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'    $             (%, !%                       -*%'#"$'(89)5*$*!',#        !$$                # "             & !      #!+    !         + + %# +   '"'55S<H5hZh[STThNLMXE-=#*     (,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 84109751bda74cf8a2d28929ddd86668Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_H6KB_3730_POP7_BDTv3.mobSeqPop7z]aqjqM1$!$!|{@nG>TsWC3 d2(u xWY&  zA>| g T  ^  ~ o   w - |  u )gSOg0rE)#0CNI6 DTP)y-f 8  8  V  Q   % ; 5  : V E ; " |B{u]#_  {M%wO-Q}t(@Rf)NqX**7D]$oC" -0$ Wy+rd`erytyoR1 +56.!,=GG@9:FYksjR4 '.-%!Eh~lL*+In P;8}R20Xz> pN.4SjpcF$ (693(  -9:1! 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'9DE<--B]xoTA:=CD;* *<HI<&#//$:\v|lL(   $-/(/FRM7&MjtgJ'        $A]r}oO3 ,COK9 &&"'&  3?>2 4Rl{|rbQE@AFKLH=. );B=0$",>NTK6    2><+7Q[Q8 3QshK1 #;LPF1"7EG<)   %161# !#(*' !).,"#4<7)'4:90$  !%"   +8Q9@ڏ\AnAB&A4nKB.bcpKB_3730_POP7_BDTv3.mobtermAAACAAAAGGAGCTTCTGGGTGAAAGAAGGTCTTTTTTGGTTTATTACGGGCTTTCCCCCCCAAGTAGAAGTGCGCTTAACTTATTCCTCCAAAAAACCCGTTCCCCTTTTTTACAAAAGGGTGGGAGTATTCTTGGTTTATATATTTCTCTTGGGTGAAAGAAGAGACCTTTTTTTCTTTTTCCCACCACCCACCCCGATTAATAAAAAACTCTCGGGGGGGCTTTTAAGAAAAAATTTTTTTTTGGAAGTAAAAAAAATGGGAAAAATCTGCTGTATGAATTGGGCGGTGCCAAACCTTTGGTGGGTCAAGGACCCTTTCATGGGTTTATTAGAAAAAAAGAAAATCCCTTCTGGAAGAAAAGGCTACCTGACTTAACATGAAGAGAAGTAACGCTACTCGTGCGTATGGTTATAAACAAAAGGAGCTTCTGGGTGAAAGAAGGTCTTTTTTGGTTTATTACGGGCTTTCCCCCCCAAGTAGAAGTGCGCTTAACTTATTCCTCCAAAAAACCCGTTCCCCTTTTTTACAAAAGGGTGGGAGTATTCTTGGTTTATATATTTCTCTTGGGTGAAAGAAGAGACCTTTTTTTCTTTTTCCCACCACCCACCCCGATTAATAAAAAACTCTCGGGGGGGCTTTTAAGAAAAAATTTTTTTTTGGAAGTAAAAAAAATGGGAAAAATCTGCTGTATGAATTGGGCGGTGCCAAACCTTTGGTGGGTCAAGGACCCTTTCATGGGTTTATTAGAAAAAAAGAAAATCCCTTCTGGAAGAAAAGGCTACCTGACTTAACATGAAGAGAAGTAACGCTACTCGTGCGTATGGTTAT(6>IT]dov&4=IUdt "+:DOan{ $0=GWdu$3>IRal '3@IWdq| ,9GR^ju "/<IUakw!.;KXes   # 0 : J ^ i u    , ? 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K 7T5^&/I(-?^3@TO<T%^^A.SC?E1:DVd7^Z!02@"P_)@UXR$a3^cM* CX=.TZ>G'4593R'4N'YDI D-A:%"!$B 87BW*\XJ a?+UWQaYS5'!61A+15N4+<bb* N,X`%CRY*=[-E6X]\"D1_-C`R/G&R'=`+cd=[& D&+5(M=SUWWS]U@04PBb* 5TCY".T0Y?\(RC2 SF< # !95/C2AOX/U]C?VH), %Q W]F[,W_cXKZ88 LUaE?,E4\C3C]CX&D=\D<D<7WDD40g s 0 jP1. 7f` WQwfBKFfIaHB4c\X[q$A9fC]*M@:}3N9v1&BK+Y;$B]bHu}#z h: +\WRLh:YBNF$-k.>H 6D/ oR>#!zX9J L9)W e>P f)0?0D>_=WZ_`)q^q>qt":+2gL;g\D;VAN}}.\: D2]0l tW'<c+'+ l~iJ256 ~ (us wc3YO+|GqAA&uP9^e? $D %n<hICYk <G9>`{#4KZ(iP>X#Nw  d,c# M\2Z%T \OV VFL(V6)y 9C/0 FaB2#<N c7Z"9  :  m('6k] :BVI _ HdX  3tNB@;BCRY i9WieUJ\/BdodIIu ,j2Wl[RduZYoS[a>L>Kp4Moc&>NPy,sdPrmJ#-r/GrefH, _Yd>.Yk-UNsW`h 0'd:*KwlMl0>% :B}3A&aea8o;{+"8j oOkUcV, 5QH3v~ v d HE=x{Rj yN:+^IS{nJ75`##]+j&/# 2/,If~WbDSS//)\s[ O`K}?G R:3wz1)bjb?s[ygp{oO$)*Ff"]SwX :VJfHx2aqw4M# Z-\cB^,Ex_ Eu T7XpmdA;xW5;UrC+ C$HMBwkTZ b0r \pTMGGGACCCCCCGAGAATATTAATGTGAGGTCCTCCCCCATCCAATAACAAATTCCCTTTTTAGGGAATTGGAGTGCGAAGGACCCACAACTACCGGGGGTGTGATTTTCCCCGCCACCGGAAAACAAGAGCGATCCTTCCCTGTATAAATCTCCTTTGTGGGAGGAGACTACCCCCCGTGCGCCTTTTGACAAACATTTCTAATTCCGGCCCACTCTTTAATATTCCCGTTAGGGGGCAAGACCACGAAGTAACTTGGGCCACCCCCGGTCTCTGCAAGGTTTCGCATGTCCAAACGGTGGAAAAATATAACCCCCTTTCCCTTCCAAGAGTACCCGGTCCCGACCTTCTTTGGTCCACGTTCGTACGGCTTCCTAAATTGGAATGAGAGTAATGAAAGCGCTTCCGTATATTCCTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:27:48 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSc=BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA +WDATA +W<DATA +WDATA +WDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~aLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISBP1AM@SP1RL@OP1WD@VTP2AM@\P2BA`P2RL@YPBASDtPBASBPCONN4PCONLPDMFyPDMFBPLOC@ITPLOC@FPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%BSCAN3SMED SMLtSMPL pSPACMSPACBSPACMSVER4.0SVER c2SVER,,nScal@Scan3TUBEH6Tmpr<UserphARphCHBphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSc=BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA +WDATA +W<DATA +WDATA +WDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabqFVocqFWO_GATCFeat..qXGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~aLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISBP1AM@SP1RL@OP1WD@VTP2AM@\P2BA`P2RL@YPBASDtPBASBPCONN4PCONLPDMFyPDMFBPLOC@ITPLOC@FPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%BSCAN3SMED SMLtSMPL pSPACMSPACBSPACMSVER4.0SVER c2SVER,,nScal@Scan3TUBEH6Tmpr<UserphARphCHBphDYbigphQLcphTRphTRa,pEnO"#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? VCCHY<6(mu y-WLa)&:\.~x|MI9?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3WAd@EaZL)E^rkrOW9;(dC18{ZWFrbT;fQGKekx&#v3"qo,D`&Tj1t9z"DEoh90S*o_cM&+#u?GG5)ljD_P?Yf]4'Uw\Pcc{\A'ZvXTP>I_n@(Zf.kRr+4#3y4E^h9r4uoak"T'o7LA;mq;;9VgO(_y"`k:!.tkSY4E2 (#>+6,j"bpVOygqGX*_V&S$~=}F)h>;-T)RJTZmU)P? sIe]7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]wclQ[>A{%i/"E.Om,\eyDW"[Fw.eV$((jHm]#.J0jy1F5l]hl5cS_d.u?zAw3+fF<7:cFU.F4/~L][U_1(_CcS{[}7"=I).zP{?7.N{Cj_"@?j~J!Eb4;,}E -kb+N4+t457fc|+{Wj2oFtX>j3XV]'p8NpdNmbcn3?GY\U {%KTfx'H9Kx'!byXYhRwZj1& evr.FZH~.cW;Tcb?A(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"@>oW~!.eHB,}&s-@M+9mW'>YP~jM.9Wl[Z,9AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``B1PtB1PtBCTSc=BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA +WDATA +W<DATA +WDATA +WDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabqFVocqFWO_GATCFeat..qXGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~aLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISBP1AM@SP1RL@OP1WD@VTP2AM@\P2BA`P2RL@YPBASDtPBASBPCONN4PCONLPDMFyPDMFBPLOC@ITPLOC@FPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%BSCAN3SMED SMLtSMPL pSPACMSPACBSPACMSVER4.0SVER c2SVER,,nScal@Scan3TUBEH6Tmpr<UserphARphCHBphDYbigphQLcphTRphTR