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 %   " $  "                          %          $++" %                      (,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRWdhiiiiiigfeda`][YXUSPNKHFB@>;9632/```VVV{{{!!+--9:9EEEPPP[[[feeppozyy                                                                                  <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 603552030107484095da8999ab90b3faSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_18-57_05723130Seq1228712287_Costion_K1361_matK_fjmadera96-WellK1361_A5KB_3730_POP7_BDTv3.mobSeqPop7z76653100123321/.../023455543333456777766530.--/36763/,+-03553/,)(()+++****+++)'&'),/2569<@DIMQV]gs+:ENXbkrsnaP<(}wrokhea^[YXZ]adec`][[_cgihebciuH4Z2H}L|2n`bq  j9kQE=Gu$W}RkXNHD@<98;?DINU_p1k$W|f< UiUIB?=<=CPh hZvpuxRRe<"            ! 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V7K+MK'* ^E@S%! :A J 1^>W*FBX /QAY6]9;U6NCdDa X"$ 7 /L /MHOP52b/)^]P>:;V@.26Y&52/?J7FZV6L D2223%ZOW.6.XG 6.Y5/VFSZ3.6.136X  (^2IH*5GXH2L^/2FGhO"Mm  mN^E,4;NAu6E2x B ANUSsJGFdo PCf YX9 4M"dsk$ l!t|R {=(0| d [D;1Ec G}@ml'5+8 Xz>U3.!})onQJDGx9$#T= CZjEoN43OHAby<WkM.B#S%0%y"y[]rDHuo2blVpXV|,Iypv/_'MHEKz"xSwHs]3'18RhLh52B&xVI(mz>CSX-"/QqONpqGS?,B{:3|[~?IZ"^H7  ;/N }q)/ uExjydsT7Fg6oIM"K>WQ>zOKu ,R#bE0_hl~}ML[n2"='E{VeD9%N_7<zOLu,}L[KSsO+Yb8Z2rD@DE({ MgnL`H)Z 1: w 6R BG e2c #6i|otaaU_lbe[kYJ_hXl=-,!4/'"3,?*@(    #$      xxxxxxxxxxxxxxxxx              $ "   2 (+!),!"1#!' 6I(<4 ";+ 37*32';1,%*?6$'*52=G=4E626}:9M)5LGSB576?O:;6O7LL=:FIWDK<____IR+B:VV,OkSBOMg{\QQOL`PYu\`o+cb]`_SmQi_u^hhkhwprhyn}vldehxty}vytzr~z'm6. $XML:C<2*1 =v p~ -:aapMJ8?PK=JO9/9S!N}pj3^e:nOH}\r`BN78|8d|mGUuuL-pdvaZLefbN|~~JH:5I^3+';Y,X:E8*C((^/3m0&GL$@46<&Qs>]v0xYP#qxjWo/?=f:#I1G08|Q;qXIu<m}kRIYv[|T`cjl`UJ'~vIAaHI~2[QCJwb[Rcn0bqxY:P"!>T`oo!BJDP Wf%a7ENK!iML<+i #D5CCAACCCCTCTAAGTATGTGATACGTCTCCTCCCCACAGATAATGACGCCCCCTCCATAAGTATACAATGGGCCGTAGCCTCCCCCCTCACCGGGGGGGTTTCCCTCCTCCCACACCGGGGAGTAAACGAGGACCCGTCCGCATCCATACTACCCGGGAGGGAGAGGAGACGTCTCTATCACTTGCCCTCTTCAGGACGTATCCTCATAAACAAGAATCACATCCTCAAGTCTTCAAAGAGATAGTATCTACCTCTGAGTGGGGAGAGGTTCCCCCAGAGGTCTCAAAGATGTATACCCTTCCTTCTTTCTGTTGCTCCTCGAAGTTTCCCTTGCTCGAGGTCCTAGAGAAGGAAGGTTATTTCCTCTCTCAACGGAGTAGTCTTTCAAAAAGGAGGAGGGAAAGGGACTCTATCATCTTCACATGAATCTGTATAACCCGTGTCCGCACCCACCAACAGGGGAGATACATATGGTACGAAGAGGAGAGCAATCAACAGTCCATGCCTTCTAAAAAACGGGTGAGCCTATTTATTCCACAGTTGCACAGACACGTACTCAAGTTTACTTACCTATTAGAGGGTATGATAAGGGGAAGCGTTATACTATTACTTACAATACTACAATTAATATAACAGTCTATCGCGAGTAAAAGTATTATGTAATAACGTATTACAATAATGAGGCTGTGTACGGCTACTCATGCTAAGTATATAATAGACAGCTAACGGCGCAGATACGCATTTCTCACTCATAATAACAGGAAATATTCACCACGGGGGAGAGGGTGTATCGTATCGTATATACACTAACCCCCCTCTCGTGATAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 20:28:00 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSvBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *\TDATA *\T9QDATA *\T DATA *\TDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~u(LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS7P1AM<xX P1RL<xQP1WD<x^P2AM<xktP2BA<<qP2RL<xdPBAS<<:PBAS<<7PCON<<NXPCON<<KPDMFyPDMF7PLOC<xDPLOC<x>,PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%7ySCAN3SMED SMLtSMPL pSPACAZ{SPAC7SPACAZ{SVER4.0SVER vSVER,,nScal@Scan3TUBEA5Tmpr<UserphARphCH7phDYbigphQLcphTRphTR(This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSvBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *\TDATA *\T9QDATA *\T DATA *\TDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab FVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~u(LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS7P1AM<xX P1RL<xQP1WD<x^P2AM<xktP2BA<<qP2RL<xdPBAS<<:PBAS<<7PCON<<NXPCON<<KPDMFyPDMF7PLOC<xDPLOC<x>,PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%7ySCAN3SMED SMLtSMPL pSPACAZ{SPAC7SPACAZ{SVER4.0SVER vSVER,,nScal@Scan3TUBEA5Tmpr<UserphARphCH7phDYbigphQLcphTRphTRFj6%n?oX?HYTw|bT|MI>"oCTRM*?Bcz6F&ZC'nUz}%?^`0L)^,KUS|Xq:u0#|UR7\6|y9o31)-PA)y1XV7:>JMB*u $O6T-jpjK4|<"G+SP7T]Zjp7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/&({T?HK_eXjKy#Id`Po<_+MWQ(SbXW,VE+,[Ol% eIe]`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+b-M3lLhZ+,E^W?|QD/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWDZOv'5d7`Ed,{w.P&-Gt?E>@d&r&iox~*4fC\*WiE&Li58&obFMR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4[42g|5PxKoJ-[CW?R5Ch2d'jw~|>J3zi^{g5AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``B1PtB1PtBCTSvBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA *\TDATA *\T9QDATA *\T DATA *\TDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab FVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~u(LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS7P1AM<xX P1RL<xQP1WD<x^P2AM<xktP2BA<<qP2RL<xdPBAS<<:PBAS<<7PCON<<NXPCON<<KPDMFyPDMF7PLOC<xDPLOC<x>,PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 9RUNT  RUNT  RUNT 2Rate RunN""S/N%7ySCAN3SMED SMLtSMPL pSPACAZ{SPAC7SPACAZ{SVER4.0SVER vSVER,,nScal@Scan3TUBEA5Tmpr<UserphARphCH7phDYbigphQLcphTRphTR