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"    !    )#  &'    0  #              !!)+  !$%4%&+,'(('&-,!&&&,-+((222@CDEC<94.**"* *  !" !     !#  "$$   (&/>7:@YU`n[dMLM9*;3."2 ! !60%/$FTKE=A@KMEBIL@IFB3>(                                 #(!./'FSlr|yyjpaioxk]IK@N8%                            % , <?F`\{ @6I>5=&%$+{_`VE<99<6$'?:1:'&/#" (,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: daa2d86dedf1433cb8cb679b8d6802e2Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_F8KB_3730_POP7_BDTv3.mobSeqPop7z4=5$ %L|AB~f# yy "/3- *7979If-gY.ya_[ U~-m&!=(P&DJ:#AyO "  z U  | ){.g;M\*'8=2Gv.75*b;%%9VnwmU5lbUA# ,u`0yP9?^*n>6IM !cuenDs}gQ;&k?k(T/)AlU' %:ED:13Eevid`WG02J\ejozg@ V+[x~mV>2;_36F{jvl6*?Q`kojYA%(?VisqeR<*#+Be^:%%6L\\L/ :vxN=GfhTRd=kz1 .-"zt5hd8pSC>>?;3( 8Yzb@! <^{sfZN@.     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R(9=$=_TLU7<N*A+53cC2.;U$'HU-I^ / );_9+A&<M/B cGR545 %KS  N.M5SH<V* ".>E1;G%#6#3PB* *]F 4]![NHc?Ad9:;7 9&`V*90 OO,#9@@*)#KQD*'-%QA@O)(F%c%D+\H0)ESX>87450;@C 'EI8)N(+Kc"EA+6cOB<? ; [:8IE/\ PP0 E8Fb#T <\D=<9:TV0SN1T*B F')( 9<1<'>^!CX! ] @"QF EF<?I 28P2 :<`R-8EE% ##*Z 5B' L34dOA8J -,S11KM36=>!Y@5PB ]1Ybd ;B Y`IJ]A$/IITXc7  /9+%NH\:TB)T>@=<aq R^; zb :Zdy#^j  m#8W?Y ]%pXh P /G1pl!t(T_Xa8~r[(\baBR<oRG3. >uK<a@p.0~W9([Ypv!)DA#{C{.hMcjgF lqJI<jy)\rX%@]c[p;%S1t! )1[PorZwIUNiF8&8Wj3hk!X:z]q7\0(_R1$EW$(H* U vvB^EtVpjn'  & P~D =mA|C6X0f l {Dg(/;y74NX  60v<Dt '0*[3 >( (M$ |;+$R  ( - R1 ,1Q " - */ D 4$J {,H*JZAu(m,,ec^Q]E=iSh5>8_&<:1~nu7xZq,KT,< ~GKX^ds/G 'O{,A&+5 ,a2m<{P8 $'[qGsk!\_0C"E?~C( ~DCksW79t &:~ rpw}xDN?z7~d.w% Y;gbH\to8e{*h,<z(1}5j7P}98`Lp?dEpg _mn.J@y?C*A^_!,12 NTc?-iiHQ?3]Vj"W]"(Me{J3PWi1/}6tIKziAML-NM{ltM^Y4VT<F^ #Qlqn0x%/8MV Z;.R4-g?8L*Ts``v]q\y.00[u* A+//HX 2a "aT R\E82_1LkkX\3]K9j]_*8s1s?]CM~_yDFF2^ATA,8L[!Y_AzB]j7L@AACATTTAGCGCTGTTTGAAGTGGAGAAGCCTTCTTCAGCAAAAATCCGAGTCGCTTCTCACGGGTCGCCTTGTATGATGTCTAACATTAAAAGGGTTTTCCTTCCCCCCCCCCCCGGAAATGTAGCAGCATTTCTTATTCTCCTTAAACTCTTTGTTGGGAGGTCGACGACCTTTGGTTTATGAGACCAATCTTCAAACGTAACCATAACGGGGCCCTCTTGGTCACTGACACCTTAGGAAGCTGTGGCCGTAGCGACATCTCTGTCCTGAATCCCTGCAAAATTGGTATCCCCCCTCTTCCTGTGAAGAACCAGGGGCTACCTCGCCCTAGGCTCCCCTATTAGTAGGCGATGAGGCAACCTTTGCAGAATTGGGATCATATATCCGTCCCCTAGAGAGAAGGGTGATGTTCCGACAAACGATTTCTCTCTTATAGAGACTTCCCCAGCTATCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:07 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS#BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #tFDATA #tF+DATA #tFriDATA #tFQDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~"_LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISAP1AMNP1RLP1WDP2AMP2BA P2RLrPBAS=PBAStPCON PCON *PDMFyPDMFXPLOCPLOCPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%9SCAN3SMED SMLtSMPL pSPACMSPACQSPACMSVER4.0SVER #SVER,,nScal@Scan3TUBEF8Tmpr<UserphARphCHophDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS#BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #tFDATA #tF+DATA #tFriDATA #tFQDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab2BFVoc21FWO_GATCFeat..2GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~"_LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISAP1AMNP1RLP1WDP2AMP2BA P2RLrPBAS=PBAStPCON PCON *PDMFyPDMFXPLOCPLOCPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%9SCAN3SMED SMLtSMPL pSPACMSPACQSPACMSVER4.0SVER #SVER,,nScal@Scan3TUBEF8Tmpr<UserphARphCHophDYbigphQLcphTRphTRK2OI*-V~>yj6m}:)9`[?y[nvbeK]TM7l:2Fj62o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbV_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKM\8}48vc. 7$?Z2o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*M0JNbm|XMrDK"*.GHiJ(/\_'MChMoG;KMJ95nM,aVh6dYHp-Op;ZJLgS]jTj.uFk4W7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYCBO(TM::( R[_iNLaFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72Wex(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9} P,hz9_"#kjFO-U[ds=:I1On(="]dr>4>./|xPYWtIbL4);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/il9$M8`+MTk0WC.%o,n`!3CS]n?KGxDIBG(XX7&ZoMQR,9hLAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``@UB1PtB1PtBCTS#BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #tFDATA #tF+DATA #tFriDATA #tFQDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab2BFVoc21FWO_GATCFeat..2GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~"_LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISAP1AMNP1RLP1WDP2AMP2BA P2RLrPBAS=PBAStPCON PCON *PDMFyPDMFXPLOCPLOCPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%9SCAN3SMED SMLtSMPL pSPACMSPACQSPACMSVER4.0SVER #SVER,,nScal@Scan3TUBEF8Tmpr<UserphARphCHophDYbigphQLcphTRphTR