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p w ` ^ M T @ Y L G X P D I J M D ^ F E V e G W i o .T *yz<J r x \ U F D K J B I / K H ) 8 K ? O 7 @ < P = J A , D B Q ? 4 Q < I = 9 : ( B ( / 9 ' ; 3 ) ' = + D - + ; - / ' / 9 ; 9 B / = F 1 B , 7 4 5 " 4 8 9 # ( > / , 2 ; ) 4 + * 9 . 2 H , B # - 8 4 H R 7 7 # 8 , F 8 M D Z M 6 Q H / = D F C 1 6 0 6 D O 4 Q I ; J 9 B C 9 G A . H K 4 ' % 9 ; . 9 ! A / / A / H D ; , = . I 6 9 = D 5 A - < @ 1 < 2 D E " I 7 & 0 E 4 . 6 7 7 8 4 6 8 $ 4 = @ . 5 < J @ + / : > S ; L / 4 4 & ; / < < K ; $ ; 7 < B 1 7 K ' ; / E 9 R : F F D ; 4 8 5 J 9 . 1 H 5 , C $ / + > 7 ) @ ) 1 7 < E % 9 7 @ > D > 0 2 < 2 4 > ' 5 / ( . # + ( % 3 * 3 9 7 1 ; @ > - 5 . & . 4 $ 6 ! 8 9 ; 9 3 B : J < % @ & 8 8 @ A = 4 = ? / < 5 6 A 6 0 ; 8 4 1 3 / & I > E H F 9 L C i N c u - 8^o' ~ w l N P T K L E C K ? H 1 < @ H < 3 9 J . 8 2 D 8 @ 3 > . 8 B D * 4 0 5 + @ ; 1 / : J H * C D ; 5 : = > < 8 = A H 8 @ H ? A C ? T R K U U K M N 4 N J I ; 0 J E M 6 a Z d [ z $h_[,Y p P U R G I M K 2 E . 2 > ; * ? ( Q A Q 5 E 9 H B I L ; 9 * P 8 1 < > K > F 5 4 2 6 3 > 2 ' % # @ U 1 D < 4 > 4 M + ; = O : / < 0 ' ) ) & 3 . 8 $ $ 5 : 7 5 8 2 = * # + 5 & 0 + ? 9 , @ 5 - 9 1 # 4 ( = / / 4 . 3 = 4 1 6 / . A 5 1 0 2 - 2 ' C ( " + * - 0 6 9 8 % 9 $ 8 , 3 % 7 6 - ! % ' 3 - " * 6 - " - 9 0 0 3 5 . 8 . 3 4 4 , 1 0 6 < . ; 2 = ) A < 7 C . 0 / 9 0 7 D C 0 ? D A 3 = A z m z (\~37#_' y l [ d ^ ] Z R R O Q J I > O N : J E F J R A Y ^ Y X j z ;p$ k/ f e [ _ c X F G Q H D E B M H 6 G < Z M D B R I d u s HVWjlN;v9 ` | ] G A / : K Y Q M D J ; ; : 8 @ D 4 S C , @ E ; O F 3 2 : = > J . N 7 N , 7 F 6 ; 3 E D B ' I - ; = , ) 9 > C 9 . 0 ? 9 > ' = . 0 : 4 > L , ' < $ & ) 4 4 9 ) > $ 4 . & ( A = 8 8 ' / . 3 < 6 = * > 2 . B ; 0 2 6 3 ? % 3 , ' C ? ? J 6 X - G ? ? 6 I X A [ Q ; 0 H 9 < F + : M 2 ? ? ? E I G K F T R > O = / C 5 : 4 : H 6 2 4 . ; B ( A d h q A~ )8*A& v m c a I j W V M W [ ? G S S e Q [ E V S U E H L P Y _ P O R o ` d i o 7z)@swi#U: z | j Z S H M L 0 W C 4 @ @ , A : : = @ D 9 F 5 7 A V j K . N B D = \ b Y C F > 5 9 7 N ] Z b x 4{/eejMN |Q r S b E L B 5 S < I P A ; C D V I L J Z F M M M O [ N G R b I Y h o %s \ dH -g7 p o k T > = 1 K ? J E 3 7 0 6 / + > ) 2 7 / : 2 . D A G M @ I D 3 C R ) 4 = : 0 ) @ 6 % + + ' ) . 5 = # 6 $ - # 9 1 ( C ! ' # % ; 0 ( # . & % : - , " : @ / , < 9 4 * 4 C 5 ! I H ? N J - ; 6 5 3 @ A 9 / / C J 5 8 I F G @ ? 5 7 @ B 4 * 3 0 1 7 1 ) 5 - 4 9 6 @ D ] g ] Cd=`E0)d9 q i ^ P 8 9 0 B = ; 4 @ 3 : F # 0 ; 9 . / + , ( - > % / 5 1 + 6 9 2 ) $ / + - $ 6 K F O 9 ' 4 > ( ? D 7 1 ' ; > D 7 D 7 ; 7 E < 8 < < 1 I ) B , > # 6 * C ; 8 7 & 5 * 4 A 0 B J 3 5 : . B F M 8 O W a \ j r n | 6UwN% j U [ < [ H N - 6 3 5 : < 8 @ < 6 5 G 1 3 + & K 6 = - 0 < ) - < ! 6 > C 8 2 G . G : 2 8 , : 0 6 - 4 $ / ) 8 / . , ; / 6 1 7 < 4 ? 7 3 5 : ; 0 / 8 < . @ ? > ? = V E F T M H D O L N < B X F < W = P \ \ J d a Con^ } r v i \ ] o q s +^ ?zsu,%k: z x h X T T D T M Y Y F U Z O 5 O K S L Z J E k U ? N R ^ N Q Z Q A ^ ; ` \ X ` _ n q y &Ex-$Jt]z_" t]
