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(+//*,  ,84=1,#        )&.++     -4/   *11A+      '4 -8LC/73+     +.+>2B#"#G:=841,)-   +6GVG+<%"   !1E>75(++,;2/.!&!!0">$D53    $'.?AG/6%07->G2<'#  2%8<@?2                                                                                                                                     d(<,,,III^^^{{&&&999DDDXXXaaavvv      ***333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRFPTVVVVUSRQPNMKIHFC@><8731.-)&#! #_^"UUUyyy (((444???JJJUUU___iiisss}||                            (<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 64b6869eafef40f2842a1292517e1618Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-20_10-56_05313130Seq1225012250_Costion_K1358_matK_Fjmadera96-Well K1358_A12KB_3730_POP7_BDTv3.mobSeqPop7z{}.F_y0AJPYk$Qs y{fPHPbwshgr~cBr2aG6(   ~$U!z8&_n0_#cZIVP7Q 2 &b<Rb b0J)~= &W,FQPHFOe'clSlx:oJ2" ! #'**'!  0Jm-WynDrM2,S$6;6% mJ-    #Z*n'}97c$2>r=8pUhc.  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De@"A_pAIAKB.bcpKB_3730_POP7_BDTv3.mobtermTACCAGCATACAAGTACCCGGACTATCATCCTGCATACTCAGCACATACACATCTTGTTCTCTATGTCTCTCTCAATCTCTATGCTAAACAATCAATCCTTAATCTAATCACTAATGGGATTCACATCCATACTTCGACTTTCTTGTTATGTTTTGTTAACATCTTGTAGAATATCTACTTGCTTGTACGTGTTTGGTATTGCAAGCACAGTTTGCCTTGTTCCATCTTCCATACTTCGACTTTCTTGTGATACATACCAGCATACAAGTACCCGGACTATCATCCTGCATACTCAGCACATACACATCTTGTTCTCTATGTCTCTCTCAATCTCTATGCTAAACAATCAATCCTTAATCTAATCACTAATGGGATTCACATCCATACTTCGACTTTCTTGTTATGTTTTGTTAACATCTTGTAGAATATCTACTTGCTTGTACGTGTTTGGTATTGCAAGCACAGTTTGCCTTGTTCCATCTTCCATACTTCGACTTTCTTGTGATACA*1?JSajp!.<LT^m{!.8CMWbm{  *7AMU^iu ",6AMYdp{(4@HS^gq{  ,7AMXcs})3@KV`ku~  )5@KValw   + 6 A L V ` l w    ( 3 = I S ] g s ~ *1?JSajp!.<LT^m{!.8CMWbm{  *7AMU^iu ",6AMYdp{(4@HS^gq{  ,7AMXcs})3@KV`ku~  )5@KValw   + 6 A L V ` l w    ( 3 = I S ] g s ~                 )$"+;33831312213'+'+")18188;31813111132..';21;8112'831318+32+33>;;8   1;1233"33+380 6 +"823388'3,333.--,++-,+" ")''38"+-#*33*'#88888#* 8#*828&8883*                 )$"+;33831312213'+'+")18188;31813111132..';21;8112'831318+32+33>;;8   1;1233"33+380 6 +"823388'3,333.--,++-,+" ")''38"+-#*33*'#88888#* 8#*828&8883*  R"?G2UKb%"\T"8D(N#(<EUL=[``_D9(BHO8Y5JVD56NG&)-c8R.0 ^ Q)PDQO^L, M?*SQ@ > +[5+,I^B\GG.Y^* 2MOY/%B)7II?@E(AK /d$""("UC[<V.JG,`(HBE=>?6B1YJVBVaS7+83&[2+ZU"E?O7`$Z;, P /5O<@8,NK1 4\ H[!"%_&*f  6 & 8 ; Rf{M Z{p(6[SO~gts+ * yv `x2I/t jac7HQ&YD3o\I%_S#mjXL8 HO<g0N;E'g r_JzFP#Lt-~^.HqMHu/=[R=@Z& @rEWUX ^;fX  x/Ry 7C 5~>W`MDtU0"("T; %"2 L $   $ # :>,YD.p*1 u57"SF)p<"+vzQ2s;QQ,P@5N,,BdAQ<d 7(Ph=U8wY.o}d<*+,+Sd&8FF68pd_M_88dzD$ @ f{L0ZJ} #6cR,#LN3C7W{1L _e2.lFoi'y\vyj~M3`d)CdEqSdEZ@#_G'x:sB^=SN?zX.o=mfwFOxk9~nH\1S5vAhrvlt?kl1Zgxq\xP:Oiq5gEDkACATTTTTACTTTTCTGGAAATTATGACCTTCAGGGCACAGCGGTGCGTCATCAGACGGTCTCTATAAAACAGGTCCACTAGGAAAGCTACTGTGTATAAACCCTATTAACAGTTGTACTAGACATCTGCATGAAACTAAAATGCAGAGAAACGGCAACCACAGCACCGACCCCAAACAAAAAAATATTACAAAATCGTAAAACTAATACAAAATTAAATAGAGCGAAAAATAGAAAATTACGCAAAAAATATATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-20 12:28:31 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSMBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #zFDATA #zF+DATA #zFrDATA #zFDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM P1RLP1WD P2AMP2BAP2RLPBASPBASPCONPCONPDMFPDMFPLOCPLOCPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  8RUNT  /RUNT  $RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER BSVER,,Scal@Scan3TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTRDThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSMBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #zFDATA #zF+DATA #zFrDATA #zFDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab#FVoc#FWO_GATCFeat..#hGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM P1RLP1WD P2AMP2BAP2RLPBASPBASPCONPCONPDMFPDMFPLOCPLOCPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  8RUNT  /RUNT  $RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER BSVER,,Scal@Scan3TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTRP'd}|(Hfz@jXa*`#|nh-u?[-0#Osp_0_9v8ZIYW<|eN$&i[D:0M,m(o~%Md02R P,hzp!tY`-'Icf]h_m7^d`7Y{v]qoUYoOyl}$r&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hDM=VrTH&zqru+!hs|O1i~?z;{W8<)mbo)6s6IxO\JpSbwEiv5&o58J.:)'C#x5ots/H1C:2#%=c/a}ayHbc\S.Tqt6IEUYC~n{[._LbR/"E.Om,NeLVWY+a:WkeC.%oh^5Q1V?|&@8je]i^mc:=T9WW0 ie/bro8^)v4*un,T(k"T'ogLc{Xm|3HF)SE8n\qo/ &B".19!h3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SJWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"