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(,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4e31a39389324d22bae01086081ac1eeSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_D6KB_3730_POP7_BDTv3.mobSeqPop7z  %)+*& $*.0149AJPQJ?3+).6>B@9.#"'+,+**,16983,$!',01101257873,# #*,*% %('"%))'$"!"#$&*18?BB@?BKYhtxtj^USXaimj_P@4.-/231,& &.5751,**,--*&" ! #+3:;6+(6AEC?<?GTcr%b#T| gg{LE + E < `+ZhMCEG=]C @UM o#*|1u8?BEVJWMOP1NKGB\< 5R.H'\  32$&* g,*!O2{p *#ZlfO:/\2d`'L~"!CDAS(pYGAMqg`y[fs1j>Ej;b  [^l rp   $Bdr}J'*r#K/  ygWKB;61*" (.0/+&!  4NiygVG9-"        %,..,+-19AJT_it}~yust{|dOB>CMV[YQD7,%! #(1<GQXYUL@3&   #)09CNW]\UF5#'C_yyfTC5+$ #(.33/'   '.48971*# (.1.)#!#,:IV[YN>.!$('#  !#$""$" ,Oz {YGESjjP8$ ,@VivzteR@3,,059;<:84.'!+3652/19GXfqw{tdVMKOW_cda[WWZ_ehfa[USTWZZVNE=72/+&      $'('# 2MetvkU;"  !/<CA8*$(& $*+% #&$  %5CMPK?.      %,0/(     !!!!!"     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"&)(#  #%$                   ')$  #')($                                                              |@yA,AGA |KB.bcpKB_3730_POP7_BDTv3.mobtermCAGCTTTACTGCTGCGCACGACGATCGTGATATAATACCCCTGCTTATACATCCTCCCCGAGTATCGAATTGGAAAAATCTTATTAGCCAAAGATCCGTTCCCCTTCTTCTCCAGGAAGCAACGAAATTTCTGTTCCTATCTAATCTTCTTGATGATGATGACGAATCCCTTTTTGTTATTCTCCCTTCGAATCCCTCTTATTTATTTTCAAAGGCTTTTGGAGAGATGCTGTTCGAATTCATTTTTATCGAAAGATCAAAGAAATATTACAAAACCCTCCCTGGCCTTCGGGGTTATGACCCATCCTATCGGAGAACCTTTTCTGCACCTTGTTCGGTATCATGGAGAATGCCAACTGGCTTCTCTGGGACATCCTTTCCGACGTCATATGGGAAAATAACTACATGGCTATCCGGCAATGTCGTTTTTCACCGGTGCCACGACACGTGCAGTCAGCTTTACTGCTGCGCACGACGATCGTGATATAATACCCCTGCTTATACATCCTCCCCGAGTATCGAATTGGAAAAATCTTATTAGCCAAAGATCCGTTCCCCTTCTTCTCCAGGAAGCAACGAAATTTCTGTTCCTATCTAATCTTCTTGATGATGATGACGAATCCCTTTTTGTTATTCTCCCTTCGAATCCCTCTTATTTATTTTCAAAGGCTTTTGGAGAGATGCTGTTCGAATTCATTTTTATCGAAAGATCAAAGAAATATTACAAAACCCTCCCTGGCCTTCGGGGTTATGACCCATCCTATCGGAGAACCTTTTCTGCACCTTGTTCGGTATCATGGAGAATGCCAACTGGCTTCTCTGGGACATCCTTTCCGACGTCATATGGGAAAATAACTACATGGCTATCCGGCAATGTCGTTTTTCACCGGTGCCACGACACGTGCAGT +9Ofz%2F[q ")7P_s%=MZk  .:HV_gs&1=IWiw!,9COYlx .<ER\it'2=HR[lw   " . ; F R _ m v    & 2 @ M Y j t |   # . = H R a l w    & 6 D N [ d p {  " . 8 E S \ h u )7AN]jx %.=IVakt (5@KUcp{!/<EQ^mu)3BN_nz &.=JUbm{  /=ET`ov !.;FU_lz '4?IZet} +9Ofz%2F[q ")7P_s%=MZk  .:HV_gs&1=IWiw!,9COYlx .<ER\it'2=HR[lw   " . ; F R _ m v    & 2 @ M Y j t |   # . = H R a l w    & 6 D N [ d p {  " . 8 E S \ h u )7AN]jx %.=IVakt (5@KUcp{!/<EQ^mu)3BN_nz &.=JUbm{  /=ET`ov !.;FU_lz '4?IZet}   #   !'                     "                                     "  "   !                "                     #   !'                     "                                     "  "   !                "                  T!4MGV2FC5?"N?Y>a<VJ9N!Y:Z3P]!!:HEO*Q\6a 1AW X6=\JD>32`A] )2:U]WYKH%).5/NK &A,;@ _7Q# K.KOYG^AE1 %PQ<N1J9X)83?3%W4.30a;QdP&IMA6]3G+  I@MQ.a2[#KZD\b/aa4?^^:F&c9DE! O^c`2"D'E(>ZOO A&R HO6 ?CM;6NH)/11ZP_H.SN\ KSK/) >N &V BE] 9V@3J3F03J,%d$=.8/_U"5]NC G1LN&FcZ+U+G$L, J B("#F9*J#8JVJ8,!