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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 72f2d2619bc3469bbd204a19a8769a07Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_H1KB_3730_POP7_BDTv3.mobSeqPop7z#####$$%&'(()*+-...-,,-...-,*('()*+,+*))))*+,.//////....--------,+)(&%&'()))''')+/257;?ELSZaiu*@WnzgR<%tcVNJGEDA?<;9999:;=>@AABBCDDEFGIKNQUX\ahq~,nn<qXk6W ^C6248>DJOSVWY]hyn)/EfRE<74344443323467763/+'%#""$'.7DUk7k PqYI>843349CVwM>VZ ]8Z7       7k0hD[G^y6    5gR$9U)        !&+022/)# '@r(x-<w= (Z&[&     !"! m&7ZY0&$ "%&#&*,+&! 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& %;)#.%  $ '#!#.   ,  (D|<*  % )>%1!'3#(Z(' %M%" $11 ##9,R  -D@2;!+': Fa-";+mS)9,'9Z+D3F.4*9fi--R ?:(7G=T3:,+?R*%+',0D7;>h3_NgLTYY898G6>K'.[9S#&Cf@D3MI6<&%B% L 3^8Cj*TYoZF\Sl^EdoaYQH<~6Tuvlsyc[Hql>+sX UqNtnwi3&r FC eFx67Pt:)+]!a%UugVl8j9'{+j\]t'O   CAACTTCTCGAAGTATATTGATACGTCTCCTCCGCACACGAAATAATACTCCCTCACCGAGTATGGTGGGGGCAGTATTGTGATAACTCGACCGCGTGCTCTTGCTTCTCAAAAACCGAGAGGGAACCGATGATACTCATACTCATATAGGGCTCACGAAGGAAAGTATGTGTCTTTAACCTCCTCCCTCCACACCATCTATCCTCATAAACAACAACGACCTCCTCAAGAGCTCCTCGAGGGCGGGTCTAAATCGGAAGGGGTATTGGGCCTCGTGATCTCCTCCATGATGTTCAGGAAGGACCTCAATTGGTTCGTCTGGAAGTCGAAGCTTCATTTGTTTACTCGAAGAAACAATTGAGGGTCTCCAGGGAATCCTTGGGAAAGTAGTCTCAAAACAAGGATGGAAAGGGGCTCCATGGAGTCTCAAAGTTTTTATTTCTCCCCGCGCCGAAGACACATTCGAGGAATAAATATACGGTTCGGAGGTTCGAATACTTTCGATAGAAGGTACTATATCAAACGAGCCCTAGTAAGCATTCCTTCGTAACACAGAAACGTACTATAGGGTACACAACGAGTAATTATTACTATAATGAGGAGCTATACATTCGTACTACTAATACTTCTGTTGGTGTGTTAGTCGATTGAGAGTTAAAGTATCATATAATACCGTCTTACAATAATGCGGCTGGGTACGCTTAAATATTCTACTAGTATACTCGACAGCTGGGTACGCATATACACAGTTCTCACTCATAATAACAGAGCATATCCTACGCCGGGGAGAGGGTGTATATTAACGTATATATACTGACCCCCCCTCGGTGATAGTTTCCGAATTTGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:27:16 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt ASPt B1PtB1PtBCTShBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (\PDATA (\P5QDATA (\P DATA (\PDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~fLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS'P1AMOHP1RLOB,P1WDOOhP2AMO\P2BAOOcBP2RLOVPBASOO+PBASOO'PCONOO>PCONOO;PDMFyPDMF'PLOCO4PLOCO.RPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%'ySCAN3SMED SMLtSMPL pSPACAaSPAC'SPACAaSVER4.0SVER hSVER,,nScal@Scan3TUBEH1Tmpr<UserphARphCH'phDYbigphQLcphTRphTR,This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt ASPt B1PtB1PtBCTShBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (\PDATA (\P5QDATA (\P DATA (\PDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabvuFVocvdFWO_GATCFeat//v5GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~fLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS'P1AMOHP1RLOB,P1WDOOhP2AMO\P2BAOOcBP2RLOVPBASOO+PBASOO'PCONOO>PCONOO;PDMFyPDMF'PLOCO4PLOCO.RPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%'ySCAN3SMED SMLtSMPL pSPACAaSPAC'SPACAaSVER4.0SVER hSVER,,nScal@Scan3TUBEH1Tmpr<UserphARphCH'phDYbigphQLcphTRphTRT7K4]O'T:cGave>u\nLTH@i=R/4zWvR'#]7&t1&^Ab{?nzF2}THOj-F'\/~X;Frxp/Ygnx'[vA-\K"L1{!Ulb|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+$#t{~FOI*aW$Aj#0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,,v1%$'#ttA4[>wXAr:3%6AB$+A?@*>-PM7M=a*N7lk;)+(H|XY+C}Q*xP!t p7YwV&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBArL-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bfkmbp9Y A.]}'UyvH@duU4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnPk=o?eUKGM5J_bi(l{~Gj`X0?888%g^^7FDg;AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt ASPt AUDT``B1PtB1PtBCTShBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (\PDATA (\P5QDATA (\P DATA (\PDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabvuFVocvdFWO_GATCFeat//v5GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~fLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS'P1AMOHP1RLOB,P1WDOOhP2AMO\P2BAOOcBP2RLOVPBASOO+PBASOO'PCONOO>PCONOO;PDMFyPDMF'PLOCO4PLOCO.RPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%'ySCAN3SMED SMLtSMPL pSPACAaSPAC'SPACAaSVER4.0SVER hSVER,,nScal@Scan3TUBEH1Tmpr<UserphARphCH'phDYbigphQLcphTRphTR