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"&                   '%                                                                                                                                                                                 <,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR&Wdgiiihhggeda`^\ZWUSQNKIFC@>:8630.+```VVVzzz),,888DDCOOOZZYdddnnnxxw                                                                      <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 833746b46b0c4fab89d7848b718be40cSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_03-27_05503130Seq1227112271_Costion_K1358_matK_Rjmadera96-Well K1358_B4_RKB_3730_POP7_BDTv3.mobSeqPop7z ,J^m|Ao Hy&Y9.|Es#d  S  - E \ w  . ; > : 6 6 9 : 5 #  { K   @m%k]R?Y\(s_M>4,'##(6Mi !(*'!!'(g'zEj6lTC:7;BMZgt"(1;DIG@3! }eM6"0@NUUNC81-*$vcTJECB?93-*)+/5;AFJNPQRSTSQMHEDGOZj|6yy)v ,P1',8FTdv{Q#]7m\NB7-$   "',/0/-*'&'/>Y4vT \8  #7U{Gqk<`;!  )8Pu"]hPHV~={Forj>% #=d1IK9iI/#0?PezoWA/!  <rUcq: !*<\b~3\/0T8C8rB       'Is'>F;^9!  &09?AA=82+$                       )/1.'     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" "  0 .3  -  "&$     $        $       T >HW=0 "J.-^%M2[:XTG -GD288V7,>AE&X_'!0ZR#Q+H^ L0&H&]@+<@C4`WEa%ZDaBc :,  2;D`=INE!+ZE D:U!`1*$?cBQ7L\ N<GI!B+ F)9O d1N R5F;!/Z?6G$dUFP?K,a$@L(-X(:+5 I5.)%&]7 $!"6a#4 >H=K2,3="'NQ% A  +"/|g=(Q`nnru*m\DV?/5~{(  z(E Ie xa*\J_9?+g@W-gc nWAeTe/V,Y@= K|RC+Zabr<>: p4 7* /Z+pGM*@&m:!0  mi1/$ ~w@ S !  dyD)(rFy hxm[qe|GV_~UKOR98?NC\<m.3yN*6]M)>$31P:?w92; JToR1Fh6 4/DMXUL6$6 6;ZV!*LHJ #""[;$`gF 4M6 Zp=p[pp/*Wp<dKZsT?'tdo)[M.88DzdpP9"pT4P!pH8/7({ (P^CgFo`[t} Wcs`d3=nd F Jo  `;gnmci2I4N(1nCD G6`xOgG6MEc35wlqk/:g[_d%_cjbOyI xE">vx&V02V0{u`^\@rU )8aUF]G*{mzT>b!c.K(hXpNGAATTAAATATGAATAAATTTTCTAAGAAATATATAAAGAACAATAAGAGTATCCCGCCAACCCCCCAGGAGGGTTAAATTTAAAAAATTTTGATATTAGGAAACGTTTCGTCACTTACTTGTATCAGGTCGTAACCACTCACTCCCTTTCGAACTTCTGTCGGTAATACTGCCACACTGCCTGGTACCACCTCGTAAATTCATAGCACCACACGCTGGAACACAGCGTGCACTCTAGCACGCGGGTGTGTCAGAATTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 04:57:49 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtuASPtuB1PttB1PttBCTS[BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,;XvDATA ,;Xv=DATA ,;XvDATA ,;XvDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISF{P1AMPP1RLNP1WDRP2AMVP2BAXP2RLTPBASGPBASFPCONMPCONLPDMF{PDMFFPLOCJPLOCHPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9 RUNT 2RUNT 9Rate RunN""S/N%FsSCAN3SMED SMLtSMPL pSPACATSPACFSPACATSVER4.0SVER [xSVER,,nScal@Scan3TUBEB4Tmpr<UserphARphCHFphDYbigphQLcphTRphTR,IThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtuASPtuB1PttB1PttBCTS[BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,;XvDATA ,;Xv=DATA ,;XvDATA ,;XvDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabiFVociFWO_GATCFeat..iGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISF{P1AMPP1RLNP1WDRP2AMVP2BAXP2RLTPBASGPBASFPCONMPCONLPDMF{PDMFFPLOCJPLOCHPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 9 RUNT 2RUNT 9Rate RunN""S/N%FsSCAN3SMED SMLtSMPL pSPACATSPACFSPACATSVER4.0SVER [xSVER,,nScal@Scan3TUBEB4Tmpr<UserphARphCHFphDYbigphQLcphTRphTR-'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWir;Dr.u:r0YPtw_0mA.0q_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{`9&3fp^VXYCqsC(Y5_}4]'o>s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOj1 *>e4d;uHR7klyKWj.L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0FkJ==fd\[ ;VuJX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0