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"-, +   #  & &             (  &$!!  )     )  $" %    '           #     !Ib         #       (,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d9e42e6ce5b5477ba96af7dce53df007Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-Well K1361_C12KB_3730_POP7_BDTv3.mobSeqPop7z)))*+-0233222346788777789;=>@A@?=;85310/.-+*('&%%&'())'$  $%%$"  !#%'(***)'$##$&),/012358;>????AGNW`it%UVe KV2Ng!v5~snnqy*iC3\wW62<;.D8G x`M?62258;>>=:754321.,*))+,,+)&$$%&'()*,05;AITf/`Kmz`2{CrVB50///./4AYQ9\~Dj`(Duee9          3j6x1KW$^;a)         #CqCl&lD&<oQ/* ?~V;+"   H>  ; R{i9  !"!9r:-wU  $''%"      $),,)"    78Ok(JvU   9sm90Z         *nWZ.J         bhiJT #DlmE#      /tM=)r>     #p# mf    D#GB7      f6v0cBIM-[ n|eb+            !#"   qyD@       #` b%  spELZ Cb (Z          %AI~  I"! 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(-).!*($#*$-((* '33*-. +'3(&# ).* #!( 0..$.) !..        ,% !+ '1:6:5:):'/-' />)4>>>>>>>>>6>8>/,68>>>>>>;>>;>>>>;;>;>>>>;;>>>>>"11".+;>;>>>>6+$-$1.>>>>>6>>>>>;;>>>>>>>>+11"++;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>;>>>>>>>>>>>>>>>>>>>11>>>>>1>>>>**$ *>>>>>>>>>>>>>>>>>>&83&$88.++*>>>>>88+2!>>>1>>>>>>>>>>>>>>>>>>>>>>>>>>>>>.>>>>>>>>>>>>>>!*&>>>>>6>>>>.>>>>>>>6>>>>>>6>>>>0>>>>>>>>>>6>>>6>>>>>>6:::::64::::6:::::4::.4)#::::::6::1%::+46'#6::::4:6::::.:::6:0:1'%# )'!*!/! 2*0'##%  '2+,-'% % "*%0%( .(!*+2& :.::0260:::'5'3::1!123(34*6'#5:+)':3(1:,4(& 6.6,"5)'6*3.6 2-3)".1$#6*-- 6%5)).6/(("(53 4/4%-6-!(* &'+4-") ( ! (-).!*($#*$-((* '33*-. +'3(&# ).* #!( 0..$.) !..     N=8?R !G?6989<8bQ@20:[P9AD3"7`[E\Q#KI]7 8[9^[A,)'(`?P V,$&<EPA9+F:[/G-8aLH?3W5Fa("?)S[ @4A7@[?(D12C`V.9RLYSA%U LG? D B  <L++A52>85+7cKbN"*7ON2]A=*U --KRD)IU4NQD@ @Z #? 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O$UB--E!:]P6H[ .-1+ UGBOa6.=3Q^'3"IXSTCN *V 6@"L%"81,SI&V3RI[2*H`)]2G3&S%+@T?!PQ T4@ &5MP\ EWY,_U53J.82,>,N8,T)8 2_N8:'!$-7 : RDab?2%a[T%.Ta?%D2 \:5-#_^bd-[]3_PFRY 8 9]FY` G`5Y3UT#B,FGY 8Y4A `=059O2$JD22I82I NO0S4 %<-36^B:L4?A#J20XE 2ZO?c>P& D'E,(?9H XS!D^*>e I'aNHGT~La8eg[SP. p {A1Qz05jv^#(sl2#@7N.d%o9- mQ/K!#4zwxqx'[c1{x\-Wj =U`}2AeN|d38/Y+ 6AlfAgXW;/ %R+b BT% M%%c;F0t:SMMgKH$+j.]EI,s5 ;0mvHGW`n4v 16-5. 83./ aJcZ[P'u Yv L5[%(V [{b5h0^R^.Ih .{K{N?3$&$tG=/w`-]F5)cCR %d*|U#C4> W aTw!6Os _ \ z-e(mwZSmIg  : 6 u [=hrn>D3^K n:37^+,_,F{wKQd3)Go |}i 5Y&RVnVQ0sP[(?m+k4$\K8`9PsJ^  hy{hfClk.%ndgPHp4bF_5T(@#%@t"L5 "@&( R7v"~mL k )  PreV j7}7t~}kviXXpF+pzFnC9@$"8F;?A8.7  ",# &                               $ ' & ( !"$ '#&!('&($ ,% 1!, )- ./',4./-0!Q< (,- <.-+ )0 966AF/0# %5/F@5HF9E4::/ 1(+(@@;713Y?CJ;-G79 =CRM5AE/1=3$CBB=8IGTPORG@D:0dcAAWE=5555OL65.5,])98>NWW<1E0zJ\bbbbbb>jj3C7eb?nGpbbTdka__W?L\Hh^\dzz~jdgpppnvlsxtsymoZ [tUuw$~N_M Mpz$r  &>>8>   $$$$$$>$$>$>>>$$$$$$$$  BPg9T=:pppp,*gyN|pY8pG_$8))[ Xd"^8+~IxU7$e'8Rp`jI,dpp~/ yhf+ sq}~Djp^:\jdPEaMMxgpIdvwpN_U VH%`e>vIyM*Fe3pi?v ,2n"z>F_DwM."Q/m1pgC;OAyp'ppdpppppppKdxp#d&wF^a~Q5[F<u4+"Nz3RL)B=xy8ZwD ' " /)!'D3@J/ /-(>3,A]+ )( # (!- P$$  "!  $   % (/!   "  ". $    u)$ &&$B"&"$$#- 2&N-L;&!$#$4%C7 3 48/=:+ #,5 k* /0, V#(.R<+(SM8J4M'&8++@;L21K[5)O-5!*J.%lU*)t%>,5,{P!