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(,+/A%$&" #4   6"+,@),4,+9!(#0##)"/XKgeqpf'G;&.;;N1.;<fj@!(  #  ,1'!%*4(#$0( # &6,DTp}f/BS"A.bH@'!      / $93$.5RVS{/,,B=A[E@$     ! 1F@_yAya)H.dZCz.`hVA:"7  ('(( ( 6%&JQe^d8 n4@lVu!ukK9      ,     $#  ',/6*:/.',&5'--</#**HA;Rao.9-U.-|5sWL86-)#$*;$&),!! 5,E5F| h&-q>Y. i0foW'2v+||1&|2@? _Y2<#    "QPw%ng*!Jbw0qe4-, $ " //CeoRF|`,3X44    -%-5%"" + &09599IQjlT\>8a%T<c%xH$\/bE!8{2$ Cd1Xv(Ok_tzNm80GxhUbF,6hyUHO=??B*G;)3UCNdkI| IRdvh<1x8la?18 "8M4ksl~P[((,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4a8091be7a10439d98a611156036d26dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_H3KB_3730_POP7_BDTv3.mobSeqPop7z((''&'(*-./..-.03688753236;@DEDA=97789;;9630..0258:;;9730-+)(()+.14541-*),18?EINU^jx/?MZcggc\UMC8(sg\TNJFC@=<<;;:86569<>@?=;:;=@BBA@?>?@AAAAABEJR]l%bS' uTt~rS%`@    .Ott=]g!iQ@4,(%$%&(*,--,+**+-/023456777:?JYmNUdJ8-'%$#""'3LubVooq1&|I(         'N5 sw>       Pb&_kFX! 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'HJF5ZRb!C= #,c[G8!#`\%0Z G/% bERYBd#ZW&)9[:`5Y;RDR \-G SIV\TBH&!6CR VL+KO3 V5OI\UR);T_'CDO@%(.<_$'K8]A( 2^)D8*3Q/d44.&SS@+!/T,<K':;9? ,O)A\?H)Z0'V^\$Y)<+T3@A > .%_G <A  7 :1] 8\Q-+9)3X"D?G+_SHUG#/OB/JXB2V<aYZN+WPAN USS5[Q(#DLZB U #5)>E>NHZF\'S%N=Y #&ER8;QN&(B0+Hc,`DN=7U+I[#7#"01.-`W&EOGI5,cGQM^-8 `7Z%HQSX-IU+6<YI/%"TM4U.\]?U O [\F((6QL[<9- 'GY*O+P6)?:%4<=C9\).TM! =I 0b<]c[aF+*_`2_SCc&,\Y"]a?&IS>$*])[0$OY'"-J, $:b YL8)>cKQCN;0S\b(0451C\<>RZ.MH' a[a2(O GF )U8'RS(B.`/,#!$BD1]J:(aOL^X(C S)6G B;; .%]O FYU)\UH["UaO5b!S+-6 [[aTQ# PKK;*>-^^^&RXK@ZKIC2 !03I!?*@ >a D&I ; 2[**&I19MY<E4A%V2N-#<V %2XY[c#R5iJVZ7D)Dg\ih~P7zN@pRD8@e^:Rvc "T .9j_6BTlH=jWa| !Ti>@#XA0Fy^\eT82j>WmW=@?E55dr -;D*`m47uB(",4CDGJBGQF6AeZo"(NZy"u-XhDoRs{GHOi%<>snus!i[Ht}&oAoS|;:p v$a/DZNR[v%Ka&U!rVluCKZnMNA'CJG(4ie[buP0Jl!?faB 1GF;ThsW>6\1@fK3( -<E>V{ J>{<;nKR"jJCD-3>Z u!k26iays6"Wm6qhgxW[Iby" )7}1`K> zpBwz AQ Iy_G0EQ[\l1]r [JY)c4 OY<-+_K}WR ZKM!l $X  HPi'  =,{phRMC`6)cJEU/8 -0.6+84.$(  xxxxx                 ! &  "   ("+%(' 4$" $*&/1'!>'3& )8,1'- *.:A*1D08)37+8/&HbAAAA663A08(DMN=,:LD=(E#0mC0=@>TD7YJE7CHLQiii\\HYRumb`KNZJTpEQ[RaVLX`VUgPRYZloctfhdxhb||||fehgib|`wq}kn|ou|{/ a]p!ps.^X% 4dSp0f;}VMp. cVQnTd^"XOdUKy)C*mdpc-K6jy[ 5N?pnd5#b NT]WQOc eUJN?U trN>wpM\v8zY!#$ !$.("%0/$  $##%R$$%3,/#0*4 % +"5!11$ S(054";@;#1JC+M+C+;;0%N680'/) F;:q]P%4=/B5~32s<HYF.!XL/$08ME&-"0^k-: ;N_.M;?PNbaHNj@W`JS9d4cu:fyqqa2ycGL[Y&bb[R<ajgk N{S]ZnQSP!lkHOY"O&rj"5 ~IjD>+#S)\rsP`nX: )MB, CTAAACTCGATAGTATATGATACGTCTCCTCCACACACATAATAAGACTCCCTCGACAAGTATAGAAGGACCCTTATAAACCTACCTCCACCGAGGCGTTCTGCTACTCAAACACCTGGGGGGAACGGAGGCTCCTCCTCCTCATATATGACTCAGGAAGACAAGGACACGTCTTCTCCCTCCTCCCTCCTCACGCACTATCCTCTTAAATAATATCCATCTCGAGTAGAGTCCCTCGAGGAAGTATATCCCTCTAAAGTGGTATAGGGTCTCGTAGAGGTCTCAGTTATGATTACACTCCTCACTTTCTGTTGATCCTCGAAGCTTCCGTGGTTTAATGTGAGAAAGAAGGAATTTTCTCTCCTCAATCGACGGGAAAGTTGCCTCGAAAGAAGGATGGGAAGGTAGCACTTCGTGACTCAAGGAATTTGTCTTACCCCTATAAACTCCCACACGGAAGGAATATTCATATGCAACTCATCTACGGTTCCTTTAAAGTAAATAAAATATATGCAACGAGACAGACTATCTATCAAACGGATCTGAGGACACGGATCAGAGTTTACATACCTATGAACTATTATACTAATGAGGAGCTAGACATTCATACTACGAATAATTCAGTTAGAATAACAAGATATGGATCACTAAAGTACATCATAATAACGTATTACAATAATGCAGCTGGTTCTTATAATAACATACTCATATAATAGACAGCTAGGTCCGCATATACACAGTTCTCAAACTCAATAATATAGCATATCCTACGGGGGGGACACAATATATAGTATGGAATATACCCTAAACCAACTCGGTAAAAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:27:32 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSqpBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SvDATA )Sv8DATA )SvDATA )SvDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~oLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS2yP1AM9rRP1RL9rLtP1WD9rYXP2AM9rf<P2BA99lP2RL9r_PBAS995PBAS992PCON99I;PCON99FPDMFyPDMF2PLOC9r?PLOC9r9PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%2qSCAN3SMED SMLtSMPL pSPACA\^SPAC2SPACA\^SVER4.0SVER qeSVER,,nScal@Scan3TUBEH3Tmpr<UserphARphCH2phDYbigphQLcphTRphTR"This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSqpBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SvDATA )Sv8DATA )SvDATA )SvDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~oLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS2yP1AM9rRP1RL9rLtP1WD9rYXP2AM9rf<P2BA99lP2RL9r_PBAS995PBAS992PCON99I;PCON99FPDMFyPDMF2PLOC9r?PLOC9r9PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%2qSCAN3SMED SMLtSMPL pSPACA\^SPAC2SPACA\^SVER4.0SVER qeSVER,,nScal@Scan3TUBEH3Tmpr<UserphARphCH2phDYbigphQLcphTRphTR,*^(`P. O~JcW`@0'q qoVU-9k *c@{UaG6EG>dcnxgJB7`DK,*Tijh.iOW/D-]$/pTFV2TVyLyD,kSagv9'i?oX$^io4)X^UM]|ny!.qK|C!^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| Vkyc p7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/vO3G`8hC0rOHJJr9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YzXr$cn?:;wWG{uHYs+/e_'UvBVvwp#_T99Wq3F:{SocJy>@ YlGcRb[xy8J9tG>udrV=y`6@TmkcDynWa9?QLwUF3TP.&"D|;f[5kr+E{@2K}d1)I+=l~+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~beaA({]/wQ2a52rW.X}=1m)h>VO@A$^XcHjR#7JhWVE4MP#hO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFivFUUsDG+TK#6] TRFM/|gEmpt^14t.N^n"AQcG[ncb V+YbqsC(Y5_}4]'o>s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52rRU)>`V$];9'k0K3AQZ`K'H:{GKipM`{VL7f4AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``B1PtB1PtBCTSqpBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SvDATA )Sv8DATA )SvDATA )SvDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~oLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS2yP1AM9rRP1RL9rLtP1WD9rYXP2AM9rf<P2BA99lP2RL9r_PBAS995PBAS992PCON99I;PCON99FPDMFyPDMF2PLOC9r?PLOC9r9PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%2qSCAN3SMED SMLtSMPL pSPACA\^SPAC2SPACA\^SVER4.0SVER qeSVER,,nScal@Scan3TUBEH3Tmpr<UserphARphCH2phDYbigphQLcphTRphTR