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"+ 0         B                                  '   (,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a47aab6ee5404626bbc5dc1e8851355aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-Well K1361_B12KB_3730_POP7_BDTv3.mobSeqPop7z ! !"#$$$######" !""" 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('#% +$(!!$)%$ +6A,7.5Y!555,)28)8)=++,%52219,?0B1;0:D?B<=><EEB:?F05D9@:<.9DIAJIt>O^C>ACPF?[E^^^DDXB?LGXKZ\AWVRGkXGeiRMPXkoNigj\WihSXYj\ieb`leohaikciaj_xzv|syrswxmq|vznpt?  `:V7 [vALMI (qE%4Z 1o1 7ptwvnr:'_Ly0Bb^-T$VmEqZtOC9 _"_8dagLd]?P{ uGp8@wz]pXpcWzU8O87)L@dJ|mhdp,z1f<Dd39}Lp8Fk)dG_)FFA{7;C&FpBR'}(`p5{]*!dy,KAp<ippap,pp~{Np"2p%Hdp\po8o.nQ"!9 ,F/ LdAGc98x\<Hf/7sb@ZRM7b' B/UiF/g/G!ahVjHnQNiohaS3RYg'LFf'Ub2T8ajl;rIXrRx^fO_7XK&<M4d >9*4sHwm- 1FhMc#9_|_m>L(i lDs fYo!m.o ebV -H0:J,_y],}qK'_ eH-AoYX\KnS u=d'1.?eRR$KmH^'pg\o ; osHc\rKK1\ZuFZS\Uu% XBX \QIO# NN^@Gm~Q83?3bBpm(,b=u ngKcUyO#6Y@$tP kB7iMu%=5j lrn wA?-7vbED`Pu~[53:HO`Xcg\qdT^#`DUM;  :LD?gS];YKT*G0b6^O( ICDr6H8R3\|X|m[3T7XI/^Q[Gfp,m4,]kjM0h7?"#$&((CW! 0&) :Z47$%&7!L9, 4C)<FM#;E*;5 $5!'Y-!G".,-OQI@$"Y)\9[$9'#6<:)XG+(MM%,bMMlI(8)D9M3457o J$LBQK<G^nD%Yu;9FcfnhpE3KMg L2:liMyXCd_|D[KQFtQ2~ ]L.zekkQch3rW {\|ylMlx  o @8-hD_^hXtEm9h*w  CCAACTTCCTCGAAGTATATGACACGACTACTAAATACAGATAATGTTGCGACCTCTACTCCACGAATATAAAATTGGGAATACTCCCCTTAATTCCAAGAAACCAATTTTCCTTTATTTCTAAAAAATAATTCGGGTCCCTTAAACATCTCTATGTTTCTGATTGGTTATCCGTATCCGTCCTTCTTAGTTAACAACTTACCTATTCACGATGAACACCTTCTGGAATCCTTCTTGGGGGAAAATAGGTCTTTGGGGAGCTGGGACTACTCACCTTCTTACCAACTCGCTTAACCAATTACTTTCTAGCTTAATAAATTCCGTATACCGGCCGGCACCTAAGCCAGGGCCAAGCCTATGCCAAGGATCAGGCTGATTGACAATGCAGGTGATTTTTCCCCTCGCCCAGACCCGACTCGAAAGGTAGCCGAATCCAAACTTTCCAACCGAACCTTCCCCTCTTCGCAATATTCTTCGACTATCTTGTTAATTTAGATCTCCTGCCCTATATATAACAGTGCTCCAATGTATTCCACGGATACACGCAGACGTACGATAGGGTACGCACCTATTAGATATTATTCTAATTATGAGCGCTACACTCGTGCTACTAGTGCTTCTGTTAGTAGAATAGTCTATGACGCGATAACGTGTCACATAGTAATACATTCTAATAATGACGCTGGGTACAGTTAAATAACATACTCTTATAGTGGTCTTCTAAGTAAGAATATCTGCAGTTATCACTCACAATAATATTGTATATTCTCACCCCGGGAGAGCGTATATCGTATGGTATATACACTGATCCCCCCCTCGTGATGAACACAAGCGGCCCCCCCCCCCGCTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:45 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSl_BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6PDATA (QDATA (QټDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~jLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+zP1AMSLP1RLSFEP1WDSSP2AMS`P2BASSgP2RLSZ7PBASSS/PBASSS+PCONSSBPCONSS?PDMFzPDMF+PLOCS8PLOCS2SPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%+rSCAN3SMED SMLtSMPL pSPACA` SPAC+SPACA` SVER4.0SVER lTSVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCH+phDYbigphQLcphTRphTR%This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSl_BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6PDATA (QDATA (QټDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabzFVoczFWO_GATCFeat//zzGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~jLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+zP1AMSLP1RLSFEP1WDSSP2AMS`P2BASSgP2RLSZ7PBASSS/PBASSS+PCONSSBPCONSS?PDMFzPDMF+PLOCS8PLOCS2SPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%+rSCAN3SMED SMLtSMPL pSPACA` SPAC+SPACA` SVER4.0SVER lTSVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCH+phDYbigphQLcphTRphTRMr,c],~:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;NR74@iO\f6!hqnK(v1VK?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7ZXmI%9LD12($AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYCBO(TM::( RDji=j!FlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdA1*+6HPs[M 1jk[K2;+hj3@*7_62Q$OEH5 G,90lVSFsA bFX97z?&q6X+9mK\TI_aEdO{7+VDTj.uFPqWgZ*me2&dAr&;t[W?*b1j glX ,cW`$~+hj:gh_)!hs|O1^M6C,nJ3qo8-@MV^A$+v/y#PzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=KubQ2a50jj,4o-8N'nsv&'Y?sxWlZN.YHng=!AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTbbB1PtB1PtBCTSl_BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (QDATA (Q6PDATA (QDATA (QټDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabzFVoczFWO_GATCFeat//zzGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~jLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS+zP1AMSLP1RLSFEP1WDSSP2AMS`P2BASSgP2RLSZ7PBASSS/PBASSS+PCONSSBPCONSS?PDMFzPDMF+PLOCS8PLOCS2SPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%+rSCAN3SMED SMLtSMPL pSPACA` SPAC+SPACA` SVER4.0SVER lTSVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCH+phDYbigphQLcphTRphTR