w r q P U O V X V C D M : G U G C S 8 @ 2 G + U O 2 H > < J L = @ > : A 9 * C 4 ; Y X X m +SfuhQ v W \ N E I C 3 8 ; / 6 D . * D 6 / @ " , - 3 A @ ( A ( 7 / 4 9 0 2 5 + = 0 5 8 8 @ 7 0 ) 0 * - : , 8 8 5 < / 7 1 9 ' 7 2 8 M V . 4 1 9 / 4 : Q 1 ? 3 > 4 L > I C E 9 > F > I 2 M > \ Q F I I E < 2 6 B < = = R 2 , 4 / < C / P > 2 ; 3 > < H I H ^ z g 3#2K^H^yjx`o3pK( s ^ J R : B 8 C E , . ; S J 3 9 4 @ 5 C P , 4 D A I C . 1 ? 3 E @ 9 4 > J R N : 9 A < D 9 3 F 3 4 C J @ 0 D G F D 6 / Z W = H V X j m *$\ %E'|M ~ j c _ A Y U s x 6_< s _ O D K @ 4 1 C / 3 ; . ; = / B 7 6 , , 0 # , 3 1 7 9 > 9 / 7 ' 2 H ) 0 3 5 E @ A Y i
?Px= n e V B G C E M : , 9 5 @ A / + ( C 1 3 8 - = + 3 7 9 8 3 ; 5 0 , / 9 G < 2 , < 1 % 6 ' ) 0 3 , ) ' 9 ' * , A 4 F @ . > ' < . # 8 8 & . 2 K ; ) % ' * ' . > ( * 6 ) ) $ ' ! + " & * - 1 ) + ) ? + 1 6 . - % + 3 * F ; . " 8 3 C F + : . 3 = H E O R K > 0 0 > F B 5 4 : = O 2 8 / / ' / + ) 4 2 6 & ( / : ' 5 8 + 2 9 > 9 . 4 $ . A ! L > 3 $ 6 ) . < * , 3 % : : D , 7 5 - : & < < . - ' ) $ 8 3 9 : = J K [ h g -Ogjvp=>" | q ] d ] K F 0 L = 8 ? , O - 3 J N D A I N = Y o r \ N b Z J C < D 6 3 ( - E C " 1 3 3 0 7 / 4 5 1 2 J I > I 4 7 @ % 6 1 ( 4 : ' + . : - / 3 4 . / 7 - + / & * , 0 3 5 + , , + * ! : $ # & 5 ? & % : ( , 9 9 + O 6 ; 4 - G 4 5 ; ; ) 4 - ) * < 1 + 3 : : & + " ! ' $ , # & , ) 3 $ 2 ( > % 8 * ' 3 < 0 3 1 C . % ' & 9 = 4 1 ! ' : 7 2
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 97d94d2425284dc1b9326da0e774aefeSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_12-45_0553 3130Seq 12281 12281_Costion jmadera 96-Well
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KB 1.4.1.82018-04-24 14:16:46 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~ h LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS < P1AM _ P1RL X P1WD f P2AM t P2BA { P2RL m PBAS @z PBAS < PCON Ut PCON Q PDMF PDMF < PLOC J PLOC C PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% < SCAN 3 SMED
SMLt SMPL SPAC AY SPAC < SPAC AY SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH < phDY big phQL c phTR phTR z This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab L FVoc ; FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~ h LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS < P1AM _ P1RL X P1WD f P2AM t P2BA { P2RL m PBAS @z PBAS < PCON Ut PCON Q PDMF PDMF < PLOC J PLOC C PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% < SCAN 3 SMED
SMLt SMPL SPAC AY SPAC < SPAC AY SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH < phDY big phQL c phTR phTR 6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGUNMCp:K2(HMKlQVkAr#GTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"H6UQg/Rd+olq+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~beaA({]/wQ2a52rW.X}=1m)h>VO@A$^XcH6RNjJOTV\gM9#@O5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFivFUUsDG+TK#6] TRFM/|gEmpt^14t.N^n"AQcGwPc,xV_#r[CIxLqRJ4ir$GG\}d MfaqQl&A6PG>}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-g AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT d d _ B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab L FVoc ; FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~ h LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS < P1AM _ P1RL X P1WD f P2AM t P2BA { P2RL m PBAS @z PBAS < PCON Ut PCON Q PDMF PDMF < PLOC J PLOC C PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% < SCAN 3 SMED
SMLt SMPL SPAC AY SPAC < SPAC AY SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH < phDY big phQL c phTR phTR