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(,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 48e1e3c775414219bf61c2690e8558a0Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_F1KB_3730_POP7_BDTv3.mobSeqPop7z;]v4Sw1Nk*U} iI&l8U[6|pdXNF@<97420000/-*'%%&(**)'&%&)-03457:>EMWbo~*D\rqU7weXQNNNNLJGDB@?>====>?@BCDDDDDCCCBCDGLT`o?(Li%Tg>c7T"iSE=;;<=<;:987531//1231.*&$$'*-0136:?EKQV]fs6hk>nVE91+&" $1JqTT:CFCUHe@)          A}Go(~5f,e0         D{`Hr? 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($I6{$((-""  =$.73$##K:!#p="/+2,&1-2-/"AQ*#('&X/.D)86*&'"<2)W.H*J!> $]"tZ@02'Z?&_/!<`sV7H46f7!2)*eU.J,-&../+pDv#3@\$BH5 H'-K^Tv}Y`8<J| G+K(zt,RtBDBD[|r[s5TqXtX[t@o1CloDLxPfvzwiimb|ezk`ss(MYd~KW]+/nlE_.4 ! #H`~0"$mp*?KxJUg > aGGGAGTCTCTCAAGTATATGATACGCCTTCTCCGCACAAATAATGATACTCCCTCAACCAGAGAGACCTGGGCAATACAATCATAACTCCACCGGCACCGAGGGATCTTCCTCCTCAAAAACGGGGAGGTAACACATGCTCCTCCTCCTTCTATACACGACAGGAACGTCAGGATAAGGCTCCATCCTTCTCCATCAGCAGGATCTCTCCTCACTAACAAGAACCACATCCTCAAGAGGGCCGGGAGGAAGTATATAAATCCGAAGGGGTATAGAGCCTCGCTCAGGAGTCAACGACAACTCAGCTCGAGCGGACGTGTTGATAATCCGAGCCTCCCTCGTGGAAGGTGAGACGGGGCGAAGTTTATCTACTCCCGCATCGGGAAAGACGCATCAAGAGAAGGATGGAAAGCGAGCCGGGCCCGACGAAGTGTTTTTATCATAAAAAGGGCCCCAAGGTCGTTCAAGGAATAAATATATGGTGCGGCGGTTCGATCACTTTCCCGAGACGGTATAATATCGCACGAATAATCCGATGGAGTCAACTATAACGCTCTAACGTAATATAGCGGACATAAAAATGATATATGAAGATAATGAGGTGAAACACAAGAGTGCTACTAATACGTCTCTTGGTATAACAGTCTATTTATAGTTACCGTATCACACAGTCACGCATTCTAGTTATGACGCTAGGTACAGCTAATAACATACTCATATAATAGAGAGCGCAGTCGGCATATACACAGGTCTCCTCATAATAACAGAGTATATCCTCGGGGTGGGGGTGCGTGTATCGTATGGTATATACACTAATCCACTCAGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:27:20 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSnBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )hRDATA )hR7iDATA )hR9DATA )hR DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mbLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS/P1AM:tPXP1RL:tIP1WD:tVP2AM:tcP2BA::j(P2RL:t]@PBAS::3NPBAS::0PCON::FPCON::CpPDMFyPDMF/PLOC:t<PLOC:t6PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%/SCAN3SMED SMLtSMPL pSPACA_SPAC/SPACA_SVER4.0SVER nSVER,,nScal@Scan3TUBEF1Tmpr<UserphARphCH0phDYbigphQLcphTRphTR-This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSnBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )hRDATA )hR7iDATA )hR9DATA )hR DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab}FFVoc}5FWO_GATCFeat//}GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mbLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS/P1AM:tPXP1RL:tIP1WD:tVP2AM:tcP2BA::j(P2RL:t]@PBAS::3NPBAS::0PCON::FPCON::CpPDMFyPDMF/PLOC:t<PLOC:t6PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%/SCAN3SMED SMLtSMPL pSPACA_SPAC/SPACA_SVER4.0SVER nSVER,,nScal@Scan3TUBEF1Tmpr<UserphARphCH0phDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?&#`h5 KvfQ.w%WZ;P?BH+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f'9u3f0UbBE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WVsN&rMT`r (wHJk:Y`(LV.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#FmFiAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``YB1PtB1PtBCTSnBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )hRDATA )hR7iDATA )hR9DATA )hR DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab}FFVoc}5FWO_GATCFeat//}GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mbLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS/P1AM:tPXP1RL:tIP1WD:tVP2AM:tcP2BA::j(P2RL:t]@PBAS::3NPBAS::0PCON::FPCON::CpPDMFyPDMF/PLOC:t<PLOC:t6PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%/SCAN3SMED SMLtSMPL pSPACA_SPAC/SPACA_SVER4.0SVER nSVER,,nScal@Scan3TUBEF1Tmpr<UserphARphCH0phDYbigphQLcphTRphTR