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(,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5d75b299966b444ebc623aadae939cbaSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_C9KB_3730_POP7_BDTv3.mobSeqPop7z -C\v4Ni-Ty*:DGB4f=zFmH vgXI=51/.-*'"#(-3;FTdu )BWenqpkcVF3 {eSC81,)'%#! &+/0.+'&&*.3564237@L\n-tj [2</e%iUJFDA<4)p= }iYLA82/--.//////012356777520.-.02321/-,-05=HUex.^f<v]J>62/--/7F`x|,F>gF0"      !O}: 1#uQ2q:       (K~g!)9[/   !$# TzaglEmAF`t>u>g Q6ZoQc b         !Mf+J;[3SO1nlPa|L  $%$   3~}dk&              b`n0B  !     >4K2+{d    c?GV x*      R vI        3d)&U #$"            >a/xZ*) CYQq+{      K32nc 8       X!{cS?y   0ev?DV            M'U]=d+      D-  X `2z5           6Z/y5&     OzpM      'eRH`%Sv?         3rVq_'=      y8AgfilF<|   %R]- 9o#b.   =O $:[i    E ,&P1E =2k1#!]K     'rhLu[: @Ax f'     N26||3wH-1"|  Z^E#  G9zUb*      jBh^qB      A6m5, )Z"IL';$${_ w   Pl7hGsgmmpw$    2UBC#Nte_D[sQ;Z    !P8yNQJ,% )V}n.=?p0   SRfi/ A  c\< <;sd*      N.C, TRc 7o.   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"     +%      '." .2!       /U&#  *$:"8P &* .A +:'-J  ')0-*" #'>? 6 '8,( 24&+ @3' "" )v,,4I*M# RZC4?=GJE(61e20Bnc3>Y0(;Y2,[@<=:E7TCW!NGeT(6:GJ W4<9n+;j|}(_HZATXwt0x\[]c}j7cFq?ZlLts:O\Wshc~zzSyPBjX}~zmQZOSx4ok/[ q*]35B"&p#N0" GGGAAGGCTCCTCGTAGTATATGATACGCCTTCTCCGCACAAATAATGATACTCCCTCTACCAGTAAATCGTGGCCCATAGAATCATAATGCCACCGGAACGGAGGGATTCTGCTCCTCAAAAACGGGGAGGTAGGACAGGATACTCCTCCTTCGATACTCCTCAGGAAGGAGAGGATAAGGATTCATCATTCTCAATCTAAAGGAACTATCCTCTCTAAGGAGAACTTTATCATGAAGAGTTAAGAGAGGAAGGATATACCTCGAAAGGGGGAGAGATCATCGAAGAGGTGTCAGCGTGAATTCAACTCGAGAGTAAAGGTTGGTACTCGGAGCTTCACTGGCTGAATGTGAGAAGGATCGAAGGGTATTTACTAGAAAAACGGGAAAGGAGACTCCAAAGAAGGATGGAAAGGGATAACTCACCGACGAACGAATTTTATAATAAAAACTTGAAAAATCTCCATGAAGGAATAAATATACGGTTCGGAGGTACGATCACTTTCAACACAATGTAAAATATAGAACGAGAGAGAATATGGAGTCCACGAAAGCACGGAGTCGTAATAGAGGGTACTTAAATATTAGTGGGTATTATAATGAGGAGAGAGACAATAGTGCTACTAATACTTCAGTTAGTATAACAGTCGATGTATAGGTAAAGTATTACGTAGTCCCATATTATAGTTATGACGCTAAGTCCGGCTACTCACATACTCATATAATAGACATCGCAGTACGCATATACACAGGTCTCACTCACAATATCATAGTATATCCTCGGGGGGGGAGAGGGTATATCGAATGGAATATACACCTGATTCCCTCGTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:28 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSyBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *UdDATA *Ud9DATA *UdaDATA *UdDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~wLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS:1P1AM>|ZP1RL>|TTP1WD>|aLP2AM>|nDP2BA>>tP2RL>|gPBAS>>=PBAS>>:dPCON>>QPCON>>MPDMFyPDMF:HPLOC>|G\PLOC>|@PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%:)SCAN3SMED SMLtSMPL pSPACAYٹSPAC:ASPACAYٹSVER4.0SVER y|SVER,,nScal@Scan3TUBEC9Tmpr<UserphARphCH:_phDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSyBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *UdDATA *Ud9DATA *UdaDATA *UdDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~wLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS:1P1AM>|ZP1RL>|TTP1WD>|aLP2AM>|nDP2BA>>tP2RL>|gPBAS>>=PBAS>>:dPCON>>QPCON>>MPDMFyPDMF:HPLOC>|G\PLOC>|@PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%:)SCAN3SMED SMLtSMPL pSPACAYٹSPAC:ASPACAYٹSVER4.0SVER y|SVER,,nScal@Scan3TUBEC9Tmpr<UserphARphCH:_phDYbigphQLcphTRphTRTrU]wfgM6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! P8X-o_}4]'G' YHT+(aQQxnJilXSXW .C=vz^`%C]Cp38V37gHr949q#ksl+&H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3g.W3DK]T5^.?s|"Ff61}XTu.ycI0 r#U43*n[l'ny#RqAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``B1PtB1PtBCTSyBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *UdDATA *Ud9DATA *UdaDATA *UdDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~wLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS:1P1AM>|ZP1RL>|TTP1WD>|aLP2AM>|nDP2BA>>tP2RL>|gPBAS>>=PBAS>>:dPCON>>QPCON>>MPDMFyPDMF:HPLOC>|G\PLOC>|@PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%:)SCAN3SMED SMLtSMPL pSPACAYٹSPAC:ASPACAYٹSVER4.0SVER y|SVER,,nScal@Scan3TUBEC9Tmpr<UserphARphCH:_phDYbigphQLcphTRphTR