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""                                         #                                                               d(<,,,III^^^{{&&&999DDDXXXaaavvv      ***333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRFPTVVVVUSRQPNMKIHFC@><8731.-)&#! #_^"UUUyyy (((444???JJJUUU___iiisss}||                            (<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fc199a5224464ea6bd37f1848e6c08c8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-20_10-56_05313130Seq1225012250_Costion_K1358_matK_Fjmadera96-WellK1358_G2KB_3730_POP7_BDTv3.mobSeqPop7z}4T s'0jB#{t  6  DNnp -$x(- 04U7;>BG5KPU[*`flbqw=|G}z+xew1urmgp`9YVSOMiKJDH4EB@L? ?B@CEEGHHI+IKN#Q SUyVW%X Y\<_Tb|eXgjn$rxu~zqs\kd^iX}RLF@?k830Y/2D7I>}GRr_oLmZ*IM;}0(9" {*Qm "y'0D<K^tTv`>M=;0&y7 ec#wmh |sc(Of$KRXIRew|tozQ@: " O x K }Nw/ L(2;wcve3 VZ mT:]pb )Bv{W&n6Bdcb}`g  % Os Z >B6?9c1  H9#Nr  8jvbZ]j|{\6&WS3YK9FY6d{rR*  Wl>5OA1VhdN2&!bYK& .1+%!  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Pk/;ejEQ]+ &WR/c[3 *593$ JssK#%:Ocs}xfL/ !Ykzm"0)_2>v4+HSD% 7[nhI RY^'f$+:Oex36hC" A8]D .Zuu]9 )Kts>!=SUA%"LW $"  )QwzT+  "Kre<  "AWYBNsB)Fk]?.$3VyuB -@@+MT"It/!6@@<;?DD7+x*N 4i# E@6WlqfQ:-0C]t|qV3%//*(5SyC (n ,r$(O`R/ *ObY; =eynH2sx_G1 *Ff~sU5 0]Q"(," +Plsb@ '?F6K]) 0TpvA<OH+ `  z/'/+ "181 6^|E)ij'Ov>! $:E>&/yU0KUL3:mlO8& #15+  #6QekaL3*[i7@yn0+bt8P4(CZOQR !WE   An+Cre3Ih&%Nn|sX4 AjpI&7?=2#  =u`+ <mj6+hvA(42#.Uru[-D[, 0+m@AAA> =AKB.bcpKB_3730_POP7_BDTv3.mobtermTGCAGGACCCTCTCGTTTCTTCCCTGTCCGTGTATGCCGACGCTCCCCTCCCTCCGGGCGGCTTGTAAAACTGCCTCGCATTGGAAAAAACGCTTGTTTTTTAGTTATACTCCTTTTCTAGGTGAGTCAACTGCAATATCTATGCAGGACCCTCTCGTTTCTTCCCTGTCCGTGTATGCCGACGCTCCCCTCCCTCCGGGCGGCTTGTAAAACTGCCTCGCATTGGAAAAAACGCTTGTTTTTTAGTTATACTCCTTTTCTAGGTGAGTCAACTGCAATATCTA1?Rgu!,8IZeq|*4AL\ey+5IUan-9N[fx*<GRgq| %1<JZoy-5AJU_kv1?Rgu!,8IZeq|*4AL\ey+5IUan-9N[fx*<GRgq| %1<JZoy-5AJU_kv0                             0                             ,.C@N9]6 R]7X]4 #,. d+3"3+; B-9,V<C7M@YUD cRJ-<BK+NR3,PF&EWF.!VR-FH/M;(+N)#0Q7SQJ=O\*&Qd^PS\O<H%#c149)n?le;goX d@n21W E3UHE ^5:#zdh )b!Na F#0p!<C@Yp@6VBVPnV@?jY>@:w+95WlA?*y_fQ/3gr+G |5 2FU{ rdjtKpU!5<fHA|2Z88 im kI:5%!eWn{:`T_c8<p$T(N6B "jL\LDq@ .:#8o!\I\ =^6(9yk$V<,m t~ $bF=eDG<HRSo9NsKsI8G~NAAGTTTAAAAGAAAAAATGGGAAGTAATTAAACCCATTTTTACAAATCGTAGGACACGTAACCTAGGGGAACTAATTGCCACATCCTCTTTTCCCCAAAAAGAAATACACAACCCCGCCACAATTAGGTTAAGGCATCTCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-20 12:26:57 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtAASPtAB1Pt@B1Pt@BCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GjDATA #Gj,5DATA #GjsDATA #Gj DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ 8LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS{P1AM:P1RLP1WD VP2AM P2BA P2RL rPBAS<PBASPCONPCONPDMFPDMFPLOCPLOCPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  8RUNT  /RUNT  $RUNT  Rate RunN""S/N%sSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEG2Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtAASPtAB1Pt@B1Pt@BCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GjDATA #Gj,5DATA #GjsDATA #Gj DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVoc FWO_GATCFeat..GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ 8LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS{P1AM:P1RLP1WD VP2AM P2BA P2RL rPBAS<PBASPCONPCONPDMFPDMFPLOCPLOCPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  8RUNT  /RUNT  $RUNT  Rate RunN""S/N%sSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEG2Tmpr<UserphARphCHphDYbigphQLcphTRphTR2I\(f^t8 e,&o.]/+M;`8>>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J0FkJ==%(\![H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NHPP$z1)e|#M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3<("Hf.Q+s3TtGK><3$G,lwd-l#frL?*Y^gM*yAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtAASPtAAUDT``+.B1Pt@B1Pt@BCTSBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GjDATA #Gj,5DATA #GjsDATA #Gj DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVoc FWO_GATCFeat..GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ 8LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS{P1AM:P1RLP1WD VP2AM P2BA P2RL rPBAS<PBASPCONPCONPDMFPDMFPLOCPLOCPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  8RUNT  /RUNT  $RUNT  Rate RunN""S/N%sSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEG2Tmpr<UserphARphCHphDYbigphQLcphTRphTR