=6Na*?F3B] @W1Q-@ I", /=V"F(,(XQ!,I86BW`DJT I^-F{lj@ G 8oA - :2'd&5&u]M #|gQc; "yQQr HcrcT$j?wm2(qh7<;Azth?q8ku[9PM |^N{j[\v@7O/x5|w T?j;\/$nAINm.{..r >P{s#Rjn${|K]2 /* >mPbp^Pr ,<X:v}(m2<.2qd;6m=N]*YF9Mk*% C_Z+8<q   M ` kuju*>lpF{wiZ =5(%1sYD#iz,+'<;wI[x wdAB"5L2ac4R"5}};O*4A[(Fkc6&+7#Q.?6$% T OR i[ G AA' NA ,U# P,I$ X :$1> (2 x/I.,'Y]LWENN5.#VI'x!;?GB U92Q!pW({ {p+#PC&a\">T~3lsM$zB,pd\AM!xNB0!8T. \2KVRUGdv}?&ni*%-[Ry. Q ] zCGL8;5;,  H{5#}AGTTGAAGGATTGAAAAGATTGTTGGAGAGATAGTATAATTGCGAATACAGGGTCTTTACGAGCGTATTCCCCTTTTGAAGCCCCCCAACCCAGAATTGATTTTAATCCTCTTTAAGGAACTACCGCCGCTCCGAGTCTCTATGGGACTCGTTATGGTGGCCAATTGGAGAAACCTGCTCGTATTTCCTTCGAAAACTCCCCCATCAACATCCATGCCCATCGCCCCCCTCTGAGAGGACTCCGAACTCGTGTGAGAACCTTGTCAGGAGTCCCTATTCGTAACATAACATTTTCCCCCCATGGGGTGTATACGCTGTGCCCCGGCTCTTAACGGAAACCCGTCCCGATGAAAATTAACTTCCTATATTTCAGAAACACTTCTTTAGTAGAATACCCCCCTACCTTCCCTACCGTTATTTCTATTGGCACTTTTATGATTCTCCTCGAAAGCAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:27:55 -04:00AEPt3AEPt3APFN APXV2APrN ¿APrV1APrX}}ARTNASPFASPt ASPt B1Pt B1Pt BCTSiBufT"DܔCTIDcCTNMiCTOw܄CTTL ŒCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"D DATA"DNDATA"DDATA ,X(DATA ,X(=)DATA ,X(QDATA ,X(yDCHTDSamDySN mDye#DyeNxDyeN}DyeN‚DyeN‡DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~gLIMS!!•LNTD2LsrPaMCHNMODF ¶MODL3730NAVGNLNE`NOISEOfSc 4P1AMWP1RLT P1WD[$P2AMb<P2BAeP2RL^PBASGPBASEPCONRFPCONPPDMFPDMFEPLOCLPLOCIhPSZE`PTYP܌PXLBRGNm[RMXV1RMdN ERMdV1RMdXNRPrN)RPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate @RunN""9S/N%ESCAN3SMED SMLtSMPL SPACAZ'SPACESPACAZ'SVER4.0SVER i SVER,,Satd 4^Scal@Scan3TUBED6Tmpr<UserphARphCHEphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ¿APrV1APrX}}ARTNASPFASPt ASPt B1Pt B1Pt BCTSiBufT"DܔCTIDcCTNMiCTOw܄CTTL ŒCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"D DATA"DNDATA"DDATA ,X(DATA ,X(=)DATA ,X(QDATA ,X(yDCHTDSamDySN mDye#DyeNxDyeN}DyeN‚DyeN‡DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTabwqFVocw`FWO_GATCFeat..w2GTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~gLIMS!!•LNTD2LsrPaMCHNMODF ¶MODL3730NAVGNLNE`NOISEOfSc 4P1AMWP1RLT P1WD[$P2AMb<P2BAeP2RL^PBASGPBASEPCONRFPCONPPDMFPDMFEPLOCLPLOCIhPSZE`PTYP܌PXLBRGNm[RMXV1RMdN ERMdV1RMdXNRPrN)RPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate @RunN""9S/N%ESCAN3SMED SMLtSMPL SPACAZ'SPACESPACAZ'SVER4.0SVER i SVER,,Satd 4^Scal@Scan3TUBED6Tmpr<UserphARphCHEphDYbigphQLcphTRphTRn(_T+D(_7&OMb'~6/lD-EkOptpuD:\E+mH_a2TS7}_'A\3[}k@_aA+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\dxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]4UvI{OF?jp,V0Id4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oX$^(`])>}JcVGkB*Sig]r;a4g0_[YBPxrl@;Gc+[b`[.b=jrOCy~R^bfFc':Z^xDFU)T;w>xP=O_l;mqy=Y`ge#cme/^it>QG/P'6VMJlWLv'Pn@ )O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#t[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}nz:z5>vQSdW79ZYdj8WoQK5F1_;T,V2%4oNYkk?[C23X}1Fo8/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@