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",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRA]chiiiihgfdcaa^\ZXVTROLJGEB?<;7631```VVV{{{!  ---:::EEEPPP[[[ffeopoyyy                                                                               <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 263217e76ba140b498b5126f035946b3Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_17-26_05713130Seq1228612286_Costion_K1360_matK_Rjmadera96-Well K1360_D5_RKB_3730_POP7_BDTv3.mobSeqPop7z  "',03568<DMYep{ {rjbYPG?82/-,+)&" !$'('%" #&*,-,)'%#!  !(4F`#m% d  w E>6_tWA1&     /Jo lpFgo[-/m;q@c{]9 KcM "pqm>"    "&))($ *R!{(jP>\3    !X/Boi6y&X$ 0aO&<2ozD   *Ye<}+UQ%     !!       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"*"8"E"Q"]"j"v"""""""""""# ##%#2#@#L#X#d#p#}##########$ $$#$/$<$J$W$_$i$|$$$$$$$$$%%%"%/%=%G%V%b%n%|%%%%%%%%%%& &&1&>&J&S&\&r&&&&&&&&&&&' ''''4'A'N'Z'f't''''''''''((((-(:(I(V(c(n(}((((((((())))))6)C)M)V)_)r)|))))))))))* **%*3*@*O*]*k*v**********+ ++(+7+G+W+b+t+++  $$$ :1 '$*:1%6":::'>6> >.>>>/>>>>>>>>,*>>>>>>>'>>>>>>>>;;>;>>>>>6>>>>>>>>>>;;>;>>>>>>>>;>>;>>>16;>>>>>>;>>>>>;>>>>>>>>>>>>>>>>;;>>>>>>>>>;>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>;6>>>>>>>>>>>>;>>>6>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>6>>>>>>>>>>>>>>>>>>>11;>>>>>>;>>>>>>>>>>>>>>>>1>>>>>>>>>;;>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>6>>>>>>>>>>>>>>>>>>3>>>>>>>>>8833*8/+)>>>>>>>>>>>>>>8>>>>>>66>>>>>>>>>66>>>>>.>>>>>4>>>>>>>>>>>>>>>>>>>>>>>.>>+>>6>:::::::::::::16::::1(:::.:::.::6::%:::::::4::664/:66::::::::/::6#::::3:::: :):1':2::5:/:34::.: :42::5 +5::553::1*13::::):2:(5::56355::*,),:: :12:::::',)66636663,,1666(21 (31'&565465.,*6*6666)4454&#44"5 *5 454!-%$5!%5" -5  3 !3/.!*3- 3%*+& 0 ! 0)%&!**)"% !$/ '00.*"*" #%'& +#/$/&& !$ * ,,,,+     $$$ :1 '$*:1%6":::'>6> >.>>>/>>>>>>>>,*>>>>>>>'>>>>>>>>;;>;>>>>>6>>>>>>>>>>;;>;>>>>>>>>;>>;>>>16;>>>>>>;>>>>>;>>>>>>>>>>>>>>>>;;>>>>>>>>>;>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>;6>>>>>>>>>>>>;>>>6>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>6>>>>>>>>>>>>>>>>>>>11;>>>>>>;>>>>>>>>>>>>>>>>1>>>>>>>>>;;>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>6>>>>>>>>>>>>>>>>>>3>>>>>>>>>8833*8/+)>>>>>>>>>>>>>>8>>>>>>66>>>>>>>>>66>>>>>.>>>>>4>>>>>>>>>>>>>>>>>>>>>>>.>>+>>6>:::::::::::::16::::1(:::.:::.::6::%:::::::4::664/:66::::::::/::6#::::3:::: :):1':2::5:/:34::.: :42::5 +5::553::1*13::::):2:(5::56355::*,),:: :12:::::',)66636663,,1666(21 (31'&565465.,*6*6666)4454&#44"5 *5 454!-%$5!%5" -5  3 !3/.!*3- 3%*+& 0 ! 0)%&!**)"% !$/ '00.*"*" #%'& +#/$/&& !$ * ,,,,+   8;&M);cE cKI0Z;2`DX,P./a'9'^ LE2/V: [) 5^?/*%6IMIA1N*O6(9V4EB>4IK+K)$V^!CFT !DZBJ8_-?+L` 4'U+5A$B%KM*T .NR;b!T2da4%]M?OZYN9.HZ _%V">AVG2$(0W ]CAQ@!5+"NW!+NL)XS&@&`!@\ D7/M]K1_V<*#7M=QIC^SH(=HV*;a#;?$I5F<P$8ES,U1^IS4T<4ZE#PL\C(B 3P%[Y-\KD8'7I,)*`6O='%?E;JNOJ32 "(OAY9'5UC9*GZL;%(1D/B)G <285>Q8 PO[ WUP CAGDY$23F[/ Ob7M$I$"8\';Y7P2>D A(0dDCF1^9]2 Ba/4()"5P0@?+(XG)= &< :S@FEZF,!3N>67GH B92J?L25)R\1.X(*,8)L3#]5%1*^+\R/@#F9[ C =_QQ[<P?T!MOC*+*AdVG+ I`;+.TA='cG$KI#='%-B >3CX?Nc-c8= _&:9 5b%UB(KQ?)$S2&%+JA0V*%\5F "2BQO?U.[6E\.A@dH-LW%2` +VG9\&* CN+9WT;CA=aN R [6X)Z [C2 Z)VI !b @$W` [ @R;&>)'=J8,-4 $H&B&>A'=8,'=YO&2>8N\2# Sa2W <$W` 2Ab2?