(}|BOP + .YB'!grl@"k v^c/|tNv<w7jWK@K./Zl2ym6R~5H}W?I%2,Mc+8sx8PER|(wi29Q\rJU^|rDS:OQo{yc;Xl^}(hi !NvyZ3(o);ow1&E +y gsT E~'p}56,y) CCCTTCACGTGGTATAACGATATGCTTCCTCCGCACAAATAAGAGATAGTGCCCCAACGCGGATAACGGGGAGCGTATTCTCACCACTCCACCGTGTTATCTACACTCCTCCAGAATGGGAGGGAGGGGATACTCCCGTTGCTCCCACACTAATCAGGTAGAGGAGGGCGAGGATATCTACTTGTGCCTCCTCAGGGACACTCATCAGAAATAATAACTATCTCATCAAGAATCACTCGAGCGCGGAGTTACCTAGGAAGGGGTAGAGTATATCCCCGCGGTGCGAGGAGGTACTCAACCGGGAATACTAGGCTCCTTCACGGATACTCCCTCATACAAGAACCGATATAAGGAAGGGGCTATCCTGCATAAACGGGAAACAATCATAATGATGAGGAGGTAAAGGTACCATATCGAGAGTAAAAGACAGTATCATAAACACTGAAGTTGAAACCATAATAGAATATACAGGTGGAACTAACATACGAATCCTTCCTGGGGGCTGTTTTCTAGGGAACTAAAACACCCTTTGGTAGAAATATACCGATGGCTCGGGAACTCTTTTCAACAAATATTATGTACTCTGTCAATGAGGTAATATACTACAGATTTGATGATAACTCCAATAGTAGAACAGTCTAGGGCTCTCTAAAGTACAGTATAGTCTCACCTTATAATAATGCAGCTATGTACAGGTAAATAGCATACTGATATACTCGATATGTAAGGTGTCATATGTATAGGTCTCAGTCTCGATCTCACAGCATATTCTCGGAGGCACATGAAGTGTATTATATGGTATATACACTGATCCCCCCCGTTTTGGAAAATGGCCACTAAGTTATATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:43 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSkvBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6$DATA (QDATA (Q8DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~iLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS*P1AMPLP1RLPE}P1WDPRP2AMP_P2BAPPfP2RLPY]PBASPP.MPBASPP*PCONPPB-PCONPP>PDMFzPDMF*PLOCP8=PLOCP1PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%*SCAN3SMED SMLtSMPL pSPACA`~SPAC*SPACA`~SVER4.0SVER kkSVER,,nScal@Scan3TUBEC12Tmpr<UserphARphCH*phDYbigphQLcphTRphTR)This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSkvBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6$DATA (QDATA (Q8DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabyFVocyFWO_GATCFeat//yGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~iLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS*P1AMPLP1RLPE}P1WDPRP2AMP_P2BAPPfP2RLPY]PBASPP.MPBASPP*PCONPPB-PCONPP>PDMFzPDMF*PLOCP8=PLOCP1PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%*SCAN3SMED SMLtSMPL pSPACA`~SPAC*SPACA`~SVER4.0SVER kkSVER,,nScal@Scan3TUBEC12Tmpr<UserphARphCH*phDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;NR74@iO\f6!hqnKkv`Av?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7ZXmI.9LDo2($AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYCBO(TM::( RD2i`GxFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdA1*+[HPsOM 1jk[K2;+hj3@*7_62Q$OEH5 G,90lVSFsA bFX97z?&q6X+9mK\TI_aEdO{7+VDTj.uFPqWgZ*me2&dAr&;t[W?*b1j glX ,cW`$~+hj:gh_)!hs|O1^M6C,nJ3qo8-@MV^A$+v/y#PzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=KubQ2a50jj,4o-8N'nsv&'Y?sxWlZN.YHng=!AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTbbB1PtB1PtBCTSkvBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6$DATA (QDATA (Q8DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabyFVocyFWO_GATCFeat//yGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~iLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS*P1AMPLP1RLPE}P1WDPRP2AMP_P2BAPPfP2RLPY]PBASPP.MPBASPP*PCONPPB-PCONPP>PDMFzPDMF*PLOCP8=PLOCP1PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%*SCAN3SMED SMLtSMPL pSPACA`~SPAC*SPACA`~SVER4.0SVER kkSVER,,nScal@Scan3TUBEC12Tmpr<UserphARphCH*phDYbigphQLcphTRphTR