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(,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1a825284929741619d4ed61b1cbace04Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-Well K1361_E12KB_3730_POP7_BDTv3.mobSeqPop7z$$$%')+--,+*)*,.13455555677766666789:::98643344431/--./00/,(%#"#%')))))*+,.//.,)'%$%'*.38>CJR[ft/DWiyo\G1th\SJD?<:9999:;;;;;;<<=>>?@@@?>=<;;<<==<<;;<>BGNVao"dL>Ipv\@# Z'V {=hSE<7421.+(&$##$$$$$#""#%'*-/1222246;@GP[i}@s3<8&u> xbPA8326=I]|E2Up6_X!7ZQiB)       ,W|gq{|*U&       7`0}jS0      #&)**)('&%&')+,*% +` XhN' ,a+H-WDyPm.  #&(**(%!!| 4k?@o:(& $()'$ !###! 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($[~%ew a\-M y^R]=la9ls>emMO>dQk[W ql;yl&clLYd{ bTs)CKc0kU&S {|^.U@h&$'o Hn{ ` V8zUy@oXTK$>0@W@+"?@x 1$')'%                       !  $+  2*&)# *,<&,1#**@ #C%"*1+26(9-@6 (>E ,3@>#+ !+..1;@63E7&6K>}LKM:=JK7C=BFlFRZ;?PBi=L>EAWNPJUMZ]YFS\X_iy+XSYbTHZTgThkVdpSalZZ]|x{upvebgpofinyr?xxvwz}{a " `15 ` ` ao `L I`w|5#y[p}pK$S~;8s bdX[{S9pedQ Qv|p1J20""^yS! BJjOp6@W@dC="otS,| \Z(0rhv%mmJw|vRddPu pT{*9pdoE1_dq; @8{% L 8wC8,evBp% "E1x,VoC]8;8s*Ktd 8GdKppnpb-Dp\5p%Ru|}8pCM&m8m,qppcppppqRp.pKp C%9OCH<`X2CN/?d820UYDX 1(% A&%Kcg5()&&2!%N; 42-%"(!7( .*,3%_&!-X&& G;  $#/$%"(/ ,"# +!" &! ( - ! #'  &      #!#&" "" ( % ""  ' &-&-&.   $ 4Qx@5& *  63#9- 2 (  "!#*H"#$$- 0H  L @Y5$%$0O29%!#A2*k081J`5LD%'*;/5"f.5Q>B2!27F->I8FFN'@B1HYB;2>MN;/b"s@y=E{(F.'%sD:aB_fC*<N+8O{L[P+XuhSG9(l%0fN\C_mhO{pZ@LR_{ytR^Ssq`OUw_^oc^:}ve| ]ox,!;xK\2kik!%""T#xdE #> gB]Q- 8y"7x  N!()8 X ACACTTCTCTCGGTTGATGATACGCCTCCTCCGAACAAATAATAAGACTCCCTCACCGAGTATGGTGTGGACAGTACACTCCTAATCGTCTGGACGGCGTCTCCTTCTCAAAAACGGGGAGACGAGGGAGGATGCTCAGACTCAGATAGGGATCGGGAGGAAAAGGATAAGTATTTAACCTCATCAAAAGGCACGATCTCTCCTCATAAACAAGAACGATCTCGAGTAGTGAAACTCGAGGGAGTGTCTAAATAGAAAGGGGGAGAGTAGATCGAGAGGGTGTCAAAGAGGAAAAGGGAGAAAAACAGAAGCATAGGTTGGCAAACGAAGCATCGCTTGTTTAATAGAAGAAATAACGGAGGATACTTAGGGCAGGCTGGGGATACTTGTTTCAAAACAAGGGGGGGCAGGGGCCCCTTCCCCTCTAAAGGTTTTTGTTTTAACCCGCTATGTTGCAACATACGAGGAATAAATAGAGGGAAGTAACATACTATTACCTTCAAGTAAAGGTCCTCTATCACACGAGCGTGAATAAGCCATCAAGAGATACACAGATTCTTACTCGCGGGTACGTAACTACGCACTATTACGATAATGAGGTGCGATACATCAATACTACGAAAACTTCTATTAGCGTGACAGTCGATGATGTGGTAAAGTATCACATAATACGATATTCTAATAATGAGGCTGGGTACTGTTAAATAACATACTCTTATAATAGACAGCTAGGTACGCATATACACAGTTCTCACTCATAATAACAGAGCATATCCTATAGGACGGAGAGCGTGTAGCATATCCTATATATACTGACCCCTCTCGTGAAAGAGGAAGTCCGGATGTAGGTATTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:39 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSmBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6pDATA (QFDATA (QDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEXLAST~~kLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+P1AMVMP1RLVFP1WDVT5P2AMVaP2BAVVh9P2RLVZPBASVV/PBASVV,-PCONVVCPCONVV@1PDMFzPDMF,PLOCV9PLOCV2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%+SCAN3SMED SMLtSMPL pSPACA`6xSPAC, SPACA`6xSVER4.0SVER m SVER,,nScal@Scan3TUBEE12Tmpr<UserphARphCH,(phDYbigphQLcphTRphTR-This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSmBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6pDATA (QFDATA (QDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab{sFVoc{bFWO_GATCFeat//{3GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEXLAST~~kLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+P1AMVMP1RLVFP1WDVT5P2AMVaP2BAVVh9P2RLVZPBASVV/PBASVV,-PCONVVCPCONVV@1PDMFzPDMF,PLOCV9PLOCV2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%+SCAN3SMED SMLtSMPL pSPACA`6xSPAC, SPACA`6xSVER4.0SVER m SVER,,nScal@Scan3TUBEE12Tmpr<UserphARphCH,(phDYbigphQLcphTRphTRmkYbCK)l_pD8Jq,./b _.uKxh6O1w^{l{iED>{?oH^}nZ##A+]WJyKPy]Y !T_*|QK:jin'D5?C7bJeoiQ]%[p3}$r&kUV'4MYkftTI|Z{Z[{%;5]U'Q>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W2]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&2$U^X$^(`])>mJGVGkB*Sig]r;a4xG57'!>&7.{Ml>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rTcfBv~HF^95E3Sk-[qr[&X1EDE?]mnjH[$.}RZ#3|5O-U[d1DFW%j'kT'_C:Or4^7~s?K90lVSFsA bXX<,x'EEwiOfFWcDArh]|Ko5qS+SP7T]Tcr[&$&?_1OPh~jTn/])RpikE:b&b)>`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTbbB1PtB1PtBCTSmBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6pDATA (QFDATA (QDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab{sFVoc{bFWO_GATCFeat//{3GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEXLAST~~kLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+P1AMVMP1RLVFP1WDVT5P2AMVaP2BAVVh9P2RLVZPBASVV/PBASVV,-PCONVVCPCONVV@1PDMFzPDMF,PLOCV9PLOCV2PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%+SCAN3SMED SMLtSMPL pSPACA`6xSPAC, SPACA`6xSVER4.0SVER m SVER,,nScal@Scan3TUBEE12Tmpr<UserphARphCH,(phDYbigphQLcphTRphTR