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" # &  " "&                #    +&*  $!"4%'" 4"! *# )$ !)#%0!'  % &      '    !  $                     (,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e500728541034c93891c1298f0e12e96Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_H8KB_3730_POP7_BDTv3.mobSeqPop7z ?4jSE ff<=n>>$DX$Und9zJiPp>)h6e|sO%i(M^ZJ4peaciuqLARu@o?S[ QxxeWQOI6WiA" qo%*,./& Pl9fRJFA7( +/,*2Kv:R`jtnSfOZb7Uf""U\5 -9%]xM#?ff<kG' ',$!Pt]ST]isxuhS:! 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J U ` i v    ( / ; F X d p y  !08AKV_kx'0?MYcoz +5=IR\hqy '2AO[dlw *5BL[env (7FRZcpx %7CJV^js|  3=FU_ny !.5FUdow&8BR_gs}%2@KU&3>IU_es ,5>I[iu(7AL[iz ".@MWcq$-:MXj%0<G\jx *:GSds~ "-7BO[o{%5@Rdo  ! * 5 A S ` h w   " 0 : E W b m y   " - 7 D U c m y   ) 4 ? J U ` i v    ( / ; F X d p y  !08AKV_kx'0?MYcoz +5=IR\hqy '2AO[dlw *5BL[env (7FRZcpx %7CJV^js|  3=FU_ny !.5FUdow&8BR_gs}%2@KU           &                                                                                              '$                         &                                                                                              '$              U XS9QGQLcEI LPAIL1Y5OKI!&67&>,C,+@ZF,5Z`H!X"=_BSKK#"+LCR>\)]DE;$VA9#>TT=]K;Z[ X 0MZa9 .%='d=G"-= 3R>#R;H4]Y G ;Q 3#AHI72A45D$?)DSX0N_N43+!EM5S8<;M$/aO`V3?]SD TAU5C/Yc5c [O8c&OK$$!"3/LD9@!SZ9<R; TI(WW#(9O:_ *^aTRUT*UK"aKFb`)L9T+F.` P(-=<T b4LGbXA ITK_ 8]B X2(K d<0*\/YF6I5[UU>I1 JPV 6B [aBB7S O )(Z5/a-E -%L:95C;$>PX8A02?EVG? B-: @#;$8QY` 8$M> TR0%6/L*bE+/5W.DI 3EB04MWII 0/N"I(W0V8\ OYC(2/DXNCE1KL'6?.P ,XOZScG-s\3`9"1</,~vGNwwqsBcW. COfIrX KuD@ #xX C%IRCweu*bD>j+$ORnqw$VR:$ Xoq7w Pfk0XEb5S^>Xk$?Lyap{`B0eO"/F$;)r)_w 2EmEbu <+>0 (^ V$G shAHiu XYjqtDd)GX-88#,5$5l- e h_v6 b>C/4? 4 ]%D QLB%tEd}@M@^} ]z Em-h^HSor09aIBgdj  yFBEj Em9@3]@<Hza<6e^ZK;\`t8l*PEF;^j[:K8%\RY&w0I) B.$  :JB@ 1% Q6=lh*;V)&#> Q$,:_:7y+6 W0=EG2=(Q1 >Ap(%7pb;ibp'lcCkyye {Sq@pCoWp-WK/RpTXPq )8Av3@l7K;:* jw',&qxVp_I]tU7h*&Wh7{qaw~+~LxA4e +9]$8mbddd[T% vZzryTo z!0=[`K,Nqxwdgt}i8LHmf5Rm%RI8kx0/}Q"uPs e`}J; 66u98!@psH(kzjE*; d3p,\Iu%S&,.QA9r~^LFjvDBMVES% 61N99eo.eZZ^+mM z RyM"A6#{{K61!g;9[tu8DI\{(bMb m3,FaA(]/[*:wA`!? FUNWRKs H Nd lUfMp4U4[:t-%Ko*r!3YbYM :}?.m5'EmmBa3T9zL i+[|:!lz'4kf:~AkoR:.A+i/  Dzj:KK?QLW\P3"}5 Zf[ W@}r,nK*(*RWa({c,KC$ggK\LR;pM&<&;s4EWu13GAAGCCCCCTACCCCCGGGATCGGAATCCTTCTCCCTCAAATTGAGGACCCCTCCTACGAGACTGGAACCCGTGTACCATATCACAAGTTATCTAATAAAAGGGCTTAGGGAATAAAGTCACCGTGTGTGTTTACCCGATTTGGAGTGGGGCCATCTTATACTTTATTCCGGAAAATTATCAAATTACTAAATTCCGGCAATATCGGTTAGGGTTATCAACTTCAAGCGCAGGGTCCTAAAAGACTTGGTTGGTGCAATGCATCTGTAACTTGTGGCGATGATGCCCAACTTCCCCGATAGTAGAAAGGTTGAGGGTCTCCCACGTCGCTCAACAAATGGGTACGGCTTTCGTAACAAGACAACCTGACCGGCCACAACGTATATCACTTAACTCCGTTCCCTACGACAGCAAGTGCGACTATTCCATTACTCGAAATCTCCTTCAGCTTTTGAATGCCCTCAGCAAGATGAATCAATGCGAGCGTGTAAATCGAATGCTCTCCCCCTCCAATAGTTGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:03 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS(ZBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #oFDATA #oF+wDATA #oFrUDATA #oF3DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE2LAST~~&LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMP1RLP1WDP2AM P2BA$P2RLPBASSPBASLPCON}PCON vPDMFyPDMF0PLOChPLOCZPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPAC)SPACMSVER4.0SVER (OSVER,,nScal@Scan3TUBEH8Tmpr<UserphARphCHGphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS(ZBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #oFDATA #oF+wDATA #oFrUDATA #oF3DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab6FVoc6FWO_GATCFeat..6uGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE2LAST~~&LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMP1RLP1WDP2AM P2BA$P2RLPBASSPBASLPCON}PCON vPDMFyPDMF0PLOChPLOCZPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPAC)SPACMSVER4.0SVER (OSVER,,nScal@Scan3TUBEH8Tmpr<UserphARphCHGphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i8Fb$tM%+5b=LZ=cbEZfoB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZWT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS:Q&:C!g61CfFc':Z^mDt4)Mz99#memIzpY+R."}5u-H+U5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,|`Dp>H,o}Qm4'p=R .*}Lq{F}u^zq+pBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si!AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``DB1PtB1PtBCTS(ZBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #oFDATA #oF+wDATA #oFrUDATA #oF3DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab6FVoc6FWO_GATCFeat..6uGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE2LAST~~&LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AMP1RLP1WDP2AM P2BA$P2RLPBASSPBASLPCON}PCON vPDMFyPDMF0PLOChPLOCZPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%SCAN3SMED SMLtSMPL pSPACMSPAC)SPACMSVER4.0SVER (OSVER,,nScal@Scan3TUBEH8Tmpr<UserphARphCHGphDYbigphQLcphTRphTR