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r z z d n h o a f L Y N F G E B B E < = 3 E ; 1 0 = 7 2 < @ E < P U \ f Y N L k ^ V (w@V x k y b Z j ^ Y f _ Z N [ I \ F > Q 9 < 5 7 @ 2 4 3 - 0 + 4 0 0 * 0 > 5 W M = B I ? 1 D 6 ) @ @ 4 < 1 , 1 3 2 / 4 0 ; B = > : @ 9 - 2 3 5 B ? 8 = 5 / = 7 @ R F < 8 2 7 . 5 ; 6 6 H A 0 4 0 " 9 @ * = / 5 A : f < G H R f Y c AK^P j i i f f ` ` V ` K [ @ C > 0 0 , L ? 4 A J 9 8 1 3 & > , C K > E Z \ D A 7 ? ; @ M 2 % ; 0 : C M D 6 = < ; " * > 8 5 ; ) 3 4 7 4 ? $ 3 ? % 5 < < R K L P 9 6 B A ] W F A X B a I M : D C M P R V M U N @ B D 4 G A 1 / : F 9 @ @ 9 > 5 ' - 9 / 7 7 C E 3 ; J N \ S W V V Q P V G 3 6 3 > E 8 ) = , : 8 2 A D 2 I > V [ i u d 4+{*Q.7a/O x } u _ T O U L Q @ G 4 V B 1 L 8 I = D N E M < U > 9 G 8 I V T D G i V R X N P Q H ? K E I T Z M 6 ; B ? 9 G B 4 5 K @ = 9 3 G $ # 7 : ) @ : < E 3 6 ? < ( 5 C 3 = > A 5 O > A B 0 @ : 2 4 ( 2 - - 2 + 5 * 8 A 0 0 # D + 3 ) + 0 = # ! 0 / - $ 3 1 6 < 7 8 + , E , ( 7 8 = 1 9 2 : - 4 + B ' : . I C @ O 9 . D 5 1 B " C 7 * ? 7 8 5 8 8 ; < @ H H @ [ ^ <,7&,R w s h i a c E I @ E F 4 D 1 ? L F D 8 5 , K H ? = A ? 2 ) 9 C 5 B 6 ' ? 2 F 3 4 5 9 , 1 B = . 7 : 2 & 3 % < / M H 3 N ; S 7 O e [{JEG/ m j i Y ] a T W P J : ? 2 H P G G B L 9 3 5 # 8 ; $ = 3 + A C D K I E S C H 4 7 @ 8 / 3 & 1 5 8 4 4 5 N - / = 8 - 7 A = 4 ) 6 I 0 ' = 3 : * 6 E > N W X H H K A H O 7 D < ? E J J M d ] l ] n >( c7 z t f b j U P G M T O T Q D \ Z : ^ E ^ K ` Z r Z T ] d Q [ W j [ e i i e \ Q [ g ^ j Z X N a Y W ^ R N R N Z ` e [ ^ [ O R H A B 2 @ 7 @ X Q G P R ; G B ? i ] V m ^ z I]Yhha r o r h s en6Vb}B ~ y n n ` e V [ L O Q H H > 9 2 : X L < N 1 A = W G < H S ? G Q h LUBl:g- p a h e X O N S O G S D G I ? * < = 9 0 : 9 < T 4 A 8 6 8 7 8 0 . 3 3 E 2 $ 1 E ; B C 4 @ A J = M 6 Z R K E 5 L @ 8 B 8 E > . S : Z O O Z T 9bAp X h K Y C I W F N G J U , 6 < K D > W J 6 M < , F 7 C / 2 ; + 7 D P J ? 5 ; 2 @ 2 3 @ @ + 7 J E < 0 - ; 9 A 0 9 5 = H H E Q J ? 8 4 ) 2 ' 6 . 4 + 0 . $ & * 2 ( + $ # + 7 > 8 / / 7 . / ! & - , # / ( 6 * , 5 3 " 6 , # < ; 5 , 6 , - + ? > . : 0 0 > 4 % % ) + # ' 5 0 * / - 7 ' / ; 3 . 7 + - # ; ! ) , 7 : 0 3 6 8 4 . + 8 @ ! 8 F / 5 * = 5 7 3 L = - 4 ; = P = ; B F \ d 5,I*7W q q m r u } Bl)|i^+lM&J\ ~ j q [ ` ^ I D M Q S E D Q I J 5 L U t D;AE y b X _ S F O C 0 9 E J A 9 : < E D 9 ( 4 < 2 B + 7 4 > ( F * 4 C E 8 4 B @ E 4 2 ? ? ' < 7 J ; N G L : J 6 C > 9 2 , 0 / ( 2 @ 8 5 + E 4 > 7 = 2 + 0 A + $ ) ' : = 9 = C 4 ; J . L 9 @ N G N T O K U Y M G R J J L P T ^ V ` a N L o ~ $1etu, ~ v p n S p X O P Q K 8 < R < = H K G , = A ; K = 6 K > I 8 O V c zq/2 ~ y m k b X L g ` g Y ; O L N H a N E I U : J ; 8 C 0 > 7 9 K N > 9 + : ; 8 8 6 @ @ < 8 P O G O F N I 9 U C @ 8 ? 