`2ZGS5L/9]WDaK!aab7LN4N>O&aW caW a *%D=#x.8PU_ P(P+d}v .J*M5_~%H*)wPh&AG [Ejwy:rd9T9TK9C=) v!<$^tau% PR'u(iKU4A lhj %t)^U .IC8[quGTTp  #Rc as0RJV h}bZ}_"z>['i vTl>t4Hj`;<5vj(6% ?o%Q)Bh?1[elY8?[;cq'U-,#vkjhJ=Q}E?KeZ^ej[8_!^X4pc  fI1 x|L9TPl_Yy P>@mj2, ==HJ=TRD>B|3  +KXKlic2O0O$ Ovj (L9.v>Rvjvm/`NvhDH4O;q=yy' Wt5#_ 9 {V#m!YWQJ 1'r=_Pa$N$j?hw ?^;x5^I6)vY].'41iOA9[.Fa{"NDI:elojYDC H=d4&X;mERa>k\}yGL7fH.]*((:&@3> :& +% &,#'             +!  4   .+,  "! #%("$#*$'*-$"'"+"16 83*-<#4#/+;&/,,-',/DA"1)8!7!5@-3<J$.#05Mb-C&D%1.=1BQGJJX[GRFwo]qY?JJPfPj]QWPG\LZXXXcZVUTeZk{[W^damdSjvbiiiioYyXriivzqlf|setscvsrw}tlur2ttu{=9/GI3,,E!%,,0,, Z[Z[[ZKKKH1KKKKZ5ZZ'KZ8Z[ZCZ[1#[Q%K[[[ZZ'[[[.B:[[7[[ZZZO^nnio[[[[[[KK[[[[[KK[[+A~jpppppdppXp/r8Ly's#58w1Eud[Vd|d:Ddp` )7_3$+6#B0id<pi%FR.8{8T9E][8dpx}db;pBppsk|1jeN<QYK~d`~,pY1g?%^j,?88;()J)n1ls}pppppipp[, ,Iuppp'pppppppp_pppppppppvpap"a^K% `5fo@Ugd2-5W!!'7]6\p3#*/jD&'&4,! , )   D4# # !% %!      $  1+ %     #        #    0(   #      $# *!"&"' " /tS0 !I0(#b %!!1%5/ *  !0! $! E 0"(Mh&(#+U)-/?f#/96;;!(#L^=> =E/%3HR>@ /R(-/9A'C'Xl7l5-1p?k!8.P%/IS/"w5D2QKm9XKD5;HO.rO]L"kK!"QRSR(L$Er;p'`RO&J [L a  4PP %cGgT6\icnm}g~WduZvgfJjY~b8[pr5e(f ^ E8 PV_bXhjBScs#X:}bsVL>ZwFbZ 1C\4  Dl,hU$}Gk TTTTTAACAATTGTGGACGGGAGAACAAACAATGTTATTAAGAGGGATACCTATACATAGTCCAACCGATCGCACCATTATAACAGGATCCGATGGATCTCAACCGGAACTCCCACAGGAAAGGAGGTTCCACACTAGTCGTTAATGTTCCAGAGATGGAGCTACTATGAGGGGGATGTCATAGGCAGCAATATCTTCCTGTGGAAGGAGAGGGTCTAAGAGGGAAGGAAGGTCTGTCAAGGGGCTTCCGGTCCTCGGGGGAACAAAGAGTCGTCCTGGGAGATGATTCAGCCTAAAGGGAGGGAAGCGAGGGAGGTTGCATATAAAGATTCTCTGAGGGAGGCAACCCGAGAGGGTAGTAGACCGTCAGGGAGAGGGGGGAAGAGAACTACACCTCCTTATGAGGAGAAGAGTTTCCCAAGGATTCAGTAAATTCCCTTCATTTTATTGAACATTAAACAAGGGAATAGCCGAGGAAGATCAGAAAGGGTCAATGCTATTATGACCACATTAGGATATGAGAACCTTAGAGTGGATAAGATTCAAAACTTCATAGGTATAACCTCGCACGAGGGGAGCTCGGGAATATGAGTAGTGTTATGAGGAGAGCGCACAAGGATGGCAAGTACAGTACACACACACATACTCATTATTAGTATAGAAGCAGCGGTATAGTACAATAGCATACAGAGGGGGAATAGTATATAATACTCAGAATTGAGGGGGCTATACTTCTAATAATATACTTGTGGAGGGAGTATCACTAGTACGCGGGAAAAGCATATCGAGTATTCCACGTGGGAGAAGTGCAACGATACCTACATATGTGTAATATACTATAATAATATATCTTACACATCTCGTGGTGTATTATTAGGTCTTCTCGCCCCGAAGGGTGTAGTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 18:57:16 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +WDDATA +WD<DATA +WDKDATA +WDDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISAP1AMeTP1RL^FP1WDlbP2AMz~P2BAP2RLspPBASEPBASBPCONZPCONW8PDMFPDMFAPLOCP*PLOCIPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 8RUNT 1 RUNT 8 Rate RunN""S/N%ASCAN3SMED SMLtSMPL SPACAXjSPACASPACAXjSVER4.0SVER SVER,,Scal@Scan3TUBED5Tmpr<UserphARphCHB phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +WDDATA +WD<DATA +WDKDATA +WDDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISAP1AMeTP1RL^FP1WDlbP2AMz~P2BAP2RLspPBASEPBASBPCONZPCONW8PDMFPDMFAPLOCP*PLOCIPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 8RUNT 1 RUNT 8 Rate RunN""S/N%ASCAN3SMED SMLtSMPL SPACAXjSPACASPACAXjSVER4.0SVER SVER,,Scal@Scan3TUBED5Tmpr<UserphARphCHB phDYbigphQLcphTRphTRKj5)l#O,[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:T}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@Ft/rp<.3#+Fs:$WWV5[z7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKb0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme