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%                  d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333G>GRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRREWeijjkjjjihfdca_][YVTQNLIEC@=;8630.`a`VVV{{{   ---999DDMPOZ[[eeeoooyyy                                                                          <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5b6f25811f204e13b335027e2c7dca24Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_12-45_05533130Seq1228112281_Costionjmadera96-Well K1359_G2_RKB_3730_POP7_BDTv3.mobSeqPop7zqvC5wl V Sh "&) +.1F369="@CGMJMPSWY\_behHjlnpHqrtu7vwyuz{|;{zyweutsOqoUlhc_ ZW S`OKcFB=>840- )&B#0 u){)$ POAF%f\X+/Pq}u"TsX ~Wukrc}~   # # w 8$@f9HGuB'&>gDp,q)aoh\k!m>$Nh#|W/p03Tp`0|{ =m I\b"8kZ\n6t`b& k+~< >:c$++("c\` [!5y)=MPC$u`MBG]}{T)\0&&  .WT*"<Wjqj\PLTcr{zqd[\jC"RpJ(k0 "hh oY"T^v^UTQH4A7yxcH3ZFW #dq]1{fG'*TS& *|y[O%wRq) 9ta0Uxa<  (Fd{~?zQ#+sY}, F#enk* #Q51rJ[o'!8PcnndQ:$ 2n2PR=M!7Uc[@ /j-D:P! 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(q@"A A@A )KB.bcpKB_3730_POP7_BDTv3.mobtermGGCCCCCTGTGATGAGTGCGCGCAGCGCTCGATCTGTATGATTATTGCTTAAGATCCCAGCATCTTTAGTCTCTTAACATCATCTGATCCTGCCCAATAATTGGACCCCTAATATGCAGCCCCGATTCGGTACTAAATTTATCTTTAAACATCCATCCAATGGGCAGGAATGACTGAAACTATGGAATCGTCTTTATAAATACCTCTTTCTATTAGGAATGAATTCTCCTTCGACCGATTCCTTACCACCAAAGGATTTCTCCCACCACTTGAAAGACCCCCACCAAGATAGAAAGAATAGTATGATAAGTGGGTTATATAACTCCCAGGGTGGTTGACACCAAAATTGAAAATAATATTGCCTGAGACTGACAAGGATACATATCCACTTCTTAATAAAAACATGACTCCCTTTTGAAGCCTTAATTTTTTTTCCTTGATATCATACATAATGTATGAAACGATCCATAACCACCCATACAGTTTTCTGACCATGGTTACGGTACCTTGGCCCCCTGTGATGAGTGCGCGCAGCGCTCGATCTGTATGATTATTGCTTAAGATCCCAGCATCTTTAGTCTCTTAACATCATCTGATCCTGCCCAATAATTGGACCCCTAATATGCAGCCCCGATTCGGTACTAAATTTATCTTTAAACATCCATCCAATGGGCAGGAATGACTGAAACTATGGAATCGTCTTTATAAATACCTCTTTCTATTAGGAATGAATTCTCCTTCGACCGATTCCTTACCACCAAAGGATTTCTCCCACCACTTGAAAGACCCCCACCAAGATAGAAAGAATAGTATGATAAGTGGGTTATATAACTCCCAGGGTGGTTGACACCAAAATTGAAAATAATATTGCCTGAGACTGACAAGGATACATATCCACTTCTTAATAAAAACATGACTCCCTTTTGAAGCCTTAATTTTTTTTCCTTGATATCATACATAATGTATGAAACGATCCATAACCACCCATACAGTTTTCTGACCATGGTTACGGTACCTTEMhnx&6HYds %06EU\hw '1;JT\iv"-:GR]iv!.;FS_mx!+:GR^lw+4DR^kt(/>JR\iv    ) 7 E P [ f s    ( 6 A L X c q |  # . < F R _ i x    ) 3 ? 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(G'B33&78$ =6 .0!J +J9NS$+DG0_:`761]J"aI=!]8 B ='V@=._L a?>*>L]'A %?I%",EU28&GNSO)NI 7<*(:3W$G Bx#!tK xKO!4Z2F_n9fU\udt Lf1 ZYmX6k   'f`Keec5f]WioV8zX"2I{gSMSX.xv>NQ90% B 0m,j:C5QB]lc7\d \t9_!]J]~O`d |Qki<nmcI1Wt &k3lc c)H21}W;x cT^XkMSiKi jd,GE^H9xeRYAqoZ^*/T ma]uV |4 2+Nvzzt;qtBG uAg6uj}fL Im HHs ( `"+=D2Z*6H?4U"iR7M3Qx8%5J'l WTB7 4=@F'; 7#=%A   ,A6 *E-X= !!I #!,$&2%Di,_.q>'.Gr ]xf2G1!^he</+2 F K .'l, Jp0p)o^sSh*/WJ|FF=:i&DZ"$BOT])n{A,l=('d.Pd*{Uj8pKq!K[Js#/o6W k,_ Gl4^Xb-4b?PZawxpOP-y@~[wE;{V\jS8=yhN_ a{kn%J@ dO ?