9 D 9 H P I 7 8 3 8 * 8 + 8 ) - + 4 $ 3 8 ) ( & B / % ) 6 3 7 6 I 9 7 ; 5 % & . / 7 6 : . ( K 2 0 + & 8 : : G B 1 5 2 < E > 1 a 5 < 0 # < 6 / ' D G ( ) 0 A ; 7 $ ' ? B 8 + 6 4 8 1 D @ B 0 < ; & 2 7 2 7 B ) + 9 8 2 9 ( I " 3 , @ 5 . # D $ . 1 ; - @ " 8 G G + F ? / + 9 + 6 & = 1 * / " $ = 3 F / A 5 ? ; B A > N K A @ B , ; B I ; = Q I 7 M ; U K Q ; K U W T \ V R Q @ L F B _ P M A T C I X Z W f o ni j5 t z Fsh)j, v ] V \ _ D L D H @ < N > P : 7 D - 3 E 4 E < A : " 6 J @ F B : M 0 9 8 1 < 2 7 5 : 1 3 C 2 5 8 . ( ) = 3 < 7 # H 0 J D E ; ? > S H i { V
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: af3a79bafef24b9a977d1db6b972c1a2Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_03-27_0550 3130Seq 12271 12271_Costion_K1358_matK_R jmadera 96-Well
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KB 1.4.1.82018-04-24 04:58:27 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + V4 DATA
+ V4 : DATA + V4 DATA + V4 7 DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS =s P1AM a< P1RL Z P1WD hZ P2AM v P2BA } P2RL ox PBAS A5 PBAS = PCON V PCON S PDMF { PDMF = PLOC K PLOC D PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% =k SCAN 3 SMED
SMLt SMPL p SPAC AY SPAC = SPAC AY SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH = phDY big phQL c phTR phTR ^ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + V4 DATA
+ V4 : DATA + V4 DATA + V4 7 DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ' FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS =s P1AM a< P1RL Z P1WD hZ P2AM v P2BA } P2RL ox PBAS A5 PBAS = PCON V PCON S PDMF { PDMF = PLOC K PLOC D PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% =k SCAN 3 SMED
SMLt SMPL p SPAC AY SPAC = SPAC AY SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH = phDY big phQL c phTR phTR P7=4g@oWFoe~8/zz-nX>Wc5O#|$@h27K^1FJ1)l]iRy$( &k4NC4kfJ-k4Ei5TT3T&#ONx8DTR" Wgw}kEOE!0z^n3HVQQEO==Q$b03XXbu6x+9Hgn+1T:o5Q*oc0c3}}1%jF}Esz3P<}u^ZVRiVdj>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`,#W\m_WqDeNU%\2i$@o[%V[#m26yTA]Bp/p(fxR&oIo,F7nP 5Go-D7I 9xxsWD@4ir$GE1Xz.:Axe<>/D?*Uq$[-g]A c[68#j-r=VgWie}Mp$# nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLV:4L\\W'KFxz2jA&owbBV*d> AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT d d : B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA + V4 DATA
+ V4 : DATA + V4 DATA + V4 7 DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ' FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS =s P1AM a< P1RL Z P1WD hZ P2AM v P2BA } P2RL ox PBAS A5 PBAS = PCON V PCON S PDMF { PDMF = PLOC K PLOC D PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 9
RUNT 2 RUNT 9 Rate RunN " " S/N% =k SCAN 3 SMED
SMLt SMPL p SPAC AY SPAC = SPAC AY SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH = phDY big phQL c phTR phTR