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",,III^^^{{{&&&999DDDXXaaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRD]eghihhhfedca_]\ZXUSQNKIFC@>;8731/Y```VVV{{|   ,,,999DDDOOO[[Zeeeoooyyy                                                                               <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8212ac5428e149dcb2d973ea997765d5Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_20-27_05733130Seq1228812288_Costion_K1361_matK_Rjmadera96-Well K1361_B4_RKB_3730_POP7_BDTv3.mobSeqPop7z &0=Ne}-<L\kv|zsg[NC8- |^B)umhe`YOD:3.-./00.,**+/5<CIKKHEB@?>=<:989:;;:851-)&%&(+,,(# ""  #%(*,-/0246652/-.0354/)" )>Xt :N\`\Q@)qcUH9+  %8Lc O<ZkphQ+g}bTOR]o8nukju;lrJz,Xv3f2,r - I 6 L1,y^OIHIHEA>=>?=91'  .M{1NL~y>MY0sB) !     +VZdP|(,&n7   #&((&"!Hg67(T; .W7#B=yv>   >WL{&k1!`LzF\7]7[   9?"6\l8m  JFI%+fV  0bJ`$Phyy,l8q^v)C   KF(rk%jAXS4wOL.lZf X!        8qBgiH ~D   A(_V]  ?I}Q@  $(((%  b2)5_ .  !.:A@7%L^5yS;N L+'jl.:Z "$&)++*($    )*a PTh?t69J!!&!    9@  "  )B.0ILcAlKXU Rw p.ZaDO +1;LdzvY?IFLnIO  ")2>KW`ed^SF7'  #L  _/ )4=A?8.%    V@#I)M` UR   X%1ta          !Z M Fm! 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#*($.'B/+('%'41*2 - *5!3=4&1,!>:.19"=O;-)(,>23$#*9J[51NRCG7LA8:<Kf+6[AQU8;0A<W@ooTL@cCKLJFDSLWMPZcDcPOVXZET_HOQTCfLPkbqMvgf\gb`_rlqsXq{{oy\cj]xpjvp~]}`~|1.UB<D#RUb/> +!u 33933  &       +(UU 4U*U Uv#GPGR!5GGUUGGG-BGGWGGUUUfXooWL=GGGGGZZGGG!GUU[GG/.GGUUGU F,Qp6PW#tpwl|?mp*p-42NNVY=, ([ q3+Jv Up$Y,,uY`\X9sOp, idpwn1m58X ~dNH67R@ zYUw |5?pX9VXB$?ddu55j2 ^k8Ldt\LdVd/tp9ppUd1 85ptNF[pmx toZ>dC?pg~<pprV{hpsppwp}8pp3,rm pppqppLppdpdppA'XG.R)e+R4$<|MM8(cI9,T(}!)15+]'2nD>J?dM yYdf}w~M=%-7!+ " @2 5"5 *!**2#% 17+  *4!% "45 '$PB37#/$#<)+'+  4(  % %)&!"$("  )<#!)5H--3K%('%$4' %!'(!% $# &313'82!&" 6&'%0!-(%"&"7*$!&&f$.,&  ("!#M39&)&/#! :[0(,.-  ?6)"% "&+!"- 7BY$7 30&+5*":)H^,6281*0Hi(/C7&7//A6/\5I?Q%:W4;[2 ,d ) d(97t!jN9A5L436w'.F`X\V> 7(/GW1-90XqO7"'\a =bm .Nd5>RV121>Sl}xgZ'kH)Fgzus>trkROFT9"r;{Uzxo_OzmaL}Gof{-!K9LtYl_~ Kv]fML "' M DP^&G 6/ ].HN"CATATTAATTCTCATTGGTTGGGAATCTCCTTACTTCCCTCTTCTCAGGTAAACAAACAGCGACTTACACGCATTCTTTAAAGCTCTATCAGGAAAAGACCTATAGTAAATCTTTGATCTAAATTTCGGTCGAACACCGTACCTCTCCAGCGTCCGAGCTTCCATCAGTAATATTGGTAGGTCGGTTGTCTCCCTCCTCATGAGAACGCTCTCGAAGAGGGGAGCGGCCTCTATCAGGAGTCTTCACAACAAAGGAATGCATTCTCACACTCGAGGGATACCGTTCAGGGACTAGAAAGGGAGAGAAGATTAGTTGAATAGGGAGCCTCCTCGTTGGAGACAACACAAATTCGTAAGTTCGCGACCACGAGAGACGAAGTTATACCTTTCCATACTCCTCGGGAGAGGCGTTTCCCAAGGAGCGAGGGGAGAACCTTCGTTCTTTCCGACATGGAAGAAGGGAAGATTCAAGCACCTTCAGAAGAGAGGCAGGGTATTCCGAGCCGCCACCTCCACTAGATGCTATACACCTTCAGAGTTAGATACTCGCACGAGGAAAGCTTGAGGATATGAATATCATTGAAGAGGATAGTTGACACAGAGGGACAAGGATATATACTCATACTCACATTCGTGATTAATATATAAGCACGAGTATTATCAGTTACCAAAAAGAGGGGGAGAAGCAGTATGGCCCCGTAGAATTATGCGTATACTACTAACAATACACTCGTATAGGGAGCATCATTATTTGCGCGGAGGAGACGCATCAAACATTCAGTCACGGAGAGAGAGCAACGATAACTTCATATATATAATAGAAATATAATAATATCTCTACACATTACTTTGTGTGAGTACGTCTTCTCTCCCGAAGGTATAGAGTAAGTAGAGACCATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 21:58:50 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +PVDATA +PV;cDATA +PVDATA +PVDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~$LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS?KP1AMbP1RL[P1WDiP2AMwP2BA~P2RLpPBASCPBAS?~PCONX PCONTPDMFPDMF?bPLOCMPLOCFPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT :RUNT 3RUNT 97Rate RunN""S/N%?CSCAN3SMED SMLtSMPL SPACAXsESPAC?[SPACAXsESVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCH?yphDYbigphQLcphTRphTRdThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +PVDATA +PV;cDATA +PVDATA +PVDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~$LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS?KP1AMbP1RL[P1WDiP2AMwP2BA~P2RLpPBASCPBAS?~PCONX PCONTPDMFPDMF?bPLOCMPLOCFPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT :RUNT 3RUNT 97Rate RunN""S/N%?CSCAN3SMED SMLtSMPL SPACAXsESPAC?[SPACAXsESVER4.0SVER SVER,,Scal@Scan3TUBEB4Tmpr<UserphARphCH?yphDYbigphQLcphTRphTR,9drWdGY| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz99#memIzpY?RV"}d!-V\,5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! 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