*r~}>V;vS}d:V(B>:0[7,\=ZAdDL][v2rplK} pt{_`JTq:Ag"> w<w 66%3_/M~*S%JqO3Wg2Gt >\vuYh .2<Bz\ ,0\Z`#ELX lPLeov5il=@-"eo|dhF y]h0 ^O [#~T|< [0)X"!Np=#OEw&'u RH>u;t2uymVSy3sHw`T`Hl,1?6FpILT]:cM@2sZtcj*r=c<\*$5},_Q9|? )wOGH>(j/YqmI}^t_JOB(|KqS:z]S [H&'TAAAAAAAAAATATTAATTCAATGCAAAAATTAGGCGTAATACTAGTAGACTTGCGAACCTTAACCAGACACAACTTAGCGTGTCCCATGATAGTCCCCCCCTTCTTAAACCCTGCACCTAATCCGATACAGAATTTCAATCTGCCCTTGCAGGCAGGCGAAAAACTAGTGCGGACCGTACTATCTCATTAACCACATCCATTAAGGAGTATAATTATGGTTTCCTGGAAATTTTTACGATACAGTGCATGGCTTTCCCTCTATGTTTGAACGTCACTAAGACTGCTCGGGTCCTTCCGCAGTCACATGTGTTTAGGACATCAATATTTTCGTTAGACGCTGGGCCAAAGGTTCCTATCCTATATTTTTCCGTGGATTATTCCTGTTTTCCTCACTACGTGCGGCCCGGTTTGAAGCTCTAAAGGCGAGCCCGATTCATTCTATTACATGCTCTATGCCCTGTTGGAGCGCAATATACGTACCCGGGAGAGAACCACAACACACTTTCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 14:16:04 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSdBufT'NGCTID#CTNM)CTOw7CTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA +V(DATA +V(:DATA +V(DATA +V(8DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~cbLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS=hP1AMQ}P1RLMP1WDUwP2AM]kP2BAaeP2RLYqPBAS?PBAS=PCONKPCONIPDMFPDMF=PLOCEPLOCAPSZE`PTYP?PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  -!RUNT 6RUNT  RUNT ,Rate RunN""S/N%=`SCAN3SMED SMLtSMPL SPACAXySPAC=xSPACAXySVER4.0SVER dSVER,,Scal@Scan3TUBEG2Tmpr<UserphARphCH=phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSdBufT'NGCTID#CTNM)CTOw7CTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA +V(DATA +V(:DATA +V(DATA +V(8DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabsEFVocs4FWO_GATCFeat..sGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~cbLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS=hP1AMQ}P1RLMP1WDUwP2AM]kP2BAaeP2RLYqPBAS?PBAS=PCONKPCONIPDMFPDMF=PLOCEPLOCAPSZE`PTYP?PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  -!RUNT 6RUNT  RUNT ,Rate RunN""S/N%=`SCAN3SMED SMLtSMPL SPACAXySPAC=xSPACAXySVER4.0SVER dSVER,,Scal@Scan3TUBEG2Tmpr<UserphARphCH=phDYbigphQLcphTRphTRom;PsqD57%6C}:*SM|aZA /GAjrS$I),_A#$O7{_O[T6S$gI{cTKcaza(RO[mOEW<#GnP Qc$@)kOie&>2 xX|L=6XO?Tve!@;wW14^M1}Gu9v_QjXa*`)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lA^5ehJb@4}%P!SNmWT/OKOvx4G!?`U!9@mwyy>T3pb}045@u:r*nOrozc{G[ZI,|Q|T{yK{1P3;fTR[fRr;-YVJ|Q:{+ z9X=5>2jDTWy2>y^q3Th$Y thENWNv3[My J1\QWi.HhrjcngzE<=#d1a-cF~bvHN+MzL6L!<7J8_M4;-6`Zdg~P_F7a;A{%ipoWeV&jJDeVE+hj3EYWC.%obB`!3xrM "K_l#zjCAzh`u9;lT'8A 0wz`&1ZG|JW/e2FVc2(_CSR{[}7"=Iw<1C{_@6tKR"\pI2r>6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JXs2M*;N/VMR{7gmUo:e.s$;_CP)DzEC>3f9bb14=M=IOd;HA>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_^Z0b>"EJX.=DaJ2`>nX$t!TQqvLWFq$~EZ%+K]`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtAUDTddXB1PtB1PtBCTSdBufT'NGCTID#CTNM)CTOw7CTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA +V(DATA +V(:DATA +V(DATA +V(8DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabsEFVocs4FWO_GATCFeat..sGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~cbLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS=hP1AMQ}P1RLMP1WDUwP2AM]kP2BAaeP2RLYqPBAS?PBAS=PCONKPCONIPDMFPDMF=PLOCEPLOCAPSZE`PTYP?PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  -!RUNT 6RUNT  RUNT ,Rate RunN""S/N%=`SCAN3SMED SMLtSMPL SPACAXySPAC=xSPACAXySVER4.0SVER dSVER,,Scal@Scan3TUBEG2Tmpr<UserphARphCH=phDYbigphQLcphTRphTR