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H C L E F L O D S N B Q L e d W S N Y ] f p ^ g d h h _ ` [ V P ] \ Z s c l i o a j _ R a Y [ L L M U G F O P P O ` U j r j t s g r r g e [ H N Q M Z U a Y d S U e m g k z g b g d f h d ^ K J X V ^ R Y d P X O a \ Z f d b t i w w i k f k Z [ W R j i g R N [ P Z g W e { z j j p k V R a G U L F J N X b ^ [ h ^ g ^ d S f _ X M V O Z K O I P P J I N Z O c [ e ` p \ ^ ^ c d X Y ] d T P L R D O E < I O N < I ; : O U M F E X E N U Q C I 8 Q = H V O R P M @ W ] R L H K M L S U ; S G F a G Z G ? K 6 > B H C @ O C P = I O R F b Z i [ ^ X O Z M N B b W s d f m w f f d _ X g i _ w l y y v } } g Z [ { t l | y ] i h h d f [ f W l c i h ^ _ O b d k [ i q i x } y g s t ` b U b V c I K _ b J n Z Y M ] J X P @ C K < L X W W I Q U U A : [ L 7 R ? H O C 5 A @ ? F O J K C L S V O i Y a d ^ d k W O l _ R D O > 6 R E [ M M K F M J V L H Y k k p ~ t k p r l j ` c N a F P G 2 P Y H ? T . 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S : 6 1 L 6 ; F B , : < > > 7 B M 7 4 1 7 : 6 = Z H S T L e h P c _ T C U J P ? @ K B P S @ M F 6 B A G C M G P > M I K Y R E S S Y ^ M ; R ? 6 = H F P S D ; U a L \ W Z ] Q D E P ` O C = I < C H K < D B V R ^ P b a W Y ^ Z h J S O [ a ^ r X i m i b v ` c Y e Z I b ] ^ U a \ O _ a ` X Y Q b O c P Z a W k [ s u i o a n p k o g r u a a s a k ^ e ] q ` W o Z \ o f b l l P v Y X R I F H Z D M E [ T ^ Y G N K M _ I U _ _ j s c X _ Z \ G ` a K e X M U [ ] U S J J U T U F I L T Q Q B S T S R K M U N . P A 9 5 F G U R b I M ] O N O B @ H < Q L X O I A V E > = O B I 3 H N Q C K E I K E F ; ; @ H K B < G ? F K F _ N N R E > P U Z c Q f i p k e f Q V P W Y Q _ \ p e i Y n T ] b R c H ^ G _ ` b U a i b n b k e d l x } m s t z y q o p c t ] ] a T Z b [ A Q Q T T L K d O e [ \ ^ _ n W b ` i a O ` X O O B P N @ A = C ? A C G M M H D ] T a ] T ] O d R N N P F T E ; ; H 7 ? I C B 9 0 : N 3 2 M F D 9 K R I F E L = 6 9 . ; D ? 9 E K < I E G E U M K S T X O D M Q T ? O @ < < C > @ L J [ 1 Q H A H E b Z L M < N ? > E E D H : , F @ < ; 5 C 8 3 ; > 4 A : = 6 ; @ 7 = 6 9 ; 9 D ; ; / @ C F ? : @ > I : ? A ( C 4 ' = D < < 8 : L C > G H J D O : J J < P > Q I @ O d L 2 > O > 9 @ 9 < G L 4 H 1 7 H C J _ W Z a d f d k t b c l U V T M Z F ] ] [ b j ` T H P Y P M F F W M V Z _ f a c Z j g d j i j h i c h m Z d t p m j p T \ V \ L I Q N E = P ? A D H 8 R T ; A K > Q @ H L M H Z 3 R N @ @ 3 1 O E < N B O M < E I K O M L T E Y G V M G I R T S P D @ A C 9 E < H 5 F O 8 8 M F C F B F C 4 @ 5 6 + < A M 7 C = R R J U W I R O V K Q B C P N M ; C H ] @ > 5 @ F 7 6 B 9 = > 8 E W @ @ K D ? ? R E < 5 C C F 7 @ G ' 5 < 0 C 9 1 B = 0 . = 9 > ; / 8 5 E A B I D I T S M 0 A ? B E = : 3 0 0 ; & 8 2 / 4 4 9 1 < ; 4 " 7 . 