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J 2 0 5 ( 9 0 C @ M @ V B Q T L U Q c K [ b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, 2 / * % 4 1 3 ' > 4 / , H A = G B ? : - 1 3 9 A E 7 B ; 8 ( + . 4 , + ! ? % ' # < & 9 , * 5 , % 5 & $ 7 % + 9 # % ( / - " % ' , ! 6 $ , ) = - 6 / & ( 6 1 - " - 7 A / ( ) ( 0 8 ; + 4 2 8 > > + / @ 0 / = $ 2 ( ) 1 ) 1 / , 3 - 8 # 0 # 1 / , 7 8 ! + + ) " ' * 5 % + * : - ( ) + 2 + & ; , d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333G>GRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR EWeijjkjjjihfdca_][YVTQNLIEC@=;8630. ` a ` V V V { { { ---999DDMPOZ[[eeeoooyyy
< < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6bf659cee30c49fca8e5cfc145c3990fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_12-45_0553 3130Seq 12281 12281_Costion jmadera 96-Well
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?> #AAGGAAAAAACACTTACGTATAGCGAGCCCCTCAGCGTAGGGGGAAGAAATTGACGCCATCAGCTTACTCGAAACGTCCCCGGCCAGCGAACAATTCGTCCACTTCTGTTTAACATTATCTGACGTTGTCAAATCCCAGACGTTTGACGTTTAGGAAAGTGGGGTAGTTAGGCGTCACCTATGCGTGATTCGGAAAGCCCCCCCACCGGTGAATCCCCCGATTTTGTTGAGTGGAGCTACCTAGTTAACCCTTCTCCTTTGTCATTTCATAAGTCAGGAATCCCCAAGTACATCAATTTTCCGTGGGGGCCCCCCCATTTATTTGTCCCGGGGGATCGTACCTGGGAGATTGGAGTTTCCCATCATCGAGATAGAGTTATCCGCTGGCGAAAGAACTTGCAGAGAAGAAACACTTCGTTCAATGTATAATTTCCTCGATCCAGTTTGTCCTATAGGCAAACGTAACCCACTAAAAGAATCCAAAATACCTTCTGCGGCATTCAACGGTTTTGTACGCTGATGACGATCCCTGAAGCGCACCTGGGCCTACTGCCACCTGCGACCGTCTATTTGAACCTACGTAGAAATGTCTTGTACAGAACGGGATGAAAGTAAAACCCACCTCATATTATTCACATATTAGGTCCTGTTTACACTCCTTCCCCCCTCCTTTTTAGGATTGGAAAGCCTCAATCGAGCTGCGGTAGTCAATACTCTGGTCAATGAGCAATTTGGATGACCTAGGTGAGTCCACACTTTGTGCCACTCGGTACTAGCGCGGAGCGTTGTCTACATCTATGAAGATAGACTGGTGGAATGTCTCTAGCTCCTATGGCGTATGATTATTGTTCCTCACTAATACTCTCATAAAATAGTAACACCAGTCTCCTATTAACAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 14:17:03 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt v ASPt v B1Pt u B1Pt u BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +m V DATA
+m V ; DATA +m V t DATA +m V N DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE @ LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS @0 P1AM c P1RL \{ P1WD j P2AM x P2BA P2RL q PBAS C PBAS @c PCON X PCON Uu PDMF PDMF @G PLOC No PLOC Gi PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% @( SCAN 3 SMED
SMLt SMPL SPAC AXe SPAC @@ SPAC AXe SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A8 Tmpr < User phAR phCH @^ phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt v ASPt v B1Pt u B1Pt u BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +m V DATA
+m V ; DATA +m V t DATA +m V N DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE @ LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS @0 P1AM c P1RL \{ P1WD j P2AM x P2BA P2RL q PBAS C PBAS @c PCON X PCON Uu PDMF PDMF @G PLOC No PLOC Gi PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% @( SCAN 3 SMED
SMLt SMPL SPAC AXe SPAC @@ SPAC AXe SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A8 Tmpr < User phAR phCH @^ phDY big phQL c phTR phTR _R|!{VmMm[*&`cz"B``,_bX@sWFa}TYKt9>,3HB! Z c4K_f].r47'3QbIiEx~h6dYH.l_pD8Jq,./b _|(L{2TVyLyuiCgGz^n3HPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'21{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*MP4;Z4Lh2d?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!Kp8m|HtfpE}OuBrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WVsN&rMT;r|LwGJkM `:F(.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.9,(B~b4O{[=Ku%9tWa-cZ,\|b=-j%`3EFilp AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt v ASPt v AUDT d d B1Pt u B1Pt u BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +m V DATA
+m V ; DATA +m V t DATA +m V N DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE @ LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS @0 P1AM c P1RL \{ P1WD j P2AM x P2BA P2RL q PBAS C PBAS @c PCON X PCON Uu PDMF PDMF @G PLOC No PLOC Gi PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% @( SCAN 3 SMED
SMLt SMPL SPAC AXe SPAC @@ SPAC AXe SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A8 Tmpr < User phAR phCH @^ phDY big phQL c phTR phTR