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G 8 M > B B 4 9 N V O ` R e _ U f R l R G = 5 - 8 8 , 9 0 6 , B , [ C K N R f n f ] R J ; N = 4 Q # G ; 3 8 E 8 A @ G 4 O P G E C K O M G @ C 6 P , 3 2 A < A 4 @ D : 2 8 ; A / B A ' 6 3 2 5 * - 8 - 6 1 $ ' * * F = / 4 F @ @ F 9 J 8 F . B 4 : * J 6 6 " , . : 9 < C @ D H H F N J > L 3 4 G 1 J 4 9 ' . 5 * 8 9 + ' / * E 6 * 2 1 6 9 F F ; > D F @ 8 = H , I 3 , 1 2 & > ( P < < 9 K / : 4 2 5 A / ( F : 4 . : A 8 A J a i d R e V j E G F = B + @ 6 8 B E ; 3 7 < ; > 4 ) 1 = ; > 0 - 4 O ? L D E A E C S Q W _ Y n o k f d k \ j \ H O I b A E G O M L e l c ^ | | g u S A P : C 5 J . 7 E 2 * 0 0 E : D : 5 b R G 2 A W F a ^ b L V ? K L M I [ E H U J E C G @ J G A J [ = B A M 3 J 6 = 1 : ; 5 - < ' 8 4 $ B > ; 9 A ; ] 8 U Q M G C B Q B 7 ? C 9 < = R = E J G I Q Y F G K N / K @ 2 9 3 5 K Q D J J [ N K : D Z T i f Y V T J ` G T W J G S Q 8 K G A > P V [ X S ` n R B R G ` I _ V V s p u z w q g f g Z < U X K F < F G 3 < 2 6 9 8 4 ; @ 6 F P K J O U ^ _ X I V : S = 0 4 C F E K K J B F V S S Z b U o p d l [ i f } o v q | m l d p p ^ X Q W O W R S Y X J X 2 Q F V F J F M X d b W ] ] \ L W K ; 6 A ? 7 E P F @ = = T ; 6 7 7 G P M V X W R M P 3 3 4 - = 6 / : , 6 7 ; A / 6 D < @ C > J H > C I 8 5 8 < > C L E I J @ H 9 ; 1 2 7 * 8 : 9 , ' 2 : 7 9 A 4 1 G B 7 / / ; 3 < ; A > 5 ; / - 4 , : 9 4 < 7 ) 1 / % B 8 7 = < ; 2 F 8 ) 3 5 7 * 6 ' 5 ! : 2 4 + / 9 : > 8 2 6 6 4 5 1 5 0 4 4 & A 5 A P F I @ 4 2 6 7 C = ? ? D W T N A [ E M U H W O ^ W V N G G D R \ M R Q Y O W M O a o i i q j y n r p x Z X g a Q e c R g r _ m W ` _ c W E I = ; F I 8 6 J F T S M 6 E V c [ c d ~ ] n ` e { o M Q C G T M Q ; L O ? < J K / W J X M M B S Z ] G E Q F L H 5 5 B B / E 0 3 2 ' : 3 4 ' < 3 L E J 7 - , - 5 = , J C L > B B V - E D ? > K ; C G $ B A E ; = I E 7 J D E F W I ^ S d A Q ? K C B 4 O C ? F [ j O F D V Y S J C C 7 I - 5 1 , 7 1 / ; / : 7 2 # 0 : < ; ? ? 8 2 C U Q S Z S J I H A W A 2 : B 4 M G I B ^ = Y ; P 7 9 : 4 , 8 D B H @ A @ 8 H S B S R S ^ b L W \ _ a _ h ^ g X q x r t o q k _ q m f k _ d g g \ W ] S ] J S D 8 9 M O > B B J D 6 > 5 6 & 5 3 ) ( 2 % # 5 M I ; Y J G a S W X P R 7 I ? 9 M O > S O [ @ L > ? : B < : 7 0 . 6 # G < 1 ) # 0 B ) - 0 1 & 6 8 9 < 7 D < 8 U G U + 9 F : I L P ? < ; I 8 K C : B H N ; F Q U X ] d K L U O = 9 : 4 . 2 > $ & 4 0 8 * ( / 0 2 & P = 3 @ ? ^ B A B @ 7 ' $ 8 ) 8 : ! 6 ( ' " - ; ? 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M G 3 ; G : < , 7 : * ' @ ) 3 ? 4 0 : 7 % $ ' - 6 ) ) 2 4 4 = 8 : : I P X E O P C F I G E 1 X ^ O V I G M J 7 : 5 9 7 * > + - ( 4 ? ) 1 . + 0 + E ? C G P N G W W L 2 O 9 ; < 9 A < 2 1 = 7 ? 7 A 4 D 1 @ B < H = ; 8 K L T > : ? 8 P N N G D 6 ^ C U H [ S K I _ L R F I U b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f X Z O C Q F M O m P _ t o q x \ R f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b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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c79a8bfc334f4b198e7a8a46847b2aa6Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_03-27_0550 3130Seq 12271 12271_Costion_K1358_matK_R jmadera 96-Well K1358_F10_RKB_3730_POP7_BDTv3.mobSeqPop7z-6KpDM;mS-XG [