9 2 2 F = 3 < 6 . % 2 0 0 ; 4 P 8 ; < I H T M I W C H 1 ; 6 = 3 1 A < E B H 9 & = F - 3 B 0 = C 5 6 5 = D C @ B A C 9 1 A E 1 7 3 > H N 4 F = = < J S J U ` Z f ^ \ U O q s s u r | w l r c Z F g c L ^ C F X E G \ V M Z L b e n t o d d h d W d p Y b s o z w t g j d Z U m e R [ V b V ] J W M M g ` P G H C U H S I F V N F U E Z V T 5 G : J L L M P P J < U L B P W V M H 9 B C L P E F k ] Q f a ` c k f e e c ` [ l c ` [ U T d N P N J ] < B H [ G J 6 H @ F @ < ? E H > b V G N O Y \ g f Z X b Z h O f ` L F T W E ? M E K P M @ M 3 E < D O 7 K V \ C Z X b S Q d a M O F 4 V D 8 P N 8 @ 6 A > / 9 2 D 8 > N B = @ 3 0 L @ $ = 9 7 G F G D F K K _ V _ F : U F G - < < C I . 7 0 L E R G < O 8 ? E 7 < > < B > E G B H T I \ h \ h i S Z Y X M ] U L S G I 8 A C B I K S G ? 8 N @ @ 3 > 6 K C 9 K < = M : K ? G D P O U [ ] g W h R W b \ e Q V c T K H ^ K L V B Y ] V a x k b M f [ i ` Z R H ; M J O A K W C J G H W F : G K > ; D P G 2 < H P B L T L K B H N T ] M N J e ^ Z N W n K T L V A S P G 5 G ? K @ 7 R 5 J C D G ? C F : J N U G L L W L J S S G V R X H R B P \ > R E Y R O Y K V M ` a T V N X U H R f f d _ n v j e \ j K \ R X S S a K U [ \ M K P K V A C < ? > > B B I G D J D 3 ; A E ? < D R B = B L I L > B P : G J 6 < > 4 M M A = ? H B A ; D < 6 8 2 L ; F E K J T c S h Y X Z e A V L J V L E ] K > ? L F ? C 6 1 = 7 , 8 . ? = : 2 1 < % 5 9 C = ; C F A B C ? X < H E A D E B 4 @ 8 D @ T E D D O = 7 @ B K @ A O < B H K D < = D H K ; G M > T T J R G D A N D C Q Z V K R J R O G X I V K c L X O T D c T Q [ e V j u d s l p } k o j b Z L T O H D \ G > N L J B L F ; M N > V [ G F Y ` U k c S ` i d f K i j ? [ l b ` ` s g c R X I U F F W I C @ E L C O U O H W D B O \ [ L C L [ _ R \ S 9 V R : U H Z M N Z M A U b U R R N ] E T ] d j m j s h | h p p p f ] R T e Q P V g Y U K O S H @ < 8 = N A ' ? O I D ; ; ; J G M \ H d ^ Y J V ^ [ T K J e F P F [ > F ? E J < < C + ; C 3 = A + 7 ? = 1 3 ; A ( I O 4 1 H O E . < # 8 2 - @ A 6 ; C - A 0 9 ; > 8 3 @ 8 B 7 N < E J ? 0 ( C 7 9 1 C F C 9 5 ( = < 9 5 > 4 6 F K ) D H I C : 9 @ < ? ; : = ? - ; E K F = @ 2 8 A 6 C D J @ 1 8 K * / 9 5 7 = 6 4 8 ? 9 , = @ 5 9 9 B B 6 D 2 3 7 ? N T H L ? 2 7 K . < D 5 * 2 F 2 = 3 / - ' * 6 ) # % + ( . , $ 3 5 7 7 ) 3 , 5 ' + / F 0 9 ( $ ) ' , 8 - ( ; 0 - 0 5 7 - = . = . = C 1 K : 9 , * ? / 3 / 6 , 3 - . $ 6 G 1 6 2 , 1 1 5 3 6 < 5 8 / . K - 1 = F A ; ? O : ? A C @ D > ' : J ( 2 ? ? 6 7 8 4 1 D 9 6 > 6 A > @ < 9 9 D ; E 7 = + 1 3 - 9 B D @ 3 M G J L B P Q X A G F = J 7 : O : 7 L A 5 A 9 @ B 3 : B A > : % 7 3 . 6 ; D < > D H ; 5 6 = E A E ; B > ? J t H Y R J M O @ Y @ O E O 5 W O R W _ W Z T V Q [ ^ P S C J N F J ? V I S J O L A M O Y X V R U V B S R R M F T E @ 8 K E 4 H = A : < @ - C A F A T F L G R H O Q H ? [ B R R ^ O R F I J S T U 2 < @ / 9 5 : 6 3 F ; % 8 ; A ? ? : 1 1 2 A 5 5 ? D F L W I : V G R K C F E I E N 1 5 > ? C B = 4 9 4 C B 8 9 > < G M F O P L R B P F J J A J M P R Y S X V Y O L K R B F D Y N A G J ^ c a P X K K H H B E K T ] T F G K D @ 5 = A : @ 8 . G 8 8 @ K D ? ? F 8 2 L . A 4 > B 9 A > : > > A L > G < C N B A C B ; S T I M > F = H 9 = & 5 / 9 ( 9 5 < 9 < 0 A 5 H 8 ? 2 : 3 H 2 0 B - K ; : 7 < / ; 9 J A < > A = B O A ? ? A 2 B 1 M ; F F E J V Y R b N G I S W X Q b l m h Q a V b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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4546e5438a19428aba5f249763389896Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-25_20-27_0573 3130Seq 12288 12288_Costion_K1361_matK_R jmadera 96-Well K1361_C10_RKB_3730_POP7_BDTv3.mobSeqPop7z + M Pihabm7e# +f-?'z> @bc=@2PR2%%pmTwzdA 9k#JYQ6
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KB 1.4.1.82018-04-25 21:59:34 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA #n F DATA
#n F + DATA #n F r| DATA #n F X DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE L LAST ~ ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS < P1AM * A P1RL *
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RUNT 6 RUNT : RUNT 3 RUNT 97 Rate RunN " " S/N% 4 SCAN 3 SMED
SMLt SMPL SPAC M SPAC L SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C10 Tmpr < User phAR phCH j phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA #n F DATA
#n F + DATA #n F r| DATA #n F X DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab .a FVoc .P FWO_ GATC Feat . . ." GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE L LAST ~ ~ ~ LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS < P1AM * A P1RL *
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RUND
RUND
RUND
RUNT 6 RUNT : RUNT 3 RUNT 97 Rate RunN " " S/N% 4 SCAN 3 SMED
SMLt SMPL SPAC M SPAC L SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C10 Tmpr < User phAR phCH j phDY big phQL c phTR phTR K2OI*-V>yj6m}:)9`[?y[nvbeK]TM7l:2Fj62o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbV_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKM\8}48vc. 7$?Z2o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*M0JNbm|XMrDK"*.GHiJ(/\_'MChMoG;KMJ95nM,aVh6dYHp-Op;ZJLgS]jTj.uFk4W7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYCBO(TMq:/9R(6iWFUFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WeUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4r;Dr.=:xOYPHwZrmV.*q_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|G?G9EgY%QWd(eu+wGkSOj!Q4X+*flVinJils>:4L\\W'KFxz2jA&owbBV*d> AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT f f