 9 I 7 5 B : F A X E @ F M E I F < G B P 0 V S ? H 7 G [ 4 B A V E U H H P T X W ? P T S Z K b Z O I A = A O A G H H @ E N E P > > N P H S > ? I G G L I P a H V S W V K Z ^ ; P P G M A M G D I I K C L K R Y ^ T > N K 7 6 H = K ? = 4 > A < < < H < H E > ; F ; A ; 7 3 8 A < ? H A E N E 1 : > 1 5 9 8 G 4 A < > 4 > 6 @ 6 4 A A 5 7 A 9 5 ; 6 8 6 E A 7 B J I < ? > E < F ; S X S N H M B J U C I C S Q @ C P I @ H \ U Z Z U X T Q P N U M V ? F J . < A B > G A 8 9 D > 7 A 9 < 9 6 ? M : B B M F L D J @ M W P ? J P L J D O L P U L E B E F F 9 C B M 8 < 4 4 6 6 R F 8 D < 3 < E / 6 : ? > > ; 9 : 9 8 6 D - ? ; < @ H M B E M A Q M R > R G H C X J K N R ] W S K G M > L J S ? A < O 9 M [ > D U N Y W Y S T S P \ U @ T D K H @ N H N L ? ` W J C H E 5 9 G O = K R D M B 5 O D ? 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< < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 12f0cf7edcc54441af40af4aceb99843Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_08-10_0580 3130Seq 12300 12300_Costion_K1362_matK_F jmadera 96-Well K1362_A7KB_3730_POP7_BDTv3.mobSeqPop7z,]wb& /%
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OBM !tV b w b T9# ? k' n :g [ @qL:5 /L =l Ux@? ;]+ Sn ~1ACCCCCCACCCTCCTTTCGGGTTAGATAAATGCTCCCCTCTATGACAATAAGTAGACCCCCCTGACGAGAGTGGTCGCCTTTGATTTCTCAACGTCCGAGGACTCGGGATTCCCCTCTCTGATGAGTGAAAAATGGGCCCTTGCCTTCCCTAGAATCCTGAATGAGGTGGTAGGAAACGCGTCCTGTAAATCTCGAGAAAGACCTCCTAATAACATATACCTTCTTAGGTCCCTGGGAGCCATAACAATCACTGGGTTTAGACATCCCAGAAGCCCTGCCCTTTGGACCTCAAATATTATTTTATACAACGCACAACCATCGCCTTGTAGCCTGTTATAAGAAAGCTGGAAACAAGTGTCAATTGATGACTTCGTAATTTGTTGCGAACAGTAGACCCTCTACGGTGCGGCGCTAACGGTCGTAGAAAAATTATCAATGACAACTATACACCTCAGAAAGGATTCATTGGGCCTTTATCCTGATGGGATTGGACCCAGTTGTTGTCCTCATTCATCCGAAAGAAAACGAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-26 09:41:56 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS c BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U :' DATA * U DATA * U C DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ bu LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS : P1AM & O P1RL & K P1WD & S P2AM & \< P2BA `b P2RL & X PBAS = PBAS ; PCON I PCON G~ PDMF y PDMF : PLOC & CX PLOC & ?2 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% : SCAN 3 SMED
SMLt SMPL p SPAC A[ SPAC : SPAC A[ SVER 4.0 SVER c SVER , , n Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH ; phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS c BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U :' DATA * U DATA * U C DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab rX FVoc rG FWO_ GATC Feat . . r GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ bu LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS : P1AM & O P1RL & K P1WD & S P2AM & \< P2BA `b P2RL & X PBAS = PBAS ; PCON I PCON G~ PDMF y PDMF : PLOC & CX PLOC & ?2 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% : SCAN 3 SMED
SMLt SMPL p SPAC A[ SPAC : SPAC A[ SVER 4.0 SVER c SVER , , n Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH ; phDY big phQL c phTR phTR z^4?FVwoyljyK{t61h67A"yV+XZ{8+;N}?"\=QtAN-GQ6)l4;qr-.^_Nxq^*>H~?p96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+,%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KKVd~65nbJEZG59F~q,/5`w=yBimJEIoM/V,<|ag&<{[;NH(- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JUx2ffU"&6,2SayX,8JThnbi2dP?l~Wo]yi-IX/TIIgsR8&BmrY\&OIefaqQl'[)VBTd.u?z:6R;B*#k@l'Z9dEez x:H-p@~KCozPk&OE6OLiE8nZDK AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT ` ` k B1Pt B1Pt BCTS c BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA * U DATA
* U :' DATA * U DATA * U C DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab rX FVoc rG FWO_ GATC Feat . . r GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ? LAST ~ ~ bu LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS : P1AM & O P1RL & K P1WD & S P2AM & \< P2BA `b P2RL & X PBAS = PBAS ; PCON I PCON G~ PDMF y PDMF : PLOC & CX PLOC & ?2 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% : SCAN 3 SMED
SMLt SMPL p SPAC A[ SPAC : SPAC A[ SVER 4.0 SVER c SVER , , n Scal @ Scan 3 TUBE A7 Tmpr < User phAR phCH ; phDY big phQL c phTR phTR