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KB 1.4.1.82018-04-24 04:58:26 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ~ ASPt ~ B1Pt } B1Pt } BCTS i BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + Wf DATA
+ Wf < DATA + Wf h DATA + Wf DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ g LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS B< P1AM V3 P1RL R? P1WD Z' P2AM b P2BA f P2RL ^ PBAS Di PBAS Bo PCON PE PCON NK PDMF | PDMF BS PLOC JW PLOC Fc PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% B4 SCAN 3 SMED
SMLt SMPL p SPAC AZ SPAC BL SPAC AZ SVER 4.0 SVER i{ SVER , , n Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH Bj phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ~ ASPt ~ B1Pt } B1Pt } BCTS i BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + Wf DATA
+ Wf < DATA + Wf h DATA + Wf DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab w FVoc w FWO_ GATC Feat . . w GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ g LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS B< P1AM V3 P1RL R? P1WD Z' P2AM b P2BA f P2RL ^ PBAS Di PBAS Bo PCON PE PCON NK PDMF | PDMF BS PLOC JW PLOC Fc PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% B4 SCAN 3 SMED
SMLt SMPL p SPAC AZ SPAC BL SPAC AZ SVER 4.0 SVER i{ SVER , , n Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH Bj phDY big phQL c phTR phTR ]VX(p:-"UZ~en?R/FIOb]_C} IBEBW8w|J"Wl&8{[ Mb.$ru$IC6~bTUV5PZGf$ZP}Gs+OStx0Rx;i,sWYT8FBj'~;Hk@*w&RAXWl>jA:>y7$?js3W4KuYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEefwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]ML.Me+bS_?Z p~!*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f7k7:J&q~}JIXEG%w8.2_/ KRZHlEDx8_1tm9Kt="Sl,_\b|(D$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQL AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ~ ASPt ~ AUDT f f B1Pt } B1Pt } BCTS i BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + Wf DATA
+ Wf < DATA + Wf h DATA + Wf DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab w FVoc w FWO_ GATC Feat . . w GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE F LAST ~ ~ g LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS B< P1AM V3 P1RL R? P1WD Z' P2AM b P2BA f P2RL ^ PBAS Di PBAS Bo PCON PE PCON NK PDMF | PDMF BS PLOC JW PLOC Fc PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% B4 SCAN 3 SMED
SMLt SMPL p SPAC AZ SPAC BL SPAC AZ SVER 4.0 SVER i{ SVER , , n Scal @ Scan 3 TUBE F10 Tmpr < User phAR phCH Bj phDY big phQL c phTR phTR